Job ID = 2010795 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,803,167 reads read : 23,606,334 reads written : 11,803,167 reads 0-length : 11,803,167 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:36 11803167 reads; of these: 11803167 (100.00%) were unpaired; of these: 1522725 (12.90%) aligned 0 times 9087005 (76.99%) aligned exactly 1 time 1193437 (10.11%) aligned >1 times 87.10% overall alignment rate Time searching: 00:04:36 Overall time: 00:04:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2838114 / 10280442 = 0.2761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:58:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:58:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:58:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:58:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:58:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:58:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:58:38: 1000000 INFO @ Sat, 06 Jul 2019 00:58:39: 1000000 INFO @ Sat, 06 Jul 2019 00:58:47: 2000000 INFO @ Sat, 06 Jul 2019 00:58:48: 2000000 INFO @ Sat, 06 Jul 2019 00:58:55: 3000000 INFO @ Sat, 06 Jul 2019 00:58:56: 3000000 INFO @ Sat, 06 Jul 2019 00:59:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:59:02: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:59:02: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:59:04: 4000000 INFO @ Sat, 06 Jul 2019 00:59:05: 4000000 INFO @ Sat, 06 Jul 2019 00:59:11: 1000000 INFO @ Sat, 06 Jul 2019 00:59:12: 5000000 INFO @ Sat, 06 Jul 2019 00:59:13: 5000000 INFO @ Sat, 06 Jul 2019 00:59:18: 2000000 INFO @ Sat, 06 Jul 2019 00:59:20: 6000000 INFO @ Sat, 06 Jul 2019 00:59:22: 6000000 INFO @ Sat, 06 Jul 2019 00:59:26: 3000000 INFO @ Sat, 06 Jul 2019 00:59:29: 7000000 INFO @ Sat, 06 Jul 2019 00:59:30: 7000000 INFO @ Sat, 06 Jul 2019 00:59:32: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 00:59:32: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 00:59:32: #1 total tags in treatment: 7442328 INFO @ Sat, 06 Jul 2019 00:59:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:59:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:59:33: #1 tags after filtering in treatment: 7442328 INFO @ Sat, 06 Jul 2019 00:59:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:59:33: #1 finished! INFO @ Sat, 06 Jul 2019 00:59:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:59:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:59:33: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:59:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:59:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:59:34: 4000000 INFO @ Sat, 06 Jul 2019 00:59:34: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 00:59:34: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 00:59:34: #1 total tags in treatment: 7442328 INFO @ Sat, 06 Jul 2019 00:59:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:59:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:59:34: #1 tags after filtering in treatment: 7442328 INFO @ Sat, 06 Jul 2019 00:59:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:59:34: #1 finished! INFO @ Sat, 06 Jul 2019 00:59:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:59:35: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:59:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:59:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:59:42: 5000000 INFO @ Sat, 06 Jul 2019 00:59:50: 6000000 INFO @ Sat, 06 Jul 2019 00:59:58: 7000000 INFO @ Sat, 06 Jul 2019 01:00:01: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:00:01: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:00:01: #1 total tags in treatment: 7442328 INFO @ Sat, 06 Jul 2019 01:00:01: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:00:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:00:01: #1 tags after filtering in treatment: 7442328 INFO @ Sat, 06 Jul 2019 01:00:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:00:01: #1 finished! INFO @ Sat, 06 Jul 2019 01:00:01: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:00:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:00:02: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:00:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:00:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4140906/SRX4140906.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。