Job ID = 2010799 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T15:40:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T15:41:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T15:41:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 14,844,980 reads read : 29,689,960 reads written : 14,844,980 reads 0-length : 14,844,980 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:31 14844980 reads; of these: 14844980 (100.00%) were unpaired; of these: 1596121 (10.75%) aligned 0 times 11593224 (78.10%) aligned exactly 1 time 1655635 (11.15%) aligned >1 times 89.25% overall alignment rate Time searching: 00:05:31 Overall time: 00:05:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4290195 / 13248859 = 0.3238 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:03:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:45: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:45: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:47: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:47: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:59: 1000000 INFO @ Sat, 06 Jul 2019 01:04:00: 1000000 INFO @ Sat, 06 Jul 2019 01:04:02: 1000000 INFO @ Sat, 06 Jul 2019 01:04:12: 2000000 INFO @ Sat, 06 Jul 2019 01:04:13: 2000000 INFO @ Sat, 06 Jul 2019 01:04:17: 2000000 INFO @ Sat, 06 Jul 2019 01:04:24: 3000000 INFO @ Sat, 06 Jul 2019 01:04:25: 3000000 INFO @ Sat, 06 Jul 2019 01:04:29: 3000000 INFO @ Sat, 06 Jul 2019 01:04:35: 4000000 INFO @ Sat, 06 Jul 2019 01:04:36: 4000000 INFO @ Sat, 06 Jul 2019 01:04:41: 4000000 INFO @ Sat, 06 Jul 2019 01:04:47: 5000000 INFO @ Sat, 06 Jul 2019 01:04:48: 5000000 INFO @ Sat, 06 Jul 2019 01:04:52: 5000000 INFO @ Sat, 06 Jul 2019 01:04:58: 6000000 INFO @ Sat, 06 Jul 2019 01:04:59: 6000000 INFO @ Sat, 06 Jul 2019 01:05:04: 6000000 INFO @ Sat, 06 Jul 2019 01:05:10: 7000000 INFO @ Sat, 06 Jul 2019 01:05:10: 7000000 INFO @ Sat, 06 Jul 2019 01:05:15: 7000000 INFO @ Sat, 06 Jul 2019 01:05:21: 8000000 INFO @ Sat, 06 Jul 2019 01:05:22: 8000000 INFO @ Sat, 06 Jul 2019 01:05:26: 8000000 INFO @ Sat, 06 Jul 2019 01:05:32: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:05:32: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:05:32: #1 total tags in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:33: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:05:33: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:05:33: #1 total tags in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:33: #1 tags after filtering in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:33: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:33: #1 tags after filtering in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:33: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:33: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:33: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:05:33: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:05:37: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 01:05:37: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 01:05:37: #1 total tags in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:37: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:37: #1 tags after filtering in treatment: 8958664 INFO @ Sat, 06 Jul 2019 01:05:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:37: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:37: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:38: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140910/SRX4140910.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。