Job ID = 14521241 SRX = SRX4108757 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27122624 spots for SRR7192186/SRR7192186.sra Written 27122624 spots for SRR7192186/SRR7192186.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 27122624 reads; of these: 27122624 (100.00%) were unpaired; of these: 6430264 (23.71%) aligned 0 times 18251819 (67.29%) aligned exactly 1 time 2440541 (9.00%) aligned >1 times 76.29% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8598721 / 20692360 = 0.4156 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:01:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:01:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:01:29: 1000000 INFO @ Sat, 15 Jan 2022 21:01:37: 2000000 INFO @ Sat, 15 Jan 2022 21:01:45: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:01:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:01:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:01:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:01:52: 4000000 INFO @ Sat, 15 Jan 2022 21:02:00: 1000000 INFO @ Sat, 15 Jan 2022 21:02:00: 5000000 INFO @ Sat, 15 Jan 2022 21:02:07: 2000000 INFO @ Sat, 15 Jan 2022 21:02:08: 6000000 INFO @ Sat, 15 Jan 2022 21:02:15: 3000000 INFO @ Sat, 15 Jan 2022 21:02:16: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:23: 4000000 INFO @ Sat, 15 Jan 2022 21:02:23: 8000000 INFO @ Sat, 15 Jan 2022 21:02:30: 1000000 INFO @ Sat, 15 Jan 2022 21:02:31: 9000000 INFO @ Sat, 15 Jan 2022 21:02:31: 5000000 INFO @ Sat, 15 Jan 2022 21:02:38: 2000000 INFO @ Sat, 15 Jan 2022 21:02:39: 10000000 INFO @ Sat, 15 Jan 2022 21:02:39: 6000000 INFO @ Sat, 15 Jan 2022 21:02:46: 3000000 INFO @ Sat, 15 Jan 2022 21:02:48: 7000000 INFO @ Sat, 15 Jan 2022 21:02:48: 11000000 INFO @ Sat, 15 Jan 2022 21:02:54: 4000000 INFO @ Sat, 15 Jan 2022 21:02:55: 8000000 INFO @ Sat, 15 Jan 2022 21:02:56: 12000000 INFO @ Sat, 15 Jan 2022 21:02:57: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:02:57: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:02:57: #1 total tags in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:02:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:02:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:02:57: #1 tags after filtering in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:02:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:02:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:02:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:02:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:02:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:02:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:02:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:03:03: 5000000 INFO @ Sat, 15 Jan 2022 21:03:04: 9000000 INFO @ Sat, 15 Jan 2022 21:03:11: 6000000 INFO @ Sat, 15 Jan 2022 21:03:12: 10000000 INFO @ Sat, 15 Jan 2022 21:03:19: 7000000 INFO @ Sat, 15 Jan 2022 21:03:21: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:03:27: 8000000 INFO @ Sat, 15 Jan 2022 21:03:29: 12000000 INFO @ Sat, 15 Jan 2022 21:03:30: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:03:30: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:03:30: #1 total tags in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:03:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:30: #1 tags after filtering in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:03:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:03:30: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:03:35: 9000000 INFO @ Sat, 15 Jan 2022 21:03:43: 10000000 INFO @ Sat, 15 Jan 2022 21:03:51: 11000000 INFO @ Sat, 15 Jan 2022 21:03:59: 12000000 INFO @ Sat, 15 Jan 2022 21:03:59: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:03:59: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:03:59: #1 total tags in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:03:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:04:00: #1 tags after filtering in treatment: 12093639 INFO @ Sat, 15 Jan 2022 21:04:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:04:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:04:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:04:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:04:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:04:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:04:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108757/SRX4108757.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling