Job ID = 11634666 sra ファイルのダウンロード中... Completed: 767705K bytes transferred in 8 seconds (770424K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 36323908 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092946/SRR7175374.sra Written 36323908 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4092946/SRR7175374.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:22 36323908 reads; of these: 36323908 (100.00%) were unpaired; of these: 1872641 (5.16%) aligned 0 times 27403255 (75.44%) aligned exactly 1 time 7048012 (19.40%) aligned >1 times 94.84% overall alignment rate Time searching: 00:06:22 Overall time: 00:06:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18319235 / 34451267 = 0.5317 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:46:39: # Command line: callpeak -t SRX4092946.bam -f BAM -g 12100000 -n SRX4092946.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4092946.05 # format = BAM # ChIP-seq file = ['SRX4092946.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:46:39: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:46:39: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:46:39: # Command line: callpeak -t SRX4092946.bam -f BAM -g 12100000 -n SRX4092946.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4092946.10 # format = BAM # ChIP-seq file = ['SRX4092946.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:46:39: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:46:39: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:46:39: # Command line: callpeak -t SRX4092946.bam -f BAM -g 12100000 -n SRX4092946.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4092946.20 # format = BAM # ChIP-seq file = ['SRX4092946.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:46:39: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:46:39: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:46:46: 1000000 INFO @ Fri, 15 Feb 2019 10:46:47: 1000000 INFO @ Fri, 15 Feb 2019 10:46:47: 1000000 INFO @ Fri, 15 Feb 2019 10:46:53: 2000000 INFO @ Fri, 15 Feb 2019 10:46:55: 2000000 INFO @ Fri, 15 Feb 2019 10:46:55: 2000000 INFO @ Fri, 15 Feb 2019 10:46:59: 3000000 INFO @ Fri, 15 Feb 2019 10:47:03: 3000000 INFO @ Fri, 15 Feb 2019 10:47:03: 3000000 INFO @ Fri, 15 Feb 2019 10:47:05: 4000000 INFO @ Fri, 15 Feb 2019 10:47:09: 4000000 INFO @ Fri, 15 Feb 2019 10:47:09: 4000000 INFO @ Fri, 15 Feb 2019 10:47:11: 5000000 INFO @ Fri, 15 Feb 2019 10:47:15: 5000000 INFO @ Fri, 15 Feb 2019 10:47:16: 5000000 INFO @ Fri, 15 Feb 2019 10:47:17: 6000000 INFO @ Fri, 15 Feb 2019 10:47:22: 6000000 INFO @ Fri, 15 Feb 2019 10:47:22: 6000000 INFO @ Fri, 15 Feb 2019 10:47:23: 7000000 INFO @ Fri, 15 Feb 2019 10:47:28: 7000000 INFO @ Fri, 15 Feb 2019 10:47:28: 7000000 INFO @ Fri, 15 Feb 2019 10:47:30: 8000000 INFO @ Fri, 15 Feb 2019 10:47:35: 8000000 INFO @ Fri, 15 Feb 2019 10:47:35: 8000000 INFO @ Fri, 15 Feb 2019 10:47:36: 9000000 INFO @ Fri, 15 Feb 2019 10:47:41: 9000000 INFO @ Fri, 15 Feb 2019 10:47:42: 9000000 INFO @ Fri, 15 Feb 2019 10:47:42: 10000000 INFO @ Fri, 15 Feb 2019 10:47:48: 11000000 INFO @ Fri, 15 Feb 2019 10:47:48: 10000000 INFO @ Fri, 15 Feb 2019 10:47:48: 10000000 INFO @ Fri, 15 Feb 2019 10:47:54: 12000000 INFO @ Fri, 15 Feb 2019 10:47:55: 11000000 INFO @ Fri, 15 Feb 2019 10:47:55: 11000000 INFO @ Fri, 15 Feb 2019 10:48:00: 13000000 INFO @ Fri, 15 Feb 2019 10:48:01: 12000000 INFO @ Fri, 15 Feb 2019 10:48:02: 12000000 INFO @ Fri, 15 Feb 2019 10:48:06: 14000000 INFO @ Fri, 15 Feb 2019 10:48:08: 13000000 INFO @ Fri, 15 Feb 2019 10:48:09: 13000000 INFO @ Fri, 15 Feb 2019 10:48:12: 15000000 INFO @ Fri, 15 Feb 2019 10:48:15: 14000000 INFO @ Fri, 15 Feb 2019 10:48:16: 14000000 INFO @ Fri, 15 Feb 2019 10:48:18: 16000000 INFO @ Fri, 15 Feb 2019 10:48:19: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:48:19: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:48:19: #1 total tags in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:19: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:48:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:48:19: #1 tags after filtering in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:48:19: #1 finished! INFO @ Fri, 15 Feb 2019 10:48:19: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:48:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:48:20: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:48:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:48:20: Process for pairing-model is terminated! cat: SRX4092946.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092946.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:48:21: 15000000 INFO @ Fri, 15 Feb 2019 10:48:22: 15000000 INFO @ Fri, 15 Feb 2019 10:48:28: 16000000 INFO @ Fri, 15 Feb 2019 10:48:29: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:48:29: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:48:29: #1 total tags in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:48:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:48:29: 16000000 INFO @ Fri, 15 Feb 2019 10:48:29: #1 tags after filtering in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:48:29: #1 finished! INFO @ Fri, 15 Feb 2019 10:48:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:48:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:48:30: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 10:48:30: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 10:48:30: #1 total tags in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:48:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:48:30: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:48:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:48:30: Process for pairing-model is terminated! cat: SRX4092946.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092946.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:48:30: #1 tags after filtering in treatment: 16132032 INFO @ Fri, 15 Feb 2019 10:48:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:48:30: #1 finished! INFO @ Fri, 15 Feb 2019 10:48:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:48:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:48:31: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:48:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:48:31: Process for pairing-model is terminated! cat: SRX4092946.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4092946.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4092946.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。