Job ID = 11296968 sra ファイルのダウンロード中... Completed: 191117K bytes transferred in 5 seconds (300677K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 6484875 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4212610/SRR7310242.sra Written 6484875 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4212610/SRR7310242.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:24 6484875 reads; of these: 6484875 (100.00%) were unpaired; of these: 3704908 (57.13%) aligned 0 times 2639426 (40.70%) aligned exactly 1 time 140541 (2.17%) aligned >1 times 42.87% overall alignment rate Time searching: 00:01:24 Overall time: 00:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 658327 / 2779967 = 0.2368 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 18:04:54: # Command line: callpeak -t SRX4212610.bam -f BAM -g 12100000 -n SRX4212610.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4212610.05 # format = BAM # ChIP-seq file = ['SRX4212610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:04:54: # Command line: callpeak -t SRX4212610.bam -f BAM -g 12100000 -n SRX4212610.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4212610.20 # format = BAM # ChIP-seq file = ['SRX4212610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:04:54: # Command line: callpeak -t SRX4212610.bam -f BAM -g 12100000 -n SRX4212610.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4212610.10 # format = BAM # ChIP-seq file = ['SRX4212610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:04:54: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:04:54: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:04:54: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:04:54: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:04:54: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:04:54: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:05:02: 1000000 INFO @ Mon, 05 Nov 2018 18:05:03: 1000000 INFO @ Mon, 05 Nov 2018 18:05:03: 1000000 INFO @ Mon, 05 Nov 2018 18:05:10: 2000000 INFO @ Mon, 05 Nov 2018 18:05:11: #1 tag size is determined as 60 bps INFO @ Mon, 05 Nov 2018 18:05:11: #1 tag size = 60 INFO @ Mon, 05 Nov 2018 18:05:11: #1 total tags in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:11: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:05:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:05:11: #1 tags after filtering in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:05:11: #1 finished! INFO @ Mon, 05 Nov 2018 18:05:11: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:05:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:05:11: #2 number of paired peaks: 20 WARNING @ Mon, 05 Nov 2018 18:05:11: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:05:11: Process for pairing-model is terminated! cat: SRX4212610.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4212610.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 18:05:13: 2000000 INFO @ Mon, 05 Nov 2018 18:05:13: 2000000 INFO @ Mon, 05 Nov 2018 18:05:14: #1 tag size is determined as 60 bps INFO @ Mon, 05 Nov 2018 18:05:14: #1 tag size = 60 INFO @ Mon, 05 Nov 2018 18:05:14: #1 total tags in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:14: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:05:14: #1 tag size is determined as 60 bps INFO @ Mon, 05 Nov 2018 18:05:14: #1 tag size = 60 INFO @ Mon, 05 Nov 2018 18:05:14: #1 total tags in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:14: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:05:14: #1 tags after filtering in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:05:14: #1 finished! INFO @ Mon, 05 Nov 2018 18:05:14: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:05:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:05:14: #1 tags after filtering in treatment: 2121640 INFO @ Mon, 05 Nov 2018 18:05:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:05:14: #1 finished! INFO @ Mon, 05 Nov 2018 18:05:14: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:05:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:05:14: #2 number of paired peaks: 20 WARNING @ Mon, 05 Nov 2018 18:05:14: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:05:14: Process for pairing-model is terminated! cat: SRX4212610.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4212610.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 18:05:14: #2 number of paired peaks: 20 WARNING @ Mon, 05 Nov 2018 18:05:14: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:05:14: Process for pairing-model is terminated! cat: SRX4212610.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4212610.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4212610.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。