Job ID = 2010912 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,460,817 reads read : 17,460,817 reads written : 17,460,817 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1103929.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 17460817 reads; of these: 17460817 (100.00%) were unpaired; of these: 442481 (2.53%) aligned 0 times 14444575 (82.73%) aligned exactly 1 time 2573761 (14.74%) aligned >1 times 97.47% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6817348 / 17018336 = 0.4006 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:21:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:21:32: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:21:32: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:21:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:21:33: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:21:33: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:21:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:21:34: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:21:34: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:21:40: 1000000 INFO @ Sat, 06 Jul 2019 01:21:42: 1000000 INFO @ Sat, 06 Jul 2019 01:21:45: 1000000 INFO @ Sat, 06 Jul 2019 01:21:47: 2000000 INFO @ Sat, 06 Jul 2019 01:21:53: 2000000 INFO @ Sat, 06 Jul 2019 01:21:54: 3000000 INFO @ Sat, 06 Jul 2019 01:21:56: 2000000 INFO @ Sat, 06 Jul 2019 01:22:01: 4000000 INFO @ Sat, 06 Jul 2019 01:22:04: 3000000 INFO @ Sat, 06 Jul 2019 01:22:06: 3000000 INFO @ Sat, 06 Jul 2019 01:22:08: 5000000 INFO @ Sat, 06 Jul 2019 01:22:14: 4000000 INFO @ Sat, 06 Jul 2019 01:22:16: 6000000 INFO @ Sat, 06 Jul 2019 01:22:16: 4000000 INFO @ Sat, 06 Jul 2019 01:22:23: 7000000 INFO @ Sat, 06 Jul 2019 01:22:24: 5000000 INFO @ Sat, 06 Jul 2019 01:22:27: 5000000 INFO @ Sat, 06 Jul 2019 01:22:30: 8000000 INFO @ Sat, 06 Jul 2019 01:22:35: 6000000 INFO @ Sat, 06 Jul 2019 01:22:37: 9000000 INFO @ Sat, 06 Jul 2019 01:22:38: 6000000 INFO @ Sat, 06 Jul 2019 01:22:44: 10000000 INFO @ Sat, 06 Jul 2019 01:22:46: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:22:46: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:22:46: #1 total tags in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:22:46: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:22:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:22:46: #1 tags after filtering in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:22:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:22:46: #1 finished! INFO @ Sat, 06 Jul 2019 01:22:46: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:22:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:22:46: 7000000 INFO @ Sat, 06 Jul 2019 01:22:47: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:22:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:22:47: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:22:49: 7000000 INFO @ Sat, 06 Jul 2019 01:22:58: 8000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:23:00: 8000000 INFO @ Sat, 06 Jul 2019 01:23:09: 9000000 INFO @ Sat, 06 Jul 2019 01:23:10: 9000000 INFO @ Sat, 06 Jul 2019 01:23:19: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:23:21: 10000000 INFO @ Sat, 06 Jul 2019 01:23:21: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:23:21: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:23:21: #1 total tags in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:23:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:23:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:23:22: #1 tags after filtering in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:23:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:23:22: #1 finished! INFO @ Sat, 06 Jul 2019 01:23:22: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:23:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:23:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:23:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:23:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:23:23: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:23:23: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:23:23: #1 total tags in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:23:23: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:23:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:23:23: #1 tags after filtering in treatment: 10200988 INFO @ Sat, 06 Jul 2019 01:23:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:23:23: #1 finished! INFO @ Sat, 06 Jul 2019 01:23:23: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:23:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:23:24: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:23:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:23:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423154/SRX423154.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。