Job ID = 14521237 SRX = SRX4108753 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 28853323 spots for SRR7192182/SRR7192182.sra Written 28853323 spots for SRR7192182/SRR7192182.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:18 28853323 reads; of these: 28853323 (100.00%) were unpaired; of these: 6764705 (23.45%) aligned 0 times 19392058 (67.21%) aligned exactly 1 time 2696560 (9.35%) aligned >1 times 76.55% overall alignment rate Time searching: 00:11:18 Overall time: 00:11:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9607524 / 22088618 = 0.4350 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:26: 1000000 INFO @ Sat, 15 Jan 2022 21:02:35: 2000000 INFO @ Sat, 15 Jan 2022 21:02:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:02:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:02:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:02:54: 4000000 INFO @ Sat, 15 Jan 2022 21:02:56: 1000000 INFO @ Sat, 15 Jan 2022 21:03:05: 5000000 INFO @ Sat, 15 Jan 2022 21:03:05: 2000000 INFO @ Sat, 15 Jan 2022 21:03:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:03:15: 6000000 INFO @ Sat, 15 Jan 2022 21:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:03:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:03:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:03:24: 4000000 INFO @ Sat, 15 Jan 2022 21:03:25: 7000000 INFO @ Sat, 15 Jan 2022 21:03:27: 1000000 INFO @ Sat, 15 Jan 2022 21:03:33: 5000000 INFO @ Sat, 15 Jan 2022 21:03:36: 8000000 INFO @ Sat, 15 Jan 2022 21:03:37: 2000000 INFO @ Sat, 15 Jan 2022 21:03:43: 6000000 INFO @ Sat, 15 Jan 2022 21:03:46: 9000000 INFO @ Sat, 15 Jan 2022 21:03:48: 3000000 INFO @ Sat, 15 Jan 2022 21:03:52: 7000000 INFO @ Sat, 15 Jan 2022 21:03:56: 10000000 INFO @ Sat, 15 Jan 2022 21:03:58: 4000000 INFO @ Sat, 15 Jan 2022 21:04:01: 8000000 INFO @ Sat, 15 Jan 2022 21:04:07: 11000000 INFO @ Sat, 15 Jan 2022 21:04:08: 5000000 INFO @ Sat, 15 Jan 2022 21:04:11: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:04:17: 12000000 INFO @ Sat, 15 Jan 2022 21:04:19: 6000000 INFO @ Sat, 15 Jan 2022 21:04:20: 10000000 INFO @ Sat, 15 Jan 2022 21:04:22: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:04:22: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:04:22: #1 total tags in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:04:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:04:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:04:22: #1 tags after filtering in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:04:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:04:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:04:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:04:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:04:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:04:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:04:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:04:29: 7000000 INFO @ Sat, 15 Jan 2022 21:04:29: 11000000 INFO @ Sat, 15 Jan 2022 21:04:39: 12000000 INFO @ Sat, 15 Jan 2022 21:04:40: 8000000 INFO @ Sat, 15 Jan 2022 21:04:43: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:04:43: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:04:43: #1 total tags in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:04:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:04:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:04:43: #1 tags after filtering in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:04:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:04:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:04:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:04:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:04:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:04:49: 9000000 INFO @ Sat, 15 Jan 2022 21:04:59: 10000000 INFO @ Sat, 15 Jan 2022 21:05:08: 11000000 INFO @ Sat, 15 Jan 2022 21:05:18: 12000000 INFO @ Sat, 15 Jan 2022 21:05:22: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:05:22: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:05:22: #1 total tags in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:05:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:05:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:05:23: #1 tags after filtering in treatment: 12481094 INFO @ Sat, 15 Jan 2022 21:05:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:05:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:05:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:05:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:05:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:05:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:05:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108753/SRX4108753.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling