Job ID = 2010800 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,192,509 reads read : 26,385,018 reads written : 13,192,509 reads 0-length : 13,192,509 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:26 13192509 reads; of these: 13192509 (100.00%) were unpaired; of these: 1179092 (8.94%) aligned 0 times 10241711 (77.63%) aligned exactly 1 time 1771706 (13.43%) aligned >1 times 91.06% overall alignment rate Time searching: 00:04:26 Overall time: 00:04:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4666142 / 12013417 = 0.3884 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:59:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:59:14: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:59:14: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:59:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:59:15: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:59:15: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:59:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:59:16: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:59:16: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:59:24: 1000000 INFO @ Sat, 06 Jul 2019 00:59:24: 1000000 INFO @ Sat, 06 Jul 2019 00:59:29: 1000000 INFO @ Sat, 06 Jul 2019 00:59:33: 2000000 INFO @ Sat, 06 Jul 2019 00:59:33: 2000000 INFO @ Sat, 06 Jul 2019 00:59:41: 3000000 INFO @ Sat, 06 Jul 2019 00:59:42: 2000000 INFO @ Sat, 06 Jul 2019 00:59:43: 3000000 INFO @ Sat, 06 Jul 2019 00:59:49: 4000000 INFO @ Sat, 06 Jul 2019 00:59:51: 4000000 INFO @ Sat, 06 Jul 2019 00:59:54: 3000000 INFO @ Sat, 06 Jul 2019 00:59:57: 5000000 INFO @ Sat, 06 Jul 2019 01:00:00: 5000000 INFO @ Sat, 06 Jul 2019 01:00:05: 6000000 INFO @ Sat, 06 Jul 2019 01:00:05: 4000000 INFO @ Sat, 06 Jul 2019 01:00:09: 6000000 INFO @ Sat, 06 Jul 2019 01:00:13: 7000000 INFO @ Sat, 06 Jul 2019 01:00:16: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:00:16: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:00:16: #1 total tags in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:16: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:00:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:00:16: #1 tags after filtering in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:00:16: #1 finished! INFO @ Sat, 06 Jul 2019 01:00:16: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:00:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:00:17: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:00:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:00:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:00:17: 5000000 INFO @ Sat, 06 Jul 2019 01:00:18: 7000000 INFO @ Sat, 06 Jul 2019 01:00:21: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:00:21: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:00:21: #1 total tags in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:00:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:00:21: #1 tags after filtering in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:00:21: #1 finished! INFO @ Sat, 06 Jul 2019 01:00:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:00:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:00:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:00:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:00:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:00:28: 6000000 INFO @ Sat, 06 Jul 2019 01:00:39: 7000000 INFO @ Sat, 06 Jul 2019 01:00:43: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:00:43: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:00:43: #1 total tags in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:00:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:00:43: #1 tags after filtering in treatment: 7347275 INFO @ Sat, 06 Jul 2019 01:00:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:00:43: #1 finished! INFO @ Sat, 06 Jul 2019 01:00:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:00:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:00:44: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:00:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:00:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140911/SRX4140911.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。