Job ID = 14521239 SRX = SRX4108755 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36264400 spots for SRR7192184/SRR7192184.sra Written 36264400 spots for SRR7192184/SRR7192184.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:06 36264400 reads; of these: 36264400 (100.00%) were unpaired; of these: 13148870 (36.26%) aligned 0 times 20419180 (56.31%) aligned exactly 1 time 2696350 (7.44%) aligned >1 times 63.74% overall alignment rate Time searching: 00:14:06 Overall time: 00:14:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12385127 / 23115530 = 0.5358 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:06:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:06:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:06:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:06:44: 1000000 INFO @ Sat, 15 Jan 2022 21:06:54: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:07:04: 3000000 INFO @ Sat, 15 Jan 2022 21:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:07:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:07:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:14: 4000000 INFO @ Sat, 15 Jan 2022 21:07:15: 1000000 INFO @ Sat, 15 Jan 2022 21:07:25: 5000000 INFO @ Sat, 15 Jan 2022 21:07:26: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:07:35: 6000000 INFO @ Sat, 15 Jan 2022 21:07:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:07:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:07:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:07:36: 3000000 INFO @ Sat, 15 Jan 2022 21:07:45: 1000000 INFO @ Sat, 15 Jan 2022 21:07:45: 7000000 INFO @ Sat, 15 Jan 2022 21:07:47: 4000000 INFO @ Sat, 15 Jan 2022 21:07:56: 8000000 INFO @ Sat, 15 Jan 2022 21:07:56: 2000000 INFO @ Sat, 15 Jan 2022 21:07:58: 5000000 INFO @ Sat, 15 Jan 2022 21:08:06: 9000000 INFO @ Sat, 15 Jan 2022 21:08:07: 3000000 INFO @ Sat, 15 Jan 2022 21:08:08: 6000000 INFO @ Sat, 15 Jan 2022 21:08:17: 10000000 INFO @ Sat, 15 Jan 2022 21:08:18: 4000000 INFO @ Sat, 15 Jan 2022 21:08:18: 7000000 INFO @ Sat, 15 Jan 2022 21:08:24: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:08:24: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:08:24: #1 total tags in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:08:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:24: #1 tags after filtering in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:08:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:08:24: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:08:28: 5000000 INFO @ Sat, 15 Jan 2022 21:08:29: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:08:38: 6000000 INFO @ Sat, 15 Jan 2022 21:08:39: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:08:49: 7000000 INFO @ Sat, 15 Jan 2022 21:08:50: 10000000 INFO @ Sat, 15 Jan 2022 21:08:57: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:08:57: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:08:57: #1 total tags in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:08:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:08:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:08:57: #1 tags after filtering in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:08:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:08:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:08:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:08:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:08:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:08:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:08:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:09:00: 8000000 INFO @ Sat, 15 Jan 2022 21:09:10: 9000000 INFO @ Sat, 15 Jan 2022 21:09:20: 10000000 INFO @ Sat, 15 Jan 2022 21:09:27: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:09:27: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:09:27: #1 total tags in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:09:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:09:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:09:27: #1 tags after filtering in treatment: 10730403 INFO @ Sat, 15 Jan 2022 21:09:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:09:27: #1 finished! INFO @ Sat, 15 Jan 2022 21:09:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:09:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:09:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:09:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:09:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108755/SRX4108755.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling