Job ID = 14521271 SRX = SRX4108760 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25907504 spots for SRR7192189/SRR7192189.sra Written 25907504 spots for SRR7192189/SRR7192189.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:29 25907504 reads; of these: 25907504 (100.00%) were unpaired; of these: 4234554 (16.34%) aligned 0 times 19290434 (74.46%) aligned exactly 1 time 2382516 (9.20%) aligned >1 times 83.66% overall alignment rate Time searching: 00:15:29 Overall time: 00:15:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10575094 / 21672950 = 0.4879 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:21: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:36: 2000000 INFO @ Sat, 15 Jan 2022 21:13:51: 3000000 INFO @ Sat, 15 Jan 2022 21:13:53: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:07: 4000000 INFO @ Sat, 15 Jan 2022 21:14:12: 2000000 INFO @ Sat, 15 Jan 2022 21:14:22: 5000000 INFO @ Sat, 15 Jan 2022 21:14:24: 1000000 INFO @ Sat, 15 Jan 2022 21:14:28: 3000000 INFO @ Sat, 15 Jan 2022 21:14:38: 6000000 INFO @ Sat, 15 Jan 2022 21:14:42: 4000000 INFO @ Sat, 15 Jan 2022 21:14:45: 2000000 INFO @ Sat, 15 Jan 2022 21:14:54: 7000000 INFO @ Sat, 15 Jan 2022 21:14:57: 5000000 INFO @ Sat, 15 Jan 2022 21:15:05: 3000000 INFO @ Sat, 15 Jan 2022 21:15:10: 8000000 INFO @ Sat, 15 Jan 2022 21:15:12: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:15:25: 9000000 INFO @ Sat, 15 Jan 2022 21:15:27: 4000000 INFO @ Sat, 15 Jan 2022 21:15:27: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:15:41: 10000000 INFO @ Sat, 15 Jan 2022 21:15:42: 8000000 INFO @ Sat, 15 Jan 2022 21:15:48: 5000000 INFO @ Sat, 15 Jan 2022 21:15:56: 9000000 INFO @ Sat, 15 Jan 2022 21:15:57: 11000000 INFO @ Sat, 15 Jan 2022 21:15:58: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:15:58: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:15:58: #1 total tags in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:15:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:58: #1 tags after filtering in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:15:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:09: 6000000 INFO @ Sat, 15 Jan 2022 21:16:10: 10000000 INFO @ Sat, 15 Jan 2022 21:16:24: 11000000 INFO @ Sat, 15 Jan 2022 21:16:26: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:16:26: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:16:26: #1 total tags in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:16:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:26: #1 tags after filtering in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:16:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:29: 7000000 INFO @ Sat, 15 Jan 2022 21:16:48: 8000000 INFO @ Sat, 15 Jan 2022 21:17:07: 9000000 INFO @ Sat, 15 Jan 2022 21:17:27: 10000000 INFO @ Sat, 15 Jan 2022 21:17:46: 11000000 INFO @ Sat, 15 Jan 2022 21:17:47: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:17:47: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:17:47: #1 total tags in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:17:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:17:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:17:47: #1 tags after filtering in treatment: 11097856 INFO @ Sat, 15 Jan 2022 21:17:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:17:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:17:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:17:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:17:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:17:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:17:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108760/SRX4108760.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling