Job ID = 11244886 sra ファイルのダウンロード中... Completed: 1069577K bytes transferred in 13 seconds (626467K bits/sec), in 1 file. Completed: 1215599K bytes transferred in 17 seconds (585593K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 23155045 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4065333/SRR7145385.sra Written 23155045 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4065333/SRR7145385.sra Read 26308766 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4065333/SRR7145386.sra Written 26308766 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4065333/SRR7145386.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:41 49463811 reads; of these: 49463811 (100.00%) were paired; of these: 2373249 (4.80%) aligned concordantly 0 times 35822422 (72.42%) aligned concordantly exactly 1 time 11268140 (22.78%) aligned concordantly >1 times ---- 2373249 pairs aligned concordantly 0 times; of these: 639699 (26.95%) aligned discordantly 1 time ---- 1733550 pairs aligned 0 times concordantly or discordantly; of these: 3467100 mates make up the pairs; of these: 2505511 (72.27%) aligned 0 times 308595 (8.90%) aligned exactly 1 time 652994 (18.83%) aligned >1 times 97.47% overall alignment rate Time searching: 00:44:41 Overall time: 00:44:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7472654 / 47535429 = 0.1572 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:46:58: # Command line: callpeak -t SRX4065333.bam -f BAM -g 12100000 -n SRX4065333.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4065333.20 # format = BAM # ChIP-seq file = ['SRX4065333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:46:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:46:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:46:58: # Command line: callpeak -t SRX4065333.bam -f BAM -g 12100000 -n SRX4065333.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4065333.05 # format = BAM # ChIP-seq file = ['SRX4065333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:46:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:46:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:46:58: # Command line: callpeak -t SRX4065333.bam -f BAM -g 12100000 -n SRX4065333.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4065333.10 # format = BAM # ChIP-seq file = ['SRX4065333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:46:58: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:46:58: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:47:05: 1000000 INFO @ Tue, 09 Oct 2018 23:47:05: 1000000 INFO @ Tue, 09 Oct 2018 23:47:06: 1000000 INFO @ Tue, 09 Oct 2018 23:47:12: 2000000 INFO @ Tue, 09 Oct 2018 23:47:12: 2000000 INFO @ Tue, 09 Oct 2018 23:47:13: 2000000 INFO @ Tue, 09 Oct 2018 23:47:19: 3000000 INFO @ Tue, 09 Oct 2018 23:47:19: 3000000 INFO @ Tue, 09 Oct 2018 23:47:20: 3000000 INFO @ Tue, 09 Oct 2018 23:47:26: 4000000 INFO @ Tue, 09 Oct 2018 23:47:26: 4000000 INFO @ Tue, 09 Oct 2018 23:47:27: 4000000 INFO @ Tue, 09 Oct 2018 23:47:33: 5000000 INFO @ Tue, 09 Oct 2018 23:47:33: 5000000 INFO @ Tue, 09 Oct 2018 23:47:34: 5000000 INFO @ Tue, 09 Oct 2018 23:47:40: 6000000 INFO @ Tue, 09 Oct 2018 23:47:40: 6000000 INFO @ Tue, 09 Oct 2018 23:47:40: 6000000 INFO @ Tue, 09 Oct 2018 23:47:46: 7000000 INFO @ Tue, 09 Oct 2018 23:47:46: 7000000 INFO @ Tue, 09 Oct 2018 23:47:48: 7000000 INFO @ Tue, 09 Oct 2018 23:47:53: 8000000 INFO @ Tue, 09 Oct 2018 23:47:53: 8000000 INFO @ Tue, 09 Oct 2018 23:47:54: 8000000 INFO @ Tue, 09 Oct 2018 23:48:00: 9000000 INFO @ Tue, 09 Oct 2018 23:48:00: 9000000 INFO @ Tue, 09 Oct 2018 23:48:02: 9000000 INFO @ Tue, 09 Oct 2018 23:48:08: 10000000 INFO @ Tue, 09 Oct 2018 23:48:09: 10000000 INFO @ Tue, 09 Oct 2018 23:48:12: 10000000 INFO @ Tue, 09 Oct 2018 23:48:15: 11000000 INFO @ Tue, 09 Oct 2018 23:48:18: 11000000 INFO @ Tue, 09 Oct 2018 23:48:21: 11000000 INFO @ Tue, 09 Oct 2018 23:48:23: 