Job ID = 14521242 SRX = SRX4108758 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25387997 spots for SRR7192187/SRR7192187.sra Written 25387997 spots for SRR7192187/SRR7192187.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:51 25387997 reads; of these: 25387997 (100.00%) were unpaired; of these: 4246536 (16.73%) aligned 0 times 18807015 (74.08%) aligned exactly 1 time 2334446 (9.20%) aligned >1 times 83.27% overall alignment rate Time searching: 00:15:52 Overall time: 00:15:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10130278 / 21141461 = 0.4792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:08:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:08:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:08:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:08:56: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:10: 2000000 INFO @ Sat, 15 Jan 2022 21:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:25: 3000000 INFO @ Sat, 15 Jan 2022 21:09:29: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:09:40: 4000000 INFO @ Sat, 15 Jan 2022 21:09:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:09:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:09:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:09:48: 2000000 INFO @ Sat, 15 Jan 2022 21:09:55: 5000000 INFO @ Sat, 15 Jan 2022 21:09:59: 1000000 INFO @ Sat, 15 Jan 2022 21:10:07: 3000000 INFO @ Sat, 15 Jan 2022 21:10:11: 6000000 INFO @ Sat, 15 Jan 2022 21:10:19: 2000000 INFO @ Sat, 15 Jan 2022 21:10:26: 4000000 INFO @ Sat, 15 Jan 2022 21:10:27: 7000000 INFO @ Sat, 15 Jan 2022 21:10:37: 3000000 INFO @ Sat, 15 Jan 2022 21:10:45: 8000000 INFO @ Sat, 15 Jan 2022 21:10:45: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:10:55: 4000000 INFO @ Sat, 15 Jan 2022 21:11:00: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:11:05: 6000000 INFO @ Sat, 15 Jan 2022 21:11:14: 5000000 INFO @ Sat, 15 Jan 2022 21:11:15: 10000000 INFO @ Sat, 15 Jan 2022 21:11:24: 7000000 INFO @ Sat, 15 Jan 2022 21:11:30: 11000000 INFO @ Sat, 15 Jan 2022 21:11:30: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:11:30: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:11:30: #1 total tags in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:11:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:31: #1 tags after filtering in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:11:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:11:31: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:33: 6000000 INFO @ Sat, 15 Jan 2022 21:11:43: 8000000 INFO @ Sat, 15 Jan 2022 21:11:51: 7000000 INFO @ Sat, 15 Jan 2022 21:12:01: 9000000 INFO @ Sat, 15 Jan 2022 21:12:09: 8000000 INFO @ Sat, 15 Jan 2022 21:12:20: 10000000 INFO @ Sat, 15 Jan 2022 21:12:27: 9000000 INFO @ Sat, 15 Jan 2022 21:12:38: 11000000 INFO @ Sat, 15 Jan 2022 21:12:38: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:12:38: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:12:38: #1 total tags in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:12:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:38: #1 tags after filtering in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:12:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:12:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:44: 10000000 INFO @ Sat, 15 Jan 2022 21:12:59: 11000000 INFO @ Sat, 15 Jan 2022 21:13:00: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:13:00: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:13:00: #1 total tags in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:13:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:00: #1 tags after filtering in treatment: 11011183 INFO @ Sat, 15 Jan 2022 21:13:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:13:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108758/SRX4108758.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling