Job ID = 2010842 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,734,694 reads read : 11,469,388 reads written : 5,734,694 reads 0-length : 5,734,694 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:55 5734694 reads; of these: 5734694 (100.00%) were unpaired; of these: 449597 (7.84%) aligned 0 times 4626955 (80.68%) aligned exactly 1 time 658142 (11.48%) aligned >1 times 92.16% overall alignment rate Time searching: 00:01:55 Overall time: 00:01:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1071954 / 5285097 = 0.2028 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:05:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:05:07: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:05:07: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:05:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:05:08: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:05:08: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:05:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:05:09: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:05:09: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:05:18: 1000000 INFO @ Sat, 06 Jul 2019 01:05:20: 1000000 INFO @ Sat, 06 Jul 2019 01:05:22: 1000000 INFO @ Sat, 06 Jul 2019 01:05:29: 2000000 INFO @ Sat, 06 Jul 2019 01:05:31: 2000000 INFO @ Sat, 06 Jul 2019 01:05:35: 2000000 INFO @ Sat, 06 Jul 2019 01:05:40: 3000000 INFO @ Sat, 06 Jul 2019 01:05:42: 3000000 INFO @ Sat, 06 Jul 2019 01:05:47: 3000000 INFO @ Sat, 06 Jul 2019 01:05:50: 4000000 INFO @ Sat, 06 Jul 2019 01:05:53: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:05:53: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:05:53: #1 total tags in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:05:53: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:53: #1 tags after filtering in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:05:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:53: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:53: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:53: 4000000 INFO @ Sat, 06 Jul 2019 01:05:53: #2 number of paired peaks: 41 WARNING @ Sat, 06 Jul 2019 01:05:53: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:53: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:05:55: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:05:55: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:05:55: #1 total tags in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:05:55: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:55: #1 tags after filtering in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:05:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:55: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:55: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:56: #2 number of paired peaks: 41 WARNING @ Sat, 06 Jul 2019 01:05:56: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:56: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:06:00: 4000000 INFO @ Sat, 06 Jul 2019 01:06:02: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:06:02: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:06:02: #1 total tags in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:06:02: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:06:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:06:02: #1 tags after filtering in treatment: 4213143 INFO @ Sat, 06 Jul 2019 01:06:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:06:02: #1 finished! INFO @ Sat, 06 Jul 2019 01:06:02: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:06:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:06:03: #2 number of paired peaks: 41 WARNING @ Sat, 06 Jul 2019 01:06:03: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:06:03: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20_peaks.narrowPeak : No such file or directoryBigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140948/SRX4140948.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。