Job ID = 2010809 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.91' 2019-07-05T15:43:54 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.91' spots read : 5,651,566 reads read : 11,303,132 reads written : 5,651,566 reads 0-length : 5,651,566 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:37 5651566 reads; of these: 5651566 (100.00%) were unpaired; of these: 896606 (15.86%) aligned 0 times 3371991 (59.66%) aligned exactly 1 time 1382969 (24.47%) aligned >1 times 84.14% overall alignment rate Time searching: 00:01:37 Overall time: 00:01:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1700886 / 4754960 = 0.3577 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:51:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:28: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:28: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:38: 1000000 INFO @ Sat, 06 Jul 2019 00:51:39: 1000000 INFO @ Sat, 06 Jul 2019 00:51:42: 1000000 INFO @ Sat, 06 Jul 2019 00:51:46: 2000000 INFO @ Sat, 06 Jul 2019 00:51:48: 2000000 INFO @ Sat, 06 Jul 2019 00:51:52: 2000000 INFO @ Sat, 06 Jul 2019 00:51:53: 3000000 INFO @ Sat, 06 Jul 2019 00:51:54: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:51:54: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:51:54: #1 total tags in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:51:54: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:51:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:51:54: #1 tags after filtering in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:51:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:51:54: #1 finished! INFO @ Sat, 06 Jul 2019 00:51:54: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:51:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:51:54: #2 number of paired peaks: 31 WARNING @ Sat, 06 Jul 2019 00:51:54: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:51:54: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:51:56: 3000000 INFO @ Sat, 06 Jul 2019 00:51:57: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:51:57: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:51:57: #1 total tags in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:51:57: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:51:57: #1 tags after filtering in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:51:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:51:57: #1 finished! INFO @ Sat, 06 Jul 2019 00:51:57: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:51:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:51:57: #2 number of paired peaks: 31 WARNING @ Sat, 06 Jul 2019 00:51:57: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:51:57: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:52:02: 3000000 INFO @ Sat, 06 Jul 2019 00:52:02: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:52:02: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:52:02: #1 total tags in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:52:02: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:52:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:52:02: #1 tags after filtering in treatment: 3054074 INFO @ Sat, 06 Jul 2019 00:52:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:52:02: #1 finished! INFO @ Sat, 06 Jul 2019 00:52:02: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:52:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:52:03: #2 number of paired peaks: 31 WARNING @ Sat, 06 Jul 2019 00:52:03: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:52:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10_peaks.narrowPeak: No such file or directorycut: /home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05_*.xls’: No such file or directory pass1 - making usageList (0 chroms): 1 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.05_peaks.narrowPeak’: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140919/SRX4140919.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。