Job ID = 2010874 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,218,365 reads read : 10,436,730 reads written : 5,218,365 reads 0-length : 5,218,365 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 5218365 reads; of these: 5218365 (100.00%) were unpaired; of these: 201080 (3.85%) aligned 0 times 4154645 (79.62%) aligned exactly 1 time 862640 (16.53%) aligned >1 times 96.15% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1703458 / 5017285 = 0.3395 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:14:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:14:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:14:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:14:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:14:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:14:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:15:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:15:00: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:15:00: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:15:09: 1000000 INFO @ Sat, 06 Jul 2019 01:15:11: 1000000 INFO @ Sat, 06 Jul 2019 01:15:11: 1000000 INFO @ Sat, 06 Jul 2019 01:15:18: 2000000 INFO @ Sat, 06 Jul 2019 01:15:24: 2000000 INFO @ Sat, 06 Jul 2019 01:15:24: 2000000 INFO @ Sat, 06 Jul 2019 01:15:27: 3000000 INFO @ Sat, 06 Jul 2019 01:15:30: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:15:30: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:15:30: #1 total tags in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:30: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:15:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:15:30: #1 tags after filtering in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:15:30: #1 finished! INFO @ Sat, 06 Jul 2019 01:15:30: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:15:30: #2 number of paired peaks: 45 WARNING @ Sat, 06 Jul 2019 01:15:30: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:15:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:15:36: 3000000 INFO @ Sat, 06 Jul 2019 01:15:37: 3000000 INFO @ Sat, 06 Jul 2019 01:15:40: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:15:40: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:15:40: #1 total tags in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:40: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:15:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:15:40: #1 tags after filtering in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:15:40: #1 finished! INFO @ Sat, 06 Jul 2019 01:15:40: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:15:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:15:41: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:15:41: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:15:41: #1 total tags in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:41: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:15:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:15:41: #1 tags after filtering in treatment: 3313827 INFO @ Sat, 06 Jul 2019 01:15:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:15:41: #1 finished! INFO @ Sat, 06 Jul 2019 01:15:41: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:15:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:15:41: #2 number of paired peaks: 45 WARNING @ Sat, 06 Jul 2019 01:15:41: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:15:41: #2 number of paired peaks: 45 WARNING @ Sat, 06 Jul 2019 01:15:41: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:15:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140973/SRX4140973.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。