Job ID = 14521274 SRX = SRX4108763 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27179774 spots for SRR7192192/SRR7192192.sra Written 27179774 spots for SRR7192192/SRR7192192.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:08 27179774 reads; of these: 27179774 (100.00%) were unpaired; of these: 6471565 (23.81%) aligned 0 times 18123970 (66.68%) aligned exactly 1 time 2584239 (9.51%) aligned >1 times 76.19% overall alignment rate Time searching: 00:11:08 Overall time: 00:11:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8653848 / 20708209 = 0.4179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:41: 1000000 INFO @ Sat, 15 Jan 2022 21:11:49: 2000000 INFO @ Sat, 15 Jan 2022 21:11:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:05: 4000000 INFO @ Sat, 15 Jan 2022 21:12:14: 1000000 INFO @ Sat, 15 Jan 2022 21:12:15: 5000000 INFO @ Sat, 15 Jan 2022 21:12:25: 6000000 INFO @ Sat, 15 Jan 2022 21:12:25: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:35: 7000000 INFO @ Sat, 15 Jan 2022 21:12:37: 3000000 INFO @ Sat, 15 Jan 2022 21:12:44: 1000000 INFO @ Sat, 15 Jan 2022 21:12:45: 8000000 INFO @ Sat, 15 Jan 2022 21:12:48: 4000000 INFO @ Sat, 15 Jan 2022 21:12:55: 9000000 INFO @ Sat, 15 Jan 2022 21:12:56: 2000000 INFO @ Sat, 15 Jan 2022 21:12:59: 5000000 INFO @ Sat, 15 Jan 2022 21:13:05: 10000000 INFO @ Sat, 15 Jan 2022 21:13:08: 3000000 INFO @ Sat, 15 Jan 2022 21:13:11: 6000000 INFO @ Sat, 15 Jan 2022 21:13:16: 11000000 INFO @ Sat, 15 Jan 2022 21:13:19: 4000000 INFO @ Sat, 15 Jan 2022 21:13:22: 7000000 INFO @ Sat, 15 Jan 2022 21:13:26: 12000000 INFO @ Sat, 15 Jan 2022 21:13:26: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:13:26: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:13:26: #1 total tags in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:13:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:27: #1 tags after filtering in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:13:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:13:27: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:13:30: 5000000 INFO @ Sat, 15 Jan 2022 21:13:33: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:13:41: 6000000 INFO @ Sat, 15 Jan 2022 21:13:44: 9000000 INFO @ Sat, 15 Jan 2022 21:13:52: 7000000 INFO @ Sat, 15 Jan 2022 21:13:55: 10000000 INFO @ Sat, 15 Jan 2022 21:14:03: 8000000 INFO @ Sat, 15 Jan 2022 21:14:06: 11000000 INFO @ Sat, 15 Jan 2022 21:14:14: 9000000 INFO @ Sat, 15 Jan 2022 21:14:17: 12000000 INFO @ Sat, 15 Jan 2022 21:14:17: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:14:17: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:14:17: #1 total tags in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:14:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:17: #1 tags after filtering in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:14:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:14:24: 10000000 INFO @ Sat, 15 Jan 2022 21:14:34: 11000000 INFO @ Sat, 15 Jan 2022 21:14:45: 12000000 INFO @ Sat, 15 Jan 2022 21:14:45: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:14:45: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:14:45: #1 total tags in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:14:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:45: #1 tags after filtering in treatment: 12054361 INFO @ Sat, 15 Jan 2022 21:14:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:45: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108763/SRX4108763.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling