Job ID = 14521238 SRX = SRX4108754 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27587150 spots for SRR7192183/SRR7192183.sra Written 27587150 spots for SRR7192183/SRR7192183.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:18 27587150 reads; of these: 27587150 (100.00%) were unpaired; of these: 4141154 (15.01%) aligned 0 times 20711636 (75.08%) aligned exactly 1 time 2734360 (9.91%) aligned >1 times 84.99% overall alignment rate Time searching: 00:10:18 Overall time: 00:10:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11924411 / 23445996 = 0.5086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:01:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:01:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:01:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:01:39: 1000000 INFO @ Sat, 15 Jan 2022 21:01:48: 2000000 INFO @ Sat, 15 Jan 2022 21:01:55: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:03: 4000000 INFO @ Sat, 15 Jan 2022 21:02:09: 1000000 INFO @ Sat, 15 Jan 2022 21:02:11: 5000000 INFO @ Sat, 15 Jan 2022 21:02:16: 2000000 INFO @ Sat, 15 Jan 2022 21:02:19: 6000000 INFO @ Sat, 15 Jan 2022 21:02:25: 3000000 INFO @ Sat, 15 Jan 2022 21:02:28: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:33: 4000000 INFO @ Sat, 15 Jan 2022 21:02:38: 8000000 INFO @ Sat, 15 Jan 2022 21:02:39: 1000000 INFO @ Sat, 15 Jan 2022 21:02:42: 5000000 INFO @ Sat, 15 Jan 2022 21:02:47: 9000000 INFO @ Sat, 15 Jan 2022 21:02:48: 2000000 INFO @ Sat, 15 Jan 2022 21:02:49: 6000000 INFO @ Sat, 15 Jan 2022 21:02:56: 3000000 INFO @ Sat, 15 Jan 2022 21:02:56: 10000000 INFO @ Sat, 15 Jan 2022 21:02:57: 7000000 INFO @ Sat, 15 Jan 2022 21:03:03: 4000000 INFO @ Sat, 15 Jan 2022 21:03:05: 11000000 INFO @ Sat, 15 Jan 2022 21:03:05: 8000000 INFO @ Sat, 15 Jan 2022 21:03:10: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:03:10: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:03:10: #1 total tags in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:03:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:10: #1 tags after filtering in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:03:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:03:10: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 21:03:11: 5000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:03:13: 9000000 INFO @ Sat, 15 Jan 2022 21:03:19: 6000000 INFO @ Sat, 15 Jan 2022 21:03:20: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:03:28: 11000000 INFO @ Sat, 15 Jan 2022 21:03:28: 7000000 INFO @ Sat, 15 Jan 2022 21:03:32: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:03:32: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:03:32: #1 total tags in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:03:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:03:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:03:32: #1 tags after filtering in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:03:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:03:32: #1 finished! INFO @ Sat, 15 Jan 2022 21:03:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:03:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:03:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:03:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:03:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:03:37: 8000000 INFO @ Sat, 15 Jan 2022 21:03:46: 9000000 INFO @ Sat, 15 Jan 2022 21:03:55: 10000000 INFO @ Sat, 15 Jan 2022 21:04:03: 11000000 INFO @ Sat, 15 Jan 2022 21:04:07: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:04:07: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:04:07: #1 total tags in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:04:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:04:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:04:08: #1 tags after filtering in treatment: 11521585 INFO @ Sat, 15 Jan 2022 21:04:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:04:08: #1 finished! INFO @ Sat, 15 Jan 2022 21:04:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:04:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:04:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:04:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:04:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108754/SRX4108754.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling