Job ID = 2010892 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,328,516 reads read : 62,657,032 reads written : 31,328,516 reads 0-length : 31,328,516 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:28 31328516 reads; of these: 31328516 (100.00%) were unpaired; of these: 1034032 (3.30%) aligned 0 times 26386883 (84.23%) aligned exactly 1 time 3907601 (12.47%) aligned >1 times 96.70% overall alignment rate Time searching: 00:05:28 Overall time: 00:05:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14101985 / 30294484 = 0.4655 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:30:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:30:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:30:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:30:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:30:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:30:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:30:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:30:33: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:30:33: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:30:37: 1000000 INFO @ Sat, 06 Jul 2019 01:30:38: 1000000 INFO @ Sat, 06 Jul 2019 01:30:41: 1000000 INFO @ Sat, 06 Jul 2019 01:30:43: 2000000 INFO @ Sat, 06 Jul 2019 01:30:44: 2000000 INFO @ Sat, 06 Jul 2019 01:30:49: 3000000 INFO @ Sat, 06 Jul 2019 01:30:50: 2000000 INFO @ Sat, 06 Jul 2019 01:30:50: 3000000 INFO @ Sat, 06 Jul 2019 01:30:55: 4000000 INFO @ Sat, 06 Jul 2019 01:30:56: 4000000 INFO @ Sat, 06 Jul 2019 01:30:58: 3000000 INFO @ Sat, 06 Jul 2019 01:31:01: 5000000 INFO @ Sat, 06 Jul 2019 01:31:02: 5000000 INFO @ Sat, 06 Jul 2019 01:31:05: 4000000 INFO @ Sat, 06 Jul 2019 01:31:07: 6000000 INFO @ Sat, 06 Jul 2019 01:31:08: 6000000 INFO @ Sat, 06 Jul 2019 01:31:13: 5000000 INFO @ Sat, 06 Jul 2019 01:31:14: 7000000 INFO @ Sat, 06 Jul 2019 01:31:14: 7000000 INFO @ Sat, 06 Jul 2019 01:31:20: 8000000 INFO @ Sat, 06 Jul 2019 01:31:21: 8000000 INFO @ Sat, 06 Jul 2019 01:31:21: 6000000 INFO @ Sat, 06 Jul 2019 01:31:26: 9000000 INFO @ Sat, 06 Jul 2019 01:31:27: 9000000 INFO @ Sat, 06 Jul 2019 01:31:29: 7000000 INFO @ Sat, 06 Jul 2019 01:31:32: 10000000 INFO @ Sat, 06 Jul 2019 01:31:33: 10000000 INFO @ Sat, 06 Jul 2019 01:31:37: 8000000 INFO @ Sat, 06 Jul 2019 01:31:38: 11000000 INFO @ Sat, 06 Jul 2019 01:31:39: 11000000 INFO @ Sat, 06 Jul 2019 01:31:44: 12000000 INFO @ Sat, 06 Jul 2019 01:31:45: 9000000 INFO @ Sat, 06 Jul 2019 01:31:45: 12000000 INFO @ Sat, 06 Jul 2019 01:31:50: 13000000 INFO @ Sat, 06 Jul 2019 01:31:51: 13000000 INFO @ Sat, 06 Jul 2019 01:31:53: 10000000 INFO @ Sat, 06 Jul 2019 01:31:56: 14000000 INFO @ Sat, 06 Jul 2019 01:31:57: 14000000 INFO @ Sat, 06 Jul 2019 01:32:01: 11000000 INFO @ Sat, 06 Jul 2019 01:32:02: 15000000 INFO @ Sat, 06 Jul 2019 01:32:03: 15000000 INFO @ Sat, 06 Jul 2019 01:32:08: 16000000 INFO @ Sat, 06 Jul 2019 01:32:08: 12000000 INFO @ Sat, 06 Jul 2019 01:32:09: 16000000 INFO @ Sat, 06 Jul 2019 01:32:09: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:32:09: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:32:09: #1 total tags in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:09: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:10: #1 tags after filtering in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:10: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:10: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:10: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:32:10: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:32:10: #1 total tags in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:10: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:11: #1 tags after filtering in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:11: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:11: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:11: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:32:12: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:32:16: 13000000 INFO @ Sat, 06 Jul 2019 01:32:24: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:32:31: 15000000 INFO @ Sat, 06 Jul 2019 01:32:39: 16000000 INFO @ Sat, 06 Jul 2019 01:32:40: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:32:40: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:32:40: #1 total tags in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:40: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:41: #1 tags after filtering in treatment: 16192499 INFO @ Sat, 06 Jul 2019 01:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:41: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:41: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:42: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4225333/SRX4225333.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。