Job ID = 2010814 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 39,722,447 reads read : 79,444,894 reads written : 39,722,447 reads 0-length : 39,722,447 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:53 39722447 reads; of these: 39722447 (100.00%) were unpaired; of these: 2706753 (6.81%) aligned 0 times 32536741 (81.91%) aligned exactly 1 time 4478953 (11.28%) aligned >1 times 93.19% overall alignment rate Time searching: 00:16:53 Overall time: 00:16:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 23019449 / 37015694 = 0.6219 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:45:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:06: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:06: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:07: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:07: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:08: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:08: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:16: 1000000 INFO @ Sat, 06 Jul 2019 01:45:18: 1000000 INFO @ Sat, 06 Jul 2019 01:45:19: 1000000 INFO @ Sat, 06 Jul 2019 01:45:25: 2000000 INFO @ Sat, 06 Jul 2019 01:45:30: 2000000 INFO @ Sat, 06 Jul 2019 01:45:31: 2000000 INFO @ Sat, 06 Jul 2019 01:45:34: 3000000 INFO @ Sat, 06 Jul 2019 01:45:40: 3000000 INFO @ Sat, 06 Jul 2019 01:45:41: 3000000 INFO @ Sat, 06 Jul 2019 01:45:44: 4000000 INFO @ Sat, 06 Jul 2019 01:45:50: 4000000 INFO @ Sat, 06 Jul 2019 01:45:52: 4000000 INFO @ Sat, 06 Jul 2019 01:45:53: 5000000 INFO @ Sat, 06 Jul 2019 01:46:01: 5000000 INFO @ Sat, 06 Jul 2019 01:46:02: 6000000 INFO @ Sat, 06 Jul 2019 01:46:02: 5000000 INFO @ Sat, 06 Jul 2019 01:46:11: 6000000 INFO @ Sat, 06 Jul 2019 01:46:11: 7000000 INFO @ Sat, 06 Jul 2019 01:46:13: 6000000 INFO @ Sat, 06 Jul 2019 01:46:20: 8000000 INFO @ Sat, 06 Jul 2019 01:46:22: 7000000 INFO @ Sat, 06 Jul 2019 01:46:24: 7000000 INFO @ Sat, 06 Jul 2019 01:46:30: 9000000 INFO @ Sat, 06 Jul 2019 01:46:32: 8000000 INFO @ Sat, 06 Jul 2019 01:46:35: 8000000 INFO @ Sat, 06 Jul 2019 01:46:39: 10000000 INFO @ Sat, 06 Jul 2019 01:46:43: 9000000 INFO @ Sat, 06 Jul 2019 01:46:45: 9000000 INFO @ Sat, 06 Jul 2019 01:46:48: 11000000 INFO @ Sat, 06 Jul 2019 01:46:53: 10000000 INFO @ Sat, 06 Jul 2019 01:46:56: 10000000 INFO @ Sat, 06 Jul 2019 01:46:57: 12000000 INFO @ Sat, 06 Jul 2019 01:47:03: 11000000 INFO @ Sat, 06 Jul 2019 01:47:06: 11000000 INFO @ Sat, 06 Jul 2019 01:47:07: 13000000 INFO @ Sat, 06 Jul 2019 01:47:14: 12000000 INFO @ Sat, 06 Jul 2019 01:47:16: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:47:16: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:47:16: #1 total tags in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:16: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:47:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:47:16: #1 tags after filtering in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:47:16: #1 finished! INFO @ Sat, 06 Jul 2019 01:47:16: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:47:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:47:17: 12000000 INFO @ Sat, 06 Jul 2019 01:47:17: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:47:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:47:17: Process for pairing-model is terminated! BedGraph に変換しました。 INFO @ Sat, 06 Jul 2019 01:47:24: 13000000 INFO @ Sat, 06 Jul 2019 01:47:27: 13000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:47:34: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:47:34: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:47:34: #1 total tags in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:47:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:47:35: #1 tags after filtering in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:47:35: #1 finished! INFO @ Sat, 06 Jul 2019 01:47:35: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:47:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:47:36: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:47:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:47:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:47:37: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:47:37: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:47:37: #1 total tags in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:37: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:47:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:47:38: #1 tags after filtering in treatment: 13996245 INFO @ Sat, 06 Jul 2019 01:47:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:47:38: #1 finished! INFO @ Sat, 06 Jul 2019 01:47:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:47:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:47:39: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:47:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:47:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140923/SRX4140923.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。