Job ID = 14521236 SRX = SRX4108752 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42198476 spots for SRR7192181/SRR7192181.sra Written 42198476 spots for SRR7192181/SRR7192181.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:35 42198476 reads; of these: 42198476 (100.00%) were unpaired; of these: 18116529 (42.93%) aligned 0 times 20992685 (49.75%) aligned exactly 1 time 3089262 (7.32%) aligned >1 times 57.07% overall alignment rate Time searching: 00:24:35 Overall time: 00:24:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12586324 / 24081947 = 0.5226 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:31:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:31:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:31:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:31:51: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:32:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:32:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:32:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:32:05: 2000000 INFO @ Sat, 15 Jan 2022 21:32:21: 1000000 INFO @ Sat, 15 Jan 2022 21:32:22: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:32:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:32:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:32:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:32:39: 2000000 INFO @ Sat, 15 Jan 2022 21:32:39: 4000000 INFO @ Sat, 15 Jan 2022 21:32:46: 1000000 INFO @ Sat, 15 Jan 2022 21:32:56: 3000000 INFO @ Sat, 15 Jan 2022 21:32:56: 5000000 INFO @ Sat, 15 Jan 2022 21:32:59: 2000000 INFO @ Sat, 15 Jan 2022 21:33:12: 3000000 INFO @ Sat, 15 Jan 2022 21:33:13: 6000000 INFO @ Sat, 15 Jan 2022 21:33:14: 4000000 INFO @ Sat, 15 Jan 2022 21:33:25: 4000000 INFO @ Sat, 15 Jan 2022 21:33:31: 7000000 INFO @ Sat, 15 Jan 2022 21:33:31: 5000000 INFO @ Sat, 15 Jan 2022 21:33:37: 5000000 INFO @ Sat, 15 Jan 2022 21:33:48: 6000000 INFO @ Sat, 15 Jan 2022 21:33:49: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:33:50: 6000000 INFO @ Sat, 15 Jan 2022 21:34:03: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:34:06: 7000000 INFO @ Sat, 15 Jan 2022 21:34:06: 9000000 INFO @ Sat, 15 Jan 2022 21:34:16: 8000000 INFO @ Sat, 15 Jan 2022 21:34:22: 10000000 INFO @ Sat, 15 Jan 2022 21:34:23: 8000000 INFO @ Sat, 15 Jan 2022 21:34:28: 9000000 INFO @ Sat, 15 Jan 2022 21:34:38: 11000000 INFO @ Sat, 15 Jan 2022 21:34:41: 9000000 INFO @ Sat, 15 Jan 2022 21:34:41: 10000000 INFO @ Sat, 15 Jan 2022 21:34:46: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:34:46: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:34:46: #1 total tags in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:34:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:34:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:34:47: #1 tags after filtering in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:34:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:34:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:34:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:34:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:34:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:34:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:34:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:34:53: 11000000 INFO @ Sat, 15 Jan 2022 21:34:56: 10000000 INFO @ Sat, 15 Jan 2022 21:35:00: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:35:00: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:35:00: #1 total tags in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:35:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:00: #1 tags after filtering in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:35:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:35:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:35:11: 11000000 INFO @ Sat, 15 Jan 2022 21:35:18: #1 tag size is determined as 150 bps INFO @ Sat, 15 Jan 2022 21:35:18: #1 tag size = 150 INFO @ Sat, 15 Jan 2022 21:35:18: #1 total tags in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:35:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:18: #1 tags after filtering in treatment: 11495623 INFO @ Sat, 15 Jan 2022 21:35:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:35:18: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4108752/SRX4108752.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling