Job ID = 2010860 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T16:00:16 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 5,553,681 reads read : 11,107,362 reads written : 5,553,681 reads 0-length : 5,553,681 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:07 5553681 reads; of these: 5553681 (100.00%) were unpaired; of these: 546765 (9.85%) aligned 0 times 4446138 (80.06%) aligned exactly 1 time 560778 (10.10%) aligned >1 times 90.15% overall alignment rate Time searching: 00:02:07 Overall time: 00:02:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1534309 / 5006916 = 0.3064 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:07:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:07:56: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:07:56: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:07:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:07:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:07:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:07:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:07:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:07:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:08:08: 1000000 INFO @ Sat, 06 Jul 2019 01:08:08: 1000000 INFO @ Sat, 06 Jul 2019 01:08:09: 1000000 INFO @ Sat, 06 Jul 2019 01:08:19: 2000000 INFO @ Sat, 06 Jul 2019 01:08:20: 2000000 INFO @ Sat, 06 Jul 2019 01:08:21: 2000000 INFO @ Sat, 06 Jul 2019 01:08:29: 3000000 INFO @ Sat, 06 Jul 2019 01:08:32: 3000000 INFO @ Sat, 06 Jul 2019 01:08:34: 3000000 INFO @ Sat, 06 Jul 2019 01:08:34: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:08:34: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:08:34: #1 total tags in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:08:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:08:34: #1 tags after filtering in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:08:34: #1 finished! INFO @ Sat, 06 Jul 2019 01:08:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:08:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:08:35: #2 number of paired peaks: 93 WARNING @ Sat, 06 Jul 2019 01:08:35: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:08:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:08:37: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:08:37: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:08:37: #1 total tags in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:37: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:08:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:08:37: #1 tags after filtering in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:08:37: #1 finished! INFO @ Sat, 06 Jul 2019 01:08:37: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:08:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:08:38: #2 number of paired peaks: 93 WARNING @ Sat, 06 Jul 2019 01:08:38: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:08:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:08:39: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:08:39: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:08:39: #1 total tags in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:39: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:08:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:08:39: #1 tags after filtering in treatment: 3472607 INFO @ Sat, 06 Jul 2019 01:08:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:08:39: #1 finished! INFO @ Sat, 06 Jul 2019 01:08:39: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:08:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:08:40: #2 number of paired peaks: 93 WARNING @ Sat, 06 Jul 2019 01:08:40: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:08:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140962/SRX4140962.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。