Job ID = 2010903 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,552,050 reads read : 37,104,100 reads written : 18,552,050 reads 0-length : 18,552,050 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:28 18552050 reads; of these: 18552050 (100.00%) were unpaired; of these: 544781 (2.94%) aligned 0 times 17183295 (92.62%) aligned exactly 1 time 823974 (4.44%) aligned >1 times 97.06% overall alignment rate Time searching: 00:03:28 Overall time: 00:03:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9124789 / 18007269 = 0.5067 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:27:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:27:26: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:27:26: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:27:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:27:26: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:27:26: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:27:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:27:28: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:27:28: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:27:34: 1000000 INFO @ Sat, 06 Jul 2019 01:27:35: 1000000 INFO @ Sat, 06 Jul 2019 01:27:36: 1000000 INFO @ Sat, 06 Jul 2019 01:27:43: 2000000 INFO @ Sat, 06 Jul 2019 01:27:43: 2000000 INFO @ Sat, 06 Jul 2019 01:27:44: 2000000 INFO @ Sat, 06 Jul 2019 01:27:51: 3000000 INFO @ Sat, 06 Jul 2019 01:27:52: 3000000 INFO @ Sat, 06 Jul 2019 01:27:52: 3000000 INFO @ Sat, 06 Jul 2019 01:27:59: 4000000 INFO @ Sat, 06 Jul 2019 01:28:02: 4000000 INFO @ Sat, 06 Jul 2019 01:28:02: 4000000 INFO @ Sat, 06 Jul 2019 01:28:07: 5000000 INFO @ Sat, 06 Jul 2019 01:28:09: 5000000 INFO @ Sat, 06 Jul 2019 01:28:10: 5000000 INFO @ Sat, 06 Jul 2019 01:28:14: 6000000 INFO @ Sat, 06 Jul 2019 01:28:17: 6000000 INFO @ Sat, 06 Jul 2019 01:28:19: 6000000 INFO @ Sat, 06 Jul 2019 01:28:22: 7000000 INFO @ Sat, 06 Jul 2019 01:28:24: 7000000 INFO @ Sat, 06 Jul 2019 01:28:27: 7000000 INFO @ Sat, 06 Jul 2019 01:28:29: 8000000 INFO @ Sat, 06 Jul 2019 01:28:31: 8000000 INFO @ Sat, 06 Jul 2019 01:28:36: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:28:36: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:28:36: #1 total tags in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:36: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:28:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:28:36: #1 tags after filtering in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:28:36: #1 finished! INFO @ Sat, 06 Jul 2019 01:28:36: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:28:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:28:36: 8000000 INFO @ Sat, 06 Jul 2019 01:28:37: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:28:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:28:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10_peaks.narrowPeak: No such file or directory BedGraph に変換しました。 pass1 - making usageList (0 chroms): 2 millis BigWig に変換中... needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:28:38: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:28:38: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:28:38: #1 total tags in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:38: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:28:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:28:38: #1 tags after filtering in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:28:38: #1 finished! INFO @ Sat, 06 Jul 2019 01:28:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:28:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:28:39: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:28:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:28:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:28:43: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:28:43: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:28:43: #1 total tags in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:28:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:28:43: #1 tags after filtering in treatment: 8882480 INFO @ Sat, 06 Jul 2019 01:28:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:28:43: #1 finished! INFO @ Sat, 06 Jul 2019 01:28:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:28:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:28:44: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:28:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:28:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225342/SRX4225342.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。