Job ID = 14521276 SRX = SRX4108765 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27348769 spots for SRR7192194/SRR7192194.sra Written 27348769 spots for SRR7192194/SRR7192194.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:13 27348769 reads; of these: 27348769 (100.00%) were unpaired; of these: 5984664 (21.88%) aligned 0 times 19042641 (69.63%) aligned exactly 1 time 2321464 (8.49%) aligned >1 times 78.12% overall alignment rate Time searching: 00:15:13 Overall time: 00:15:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8814692 / 21364105 = 0.4126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:54: 1000000 INFO @ Sat, 15 Jan 2022 21:14:03: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:13: 3000000 INFO @ Sat, 15 Jan 2022 21:14:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:22: 4000000 INFO @ Sat, 15 Jan 2022 21:14:25: 1000000 INFO @ Sat, 15 Jan 2022 21:14:31: 5000000 INFO @ Sat, 15 Jan 2022 21:14:35: 2000000 INFO @ Sat, 15 Jan 2022 21:14:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:45: 3000000 INFO @ Sat, 15 Jan 2022 21:14:49: 7000000 INFO @ Sat, 15 Jan 2022 21:14:55: 1000000 INFO @ Sat, 15 Jan 2022 21:14:56: 4000000 INFO @ Sat, 15 Jan 2022 21:14:58: 8000000 INFO @ Sat, 15 Jan 2022 21:15:05: 2000000 INFO @ Sat, 15 Jan 2022 21:15:08: 5000000 INFO @ Sat, 15 Jan 2022 21:15:08: 9000000 INFO @ Sat, 15 Jan 2022 21:15:15: 3000000 INFO @ Sat, 15 Jan 2022 21:15:18: 6000000 INFO @ Sat, 15 Jan 2022 21:15:18: 10000000 INFO @ Sat, 15 Jan 2022 21:15:25: 4000000 INFO @ Sat, 15 Jan 2022 21:15:28: 11000000 INFO @ Sat, 15 Jan 2022 21:15:28: 7000000 INFO @ Sat, 15 Jan 2022 21:15:34: 5000000 INFO @ Sat, 15 Jan 2022 21:15:38: 12000000 INFO @ Sat, 15 Jan 2022 21:15:39: 8000000 INFO @ Sat, 15 Jan 2022 21:15:43: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:15:43: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:15:43: #1 total tags in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:15:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:43: #1 tags after filtering in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:15:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:44: 6000000 INFO @ Sat, 15 Jan 2022 21:15:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:49: 9000000 INFO @ Sat, 15 Jan 2022 21:15:53: 7000000 INFO @ Sat, 15 Jan 2022 21:15:59: 10000000 INFO @ Sat, 15 Jan 2022 21:16:02: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:16:08: 11000000 INFO @ Sat, 15 Jan 2022 21:16:12: 9000000 INFO @ Sat, 15 Jan 2022 21:16:18: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:16:22: 10000000 INFO @ Sat, 15 Jan 2022 21:16:24: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:16:24: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:16:24: #1 total tags in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:16:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:25: #1 tags after filtering in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:16:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:32: 11000000 INFO @ Sat, 15 Jan 2022 21:16:40: 12000000 INFO @ Sat, 15 Jan 2022 21:16:45: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:16:45: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:16:45: #1 total tags in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:16:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:45: #1 tags after filtering in treatment: 12549413 INFO @ Sat, 15 Jan 2022 21:16:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108765/SRX4108765.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling