Job ID = 2010798 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T15:45:55 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T15:45:55 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 5,567,788 reads read : 11,135,576 reads written : 5,567,788 reads 0-length : 5,567,788 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:02 5567788 reads; of these: 5567788 (100.00%) were unpaired; of these: 368296 (6.61%) aligned 0 times 4533582 (81.43%) aligned exactly 1 time 665910 (11.96%) aligned >1 times 93.39% overall alignment rate Time searching: 00:02:02 Overall time: 00:02:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1225186 / 5199492 = 0.2356 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:51:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:27: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:27: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:28: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:28: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:51:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:51:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:51:42: 1000000 INFO @ Sat, 06 Jul 2019 00:51:43: 1000000 INFO @ Sat, 06 Jul 2019 00:51:45: 1000000 INFO @ Sat, 06 Jul 2019 00:51:56: 2000000 INFO @ Sat, 06 Jul 2019 00:52:00: 2000000 INFO @ Sat, 06 Jul 2019 00:52:01: 2000000 INFO @ Sat, 06 Jul 2019 00:52:09: 3000000 INFO @ Sat, 06 Jul 2019 00:52:15: 3000000 INFO @ Sat, 06 Jul 2019 00:52:17: 3000000 INFO @ Sat, 06 Jul 2019 00:52:23: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:52:23: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:52:23: #1 total tags in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:23: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:52:23: #1 tags after filtering in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:52:23: #1 finished! INFO @ Sat, 06 Jul 2019 00:52:23: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:52:23: #2 number of paired peaks: 29 WARNING @ Sat, 06 Jul 2019 00:52:23: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:52:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:52:30: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:52:30: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:52:30: #1 total tags in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:30: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:52:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:52:30: #1 tags after filtering in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:52:30: #1 finished! INFO @ Sat, 06 Jul 2019 00:52:30: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:52:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:52:31: #2 number of paired peaks: 29 WARNING @ Sat, 06 Jul 2019 00:52:31: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:52:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:52:32: #1 tag size is determined as 100 bps INFO @ Sat, 06 Jul 2019 00:52:32: #1 tag size = 100 INFO @ Sat, 06 Jul 2019 00:52:32: #1 total tags in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:52:32: #1 tags after filtering in treatment: 3974306 INFO @ Sat, 06 Jul 2019 00:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:52:32: #1 finished! INFO @ Sat, 06 Jul 2019 00:52:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:52:32: #2 number of paired peaks: 29 WARNING @ Sat, 06 Jul 2019 00:52:32: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:52:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140909/SRX4140909.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。