Job ID = 14521277 SRX = SRX4108766 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26184744 spots for SRR7192195/SRR7192195.sra Written 26184744 spots for SRR7192195/SRR7192195.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:18 26184744 reads; of these: 26184744 (100.00%) were unpaired; of these: 3747647 (14.31%) aligned 0 times 20418834 (77.98%) aligned exactly 1 time 2018263 (7.71%) aligned >1 times 85.69% overall alignment rate Time searching: 00:10:18 Overall time: 00:10:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 10840301 / 22437097 = 0.4831 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:05:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:05:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:05:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:05:57: 1000000 INFO @ Sat, 15 Jan 2022 21:06:03: 2000000 INFO @ Sat, 15 Jan 2022 21:06:10: 3000000 INFO @ Sat, 15 Jan 2022 21:06:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:22: 5000000 INFO @ Sat, 15 Jan 2022 21:06:27: 1000000 INFO @ Sat, 15 Jan 2022 21:06:29: 6000000 INFO @ Sat, 15 Jan 2022 21:06:33: 2000000 INFO @ Sat, 15 Jan 2022 21:06:35: 7000000 INFO @ Sat, 15 Jan 2022 21:06:40: 3000000 INFO @ Sat, 15 Jan 2022 21:06:41: 8000000 INFO @ Sat, 15 Jan 2022 21:06:46: 4000000 INFO @ Sat, 15 Jan 2022 21:06:47: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:52: 5000000 INFO @ Sat, 15 Jan 2022 21:06:54: 10000000 INFO @ Sat, 15 Jan 2022 21:06:59: 6000000 INFO @ Sat, 15 Jan 2022 21:06:59: 1000000 INFO @ Sat, 15 Jan 2022 21:07:00: 11000000 INFO @ Sat, 15 Jan 2022 21:07:04: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:07:04: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:07:04: #1 total tags in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:07:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:04: #1 tags after filtering in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:07:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:07:04: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:05: 7000000 INFO @ Sat, 15 Jan 2022 21:07:07: 2000000 INFO @ Sat, 15 Jan 2022 21:07:11: 8000000 INFO @ Sat, 15 Jan 2022 21:07:15: 3000000 INFO @ Sat, 15 Jan 2022 21:07:18: 9000000 INFO @ Sat, 15 Jan 2022 21:07:23: 4000000 INFO @ Sat, 15 Jan 2022 21:07:24: 10000000 INFO @ Sat, 15 Jan 2022 21:07:31: 11000000 INFO @ Sat, 15 Jan 2022 21:07:31: 5000000 INFO @ Sat, 15 Jan 2022 21:07:35: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:07:35: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:07:35: #1 total tags in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:07:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:07:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:07:35: #1 tags after filtering in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:07:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:07:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:07:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:07:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:07:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:07:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:07:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:07:39: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:07:47: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:07:55: 8000000 INFO @ Sat, 15 Jan 2022 21:08:03: 9000000 INFO @ Sat, 15 Jan 2022 21:08:11: 10000000 INFO @ Sat, 15 Jan 2022 21:08:18: 11000000 INFO @ Sat, 15 Jan 2022 21:08:23: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:08:23: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:08:23: #1 total tags in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:08:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:23: #1 tags after filtering in treatment: 11596796 INFO @ Sat, 15 Jan 2022 21:08:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:08:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108766/SRX4108766.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling