Job ID = 14521240 SRX = SRX4108756 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42108279 spots for SRR7192185/SRR7192185.sra Written 42108279 spots for SRR7192185/SRR7192185.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:32 42108279 reads; of these: 42108279 (100.00%) were unpaired; of these: 16392382 (38.93%) aligned 0 times 22705741 (53.92%) aligned exactly 1 time 3010156 (7.15%) aligned >1 times 61.07% overall alignment rate Time searching: 00:16:32 Overall time: 00:16:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14197171 / 25715897 = 0.5521 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:14: 1000000 INFO @ Sat, 15 Jan 2022 21:12:23: 2000000 INFO @ Sat, 15 Jan 2022 21:12:31: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:40: 4000000 INFO @ Sat, 15 Jan 2022 21:12:43: 1000000 INFO @ Sat, 15 Jan 2022 21:12:49: 5000000 INFO @ Sat, 15 Jan 2022 21:12:51: 2000000 INFO @ Sat, 15 Jan 2022 21:12:58: 6000000 INFO @ Sat, 15 Jan 2022 21:12:59: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:07: 4000000 INFO @ Sat, 15 Jan 2022 21:13:07: 7000000 INFO @ Sat, 15 Jan 2022 21:13:15: 5000000 INFO @ Sat, 15 Jan 2022 21:13:15: 1000000 INFO @ Sat, 15 Jan 2022 21:13:16: 8000000 INFO @ Sat, 15 Jan 2022 21:13:22: 6000000 INFO @ Sat, 15 Jan 2022 21:13:24: 2000000 INFO @ Sat, 15 Jan 2022 21:13:25: 9000000 INFO @ Sat, 15 Jan 2022 21:13:30: 7000000 INFO @ Sat, 15 Jan 2022 21:13:33: 3000000 INFO @ Sat, 15 Jan 2022 21:13:34: 10000000 INFO @ Sat, 15 Jan 2022 21:13:38: 8000000 INFO @ Sat, 15 Jan 2022 21:13:43: 4000000 INFO @ Sat, 15 Jan 2022 21:13:43: 11000000 INFO @ Sat, 15 Jan 2022 21:13:46: 9000000 INFO @ Sat, 15 Jan 2022 21:13:47: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:13:47: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:13:47: #1 total tags in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:13:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:48: #1 tags after filtering in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:13:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:13:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:13:51: 5000000 INFO @ Sat, 15 Jan 2022 21:13:54: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:14:00: 6000000 INFO @ Sat, 15 Jan 2022 21:14:02: 11000000 INFO @ Sat, 15 Jan 2022 21:14:06: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:14:06: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:14:06: #1 total tags in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:14:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:06: #1 tags after filtering in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:14:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:14:09: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:14:18: 8000000 INFO @ Sat, 15 Jan 2022 21:14:26: 9000000 INFO @ Sat, 15 Jan 2022 21:14:35: 10000000 INFO @ Sat, 15 Jan 2022 21:14:43: 11000000 INFO @ Sat, 15 Jan 2022 21:14:48: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:14:48: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:14:48: #1 total tags in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:14:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:48: #1 tags after filtering in treatment: 11518726 INFO @ Sat, 15 Jan 2022 21:14:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108756/SRX4108756.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling