Job ID = 11634686 sra ファイルのダウンロード中... Completed: 572824K bytes transferred in 8 seconds (553142K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 26038251 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092966/SRR7175394.sra Written 26038251 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092966/SRR7175394.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 26038251 reads; of these: 26038251 (100.00%) were unpaired; of these: 1611336 (6.19%) aligned 0 times 19301971 (74.13%) aligned exactly 1 time 5124944 (19.68%) aligned >1 times 93.81% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11176171 / 24426915 = 0.4575 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:37:18: # Command line: callpeak -t SRX4092966.bam -f BAM -g 12100000 -n SRX4092966.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4092966.05 # format = BAM # ChIP-seq file = ['SRX4092966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:37:18: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:37:18: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:37:18: # Command line: callpeak -t SRX4092966.bam -f BAM -g 12100000 -n SRX4092966.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4092966.10 # format = BAM # ChIP-seq file = ['SRX4092966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:37:18: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:37:18: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:37:18: # Command line: callpeak -t SRX4092966.bam -f BAM -g 12100000 -n SRX4092966.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4092966.20 # format = BAM # ChIP-seq file = ['SRX4092966.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:37:18: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:37:18: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:37:24: 1000000 INFO @ Fri, 15 Feb 2019 10:37:24: 1000000 INFO @ Fri, 15 Feb 2019 10:37:24: 1000000 INFO @ Fri, 15 Feb 2019 10:37:30: 2000000 INFO @ Fri, 15 Feb 2019 10:37:30: 2000000 INFO @ Fri, 15 Feb 2019 10:37:31: 2000000 INFO @ Fri, 15 Feb 2019 10:37:36: 3000000 INFO @ Fri, 15 Feb 2019 10:37:36: 3000000 INFO @ Fri, 15 Feb 2019 10:37:37: 3000000 INFO @ Fri, 15 Feb 2019 10:37:42: 4000000 INFO @ Fri, 15 Feb 2019 10:37:42: 4000000 INFO @ Fri, 15 Feb 2019 10:37:43: 4000000 INFO @ Fri, 15 Feb 2019 10:37:48: 5000000 INFO @ Fri, 15 Feb 2019 10:37:49: 5000000 INFO @ Fri, 15 Feb 2019 10:37:49: 5000000 INFO @ Fri, 15 Feb 2019 10:37:54: 6000000 INFO @ Fri, 15 Feb 2019 10:37:55: 6000000 INFO @ Fri, 15 Feb 2019 10:37:55: 6000000 INFO @ Fri, 15 Feb 2019 10:38:00: 7000000 INFO @ Fri, 15 Feb 2019 10:38:01: 7000000 INFO @ Fri, 15 Feb 2019 10:38:02: 7000000 INFO @ Fri, 15 Feb 2019 10:38:06: 8000000 INFO @ Fri, 15 Feb 2019 10:38:07: 8000000 INFO @ Fri, 15 Feb 2019 10:38:08: 8000000 INFO @ Fri, 15 Feb 2019 10:38:12: 9000000 INFO @ Fri, 15 Feb 2019 10:38:13: 9000000 INFO @ Fri, 15 Feb 2019 10:38:15: 9000000 INFO @ Fri, 15 Feb 2019 10:38:18: 10000000 INFO @ Fri, 15 Feb 2019 10:38:20: 10000000 INFO @ Fri, 15 Feb 2019 10:38:21: 10000000 INFO @ Fri, 15 Feb 2019 10:38:24: 11000000 INFO @ Fri, 15 Feb 2019 10:38:26: 11000000 INFO @ Fri, 15 Feb 2019 10:38:28: 11000000 INFO @ Fri, 15 Feb 2019 10:38:30: 12000000 INFO @ Fri, 15 Feb 2019 10:38:32: 12000000 INFO @ Fri, 15 Feb 2019 10:38:34: 12000000 INFO @ Fri, 15 Feb 2019 10:38:36: 13000000 INFO @ Fri, 15 Feb 2019 10:38:38: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:38: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:38: #1 total tags in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:38: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:38: #1 tags after filtering in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:38: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:38: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:39: 13000000 INFO @ Fri, 15 Feb 2019 10:38:39: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:39: Process for pairing-model is terminated! cat: SRX4092966.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092966.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:38:40: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:40: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:40: #1 total tags in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:40: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:40: 13000000 INFO @ Fri, 15 Feb 2019 10:38:40: #1 tags after filtering in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:40: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:40: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:41: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:41: Process for pairing-model is terminated! cat: SRX4092966.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092966.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:38:42: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:38:42: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:38:42: #1 total tags in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:42: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:38:42: #1 tags after filtering in treatment: 13250744 INFO @ Fri, 15 Feb 2019 10:38:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:38:42: #1 finished! INFO @ Fri, 15 Feb 2019 10:38:42: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:38:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:38:43: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:38:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:38:43: Process for pairing-model is terminated! cat: SRX4092966.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092966.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092966.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。