Job ID = 2010895 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,049,825 reads read : 38,099,650 reads written : 19,049,825 reads 0-length : 19,049,825 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:43 19049825 reads; of these: 19049825 (100.00%) were unpaired; of these: 439288 (2.31%) aligned 0 times 14792300 (77.65%) aligned exactly 1 time 3818237 (20.04%) aligned >1 times 97.69% overall alignment rate Time searching: 00:03:43 Overall time: 00:03:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8859382 / 18610537 = 0.4760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:24:15: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:24:15: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:24:15: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:24:15: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:24:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:24:16: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:24:16: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:24:22: 1000000 INFO @ Sat, 06 Jul 2019 01:24:27: 1000000 INFO @ Sat, 06 Jul 2019 01:24:28: 1000000 INFO @ Sat, 06 Jul 2019 01:24:29: 2000000 INFO @ Sat, 06 Jul 2019 01:24:36: 3000000 INFO @ Sat, 06 Jul 2019 01:24:37: 2000000 INFO @ Sat, 06 Jul 2019 01:24:38: 2000000 INFO @ Sat, 06 Jul 2019 01:24:43: 4000000 INFO @ Sat, 06 Jul 2019 01:24:48: 3000000 INFO @ Sat, 06 Jul 2019 01:24:49: 3000000 INFO @ Sat, 06 Jul 2019 01:24:49: 5000000 INFO @ Sat, 06 Jul 2019 01:24:56: 6000000 INFO @ Sat, 06 Jul 2019 01:24:58: 4000000 INFO @ Sat, 06 Jul 2019 01:24:59: 4000000 INFO @ Sat, 06 Jul 2019 01:25:03: 7000000 INFO @ Sat, 06 Jul 2019 01:25:08: 5000000 INFO @ Sat, 06 Jul 2019 01:25:09: 5000000 INFO @ Sat, 06 Jul 2019 01:25:09: 8000000 INFO @ Sat, 06 Jul 2019 01:25:16: 9000000 INFO @ Sat, 06 Jul 2019 01:25:18: 6000000 INFO @ Sat, 06 Jul 2019 01:25:19: 6000000 INFO @ Sat, 06 Jul 2019 01:25:21: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:25:21: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:25:21: #1 total tags in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:25:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:25:21: #1 tags after filtering in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:25:21: #1 finished! INFO @ Sat, 06 Jul 2019 01:25:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:25:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:25:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:25:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:25:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:25:29: 7000000 INFO @ Sat, 06 Jul 2019 01:25:30: 7000000 INFO @ Sat, 06 Jul 2019 01:25:39: 8000000 INFO @ Sat, 06 Jul 2019 01:25:40: 8000000 INFO @ Sat, 06 Jul 2019 01:25:49: 9000000 INFO @ Sat, 06 Jul 2019 01:25:50: 9000000 INFO @ Sat, 06 Jul 2019 01:25:56: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:25:56: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:25:56: #1 total tags in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:56: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:25:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:25:57: #1 tags after filtering in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:25:57: #1 finished! INFO @ Sat, 06 Jul 2019 01:25:57: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:25:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:25:57: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:25:57: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:25:57: #1 total tags in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:57: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:25:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:25:57: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:25:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:25:57: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:25:57: #1 tags after filtering in treatment: 9751155 INFO @ Sat, 06 Jul 2019 01:25:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:25:57: #1 finished! INFO @ Sat, 06 Jul 2019 01:25:57: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:25:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:25:58: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:25:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:25:58: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05_model.r’pass1 - making usageList (0 chroms): No such file or directory: 4 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05_*.xls’: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225336/SRX4225336.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。