Job ID = 14521272 SRX = SRX4108761 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 38883207 spots for SRR7192190/SRR7192190.sra Written 38883207 spots for SRR7192190/SRR7192190.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:44 38883207 reads; of these: 38883207 (100.00%) were unpaired; of these: 17191656 (44.21%) aligned 0 times 18969383 (48.79%) aligned exactly 1 time 2722168 (7.00%) aligned >1 times 55.79% overall alignment rate Time searching: 00:15:44 Overall time: 00:15:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9988983 / 21691551 = 0.4605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:42: 1000000 INFO @ Sat, 15 Jan 2022 21:12:51: 2000000 INFO @ Sat, 15 Jan 2022 21:13:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:10: 4000000 INFO @ Sat, 15 Jan 2022 21:13:14: 1000000 INFO @ Sat, 15 Jan 2022 21:13:21: 5000000 INFO @ Sat, 15 Jan 2022 21:13:25: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:33: 6000000 INFO @ Sat, 15 Jan 2022 21:13:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:37: 3000000 INFO @ Sat, 15 Jan 2022 21:13:45: 7000000 INFO @ Sat, 15 Jan 2022 21:13:46: 1000000 INFO @ Sat, 15 Jan 2022 21:13:49: 4000000 INFO @ Sat, 15 Jan 2022 21:13:57: 8000000 INFO @ Sat, 15 Jan 2022 21:13:58: 2000000 INFO @ Sat, 15 Jan 2022 21:14:01: 5000000 INFO @ Sat, 15 Jan 2022 21:14:09: 9000000 INFO @ Sat, 15 Jan 2022 21:14:11: 3000000 INFO @ Sat, 15 Jan 2022 21:14:13: 6000000 INFO @ Sat, 15 Jan 2022 21:14:22: 10000000 INFO @ Sat, 15 Jan 2022 21:14:24: 4000000 INFO @ Sat, 15 Jan 2022 21:14:25: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:14:34: 11000000 INFO @ Sat, 15 Jan 2022 21:14:37: 5000000 INFO @ Sat, 15 Jan 2022 21:14:37: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:14:43: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:14:43: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:14:43: #1 total tags in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:14:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:43: #1 tags after filtering in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:14:49: 9000000 INFO @ Sat, 15 Jan 2022 21:14:50: 6000000 INFO @ Sat, 15 Jan 2022 21:15:01: 10000000 INFO @ Sat, 15 Jan 2022 21:15:04: 7000000 INFO @ Sat, 15 Jan 2022 21:15:13: 11000000 INFO @ Sat, 15 Jan 2022 21:15:17: 8000000 INFO @ Sat, 15 Jan 2022 21:15:22: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:15:22: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:15:22: #1 total tags in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:15:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:22: #1 tags after filtering in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:15:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:29: 9000000 INFO @ Sat, 15 Jan 2022 21:15:42: 10000000 INFO @ Sat, 15 Jan 2022 21:15:54: 11000000 INFO @ Sat, 15 Jan 2022 21:16:02: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:16:02: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:16:02: #1 total tags in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:16:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:02: #1 tags after filtering in treatment: 11702568 INFO @ Sat, 15 Jan 2022 21:16:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108761/SRX4108761.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling