Job ID = 2010811 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.52' 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.52' spots read : 5,545,155 reads read : 11,090,310 reads written : 5,545,155 reads 0-length : 5,545,155 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:56 5545155 reads; of these: 5545155 (100.00%) were unpaired; of these: 576098 (10.39%) aligned 0 times 4380444 (79.00%) aligned exactly 1 time 588613 (10.61%) aligned >1 times 89.61% overall alignment rate Time searching: 00:01:56 Overall time: 00:01:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1039252 / 4969057 = 0.2091 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:53:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:53:54: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:53:54: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:53:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:53:55: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:53:55: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:53:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:53:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:53:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:54:04: 1000000 INFO @ Sat, 06 Jul 2019 00:54:05: 1000000 INFO @ Sat, 06 Jul 2019 00:54:07: 1000000 INFO @ Sat, 06 Jul 2019 00:54:13: 2000000 INFO @ Sat, 06 Jul 2019 00:54:14: 2000000 INFO @ Sat, 06 Jul 2019 00:54:16: 2000000 INFO @ Sat, 06 Jul 2019 00:54:23: 3000000 INFO @ Sat, 06 Jul 2019 00:54:23: 3000000 INFO @ Sat, 06 Jul 2019 00:54:25: 3000000 INFO @ Sat, 06 Jul 2019 00:54:32: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:54:32: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:54:32: #1 total tags in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:54:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:54:32: #1 tags after filtering in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:54:32: #1 finished! INFO @ Sat, 06 Jul 2019 00:54:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:54:32: #2 number of paired peaks: 50 WARNING @ Sat, 06 Jul 2019 00:54:32: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:54:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 06 Jul 2019 00:54:32: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:54:32: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:54:32: #1 total tags in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:54:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:54:32: #1 tags after filtering in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:54:32: #1 finished! INFO @ Sat, 06 Jul 2019 00:54:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:54:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:54:32: #2 number of paired peaks: 50 WARNING @ Sat, 06 Jul 2019 00:54:32: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:54:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:54:34: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:54:34: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:54:34: #1 total tags in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:54:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:54:34: #1 tags after filtering in treatment: 3929805 INFO @ Sat, 06 Jul 2019 00:54:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:54:34: #1 finished! INFO @ Sat, 06 Jul 2019 00:54:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:54:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:54:34: #2 number of paired peaks: 50 WARNING @ Sat, 06 Jul 2019 00:54:34: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:54:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140920/SRX4140920.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。