12000000 INFO @ Tue, 09 Oct 2018 23:48:28: 12000000 INFO @ Tue, 09 Oct 2018 23:48:30: 12000000 INFO @ Tue, 09 Oct 2018 23:48:30: 13000000 INFO @ Tue, 09 Oct 2018 23:48:37: 13000000 INFO @ Tue, 09 Oct 2018 23:48:38: 14000000 INFO @ Tue, 09 Oct 2018 23:48:39: 13000000 INFO @ Tue, 09 Oct 2018 23:48:45: 15000000 INFO @ Tue, 09 Oct 2018 23:48:46: 14000000 INFO @ Tue, 09 Oct 2018 23:48:49: 14000000 INFO @ Tue, 09 Oct 2018 23:48:53: 16000000 INFO @ Tue, 09 Oct 2018 23:48:55: 15000000 INFO @ Tue, 09 Oct 2018 23:48:58: 15000000 INFO @ Tue, 09 Oct 2018 23:49:00: 17000000 INFO @ Tue, 09 Oct 2018 23:49:04: 16000000 INFO @ Tue, 09 Oct 2018 23:49:07: 16000000 INFO @ Tue, 09 Oct 2018 23:49:08: 18000000 INFO @ Tue, 09 Oct 2018 23:49:14: 17000000 INFO @ Tue, 09 Oct 2018 23:49:16: 19000000 INFO @ Tue, 09 Oct 2018 23:49:17: 17000000 INFO @ Tue, 09 Oct 2018 23:49:23: 18000000 INFO @ Tue, 09 Oct 2018 23:49:23: 20000000 INFO @ Tue, 09 Oct 2018 23:49:26: 18000000 INFO @ Tue, 09 Oct 2018 23:49:30: 21000000 INFO @ Tue, 09 Oct 2018 23:49:32: 19000000 INFO @ Tue, 09 Oct 2018 23:49:34: 19000000 INFO @ Tue, 09 Oct 2018 23:49:36: 22000000 INFO @ Tue, 09 Oct 2018 23:49:40: 20000000 INFO @ Tue, 09 Oct 2018 23:49:42: 23000000 INFO @ Tue, 09 Oct 2018 23:49:43: 20000000 INFO @ Tue, 09 Oct 2018 23:49:48: 21000000 INFO @ Tue, 09 Oct 2018 23:49:48: 24000000 INFO @ Tue, 09 Oct 2018 23:49:50: 21000000 INFO @ Tue, 09 Oct 2018 23:49:54: 25000000 INFO @ Tue, 09 Oct 2018 23:49:56: 22000000 INFO @ Tue, 09 Oct 2018 23:49:58: 22000000 INFO @ Tue, 09 Oct 2018 23:50:00: 26000000 INFO @ Tue, 09 Oct 2018 23:50:03: 23000000 INFO @ Tue, 09 Oct 2018 23:50:06: 23000000 INFO @ Tue, 09 Oct 2018 23:50:06: 27000000 INFO @ Tue, 09 Oct 2018 23:50:11: 24000000 INFO @ Tue, 09 Oct 2018 23:50:12: 28000000 INFO @ Tue, 09 Oct 2018 23:50:14: 24000000 INFO @ Tue, 09 Oct 2018 23:50:18: 29000000 INFO @ Tue, 09 Oct 2018 23:50:19: 25000000 INFO @ Tue, 09 Oct 2018 23:50:21: 25000000 INFO @ Tue, 09 Oct 2018 23:50:25: 30000000 INFO @ Tue, 09 Oct 2018 23:50:28: 26000000 INFO @ Tue, 09 Oct 2018 23:50:30: 26000000 INFO @ Tue, 09 Oct 2018 23:50:33: 31000000 INFO @ Tue, 09 Oct 2018 23:50:36: 27000000 INFO @ Tue, 09 Oct 2018 23:50:39: 27000000 INFO @ Tue, 09 Oct 2018 23:50:42: 32000000 INFO @ Tue, 09 Oct 2018 23:50:45: 28000000 INFO @ Tue, 09 Oct 2018 23:50:48: 28000000 INFO @ Tue, 09 Oct 2018 23:50:51: 33000000 INFO @ Tue, 09 Oct 2018 23:50:54: 29000000 INFO @ Tue, 09 Oct 2018 23:50:57: 29000000 INFO @ Tue, 09 Oct 2018 23:50:59: 34000000 INFO @ Tue, 09 Oct 2018 23:51:02: 30000000 INFO @ Tue, 09 Oct 2018 23:51:05: 30000000 INFO @ Tue, 09 Oct 2018 23:51:05: 35000000 INFO @ Tue, 09 Oct 2018 23:51:10: 31000000 INFO @ Tue, 09 Oct 2018 23:51:11: 36000000 INFO @ Tue, 09 Oct 2018 23:51:12: 31000000 INFO @ Tue, 09 Oct 2018 23:51:17: 37000000 INFO @ Tue, 09 Oct 2018 23:51:18: 32000000 INFO @ Tue, 09 Oct 2018 23:51:20: 32000000 INFO @ Tue, 09 Oct 2018 23:51:23: 38000000 INFO @ Tue, 09 Oct 2018 23:51:26: 33000000 INFO @ Tue, 09 Oct 2018 23:51:28: 33000000 INFO @ Tue, 09 Oct 2018 23:51:30: 39000000 INFO @ Tue, 09 Oct 2018 23:51:34: 34000000 INFO @ Tue, 09 Oct 2018 23:51:36: 34000000 INFO @ Tue, 09 Oct 2018 23:51:36: 40000000 INFO @ Tue, 09 Oct 2018 23:51:42: 35000000 INFO @ Tue, 09 Oct 2018 23:51:42: 41000000 INFO @ Tue, 09 Oct 2018 23:51:44: 35000000 INFO @ Tue, 09 Oct 2018 23:51:49: 42000000 INFO @ Tue, 09 Oct 2018 23:51:50: 36000000 INFO @ Tue, 09 Oct 2018 23:51:52: 36000000 INFO @ Tue, 09 Oct 2018 23:51:55: 43000000 INFO @ Tue, 09 Oct 2018 23:51:58: 37000000 INFO @ Tue, 09 Oct 2018 23:52:00: 37000000 INFO @ Tue, 09 Oct 2018 23:52:02: 44000000 INFO @ Tue, 09 Oct 2018 23:52:06: 38000000 INFO @ Tue, 09 Oct 2018 23:52:08: 38000000 INFO @ Tue, 09 Oct 2018 23:52:09: 45000000 INFO @ Tue, 09 Oct 2018 23:52:14: 39000000 INFO @ Tue, 09 Oct 2018 23:52:15: 46000000 INFO @ Tue, 09 Oct 2018 23:52:16: 39000000 INFO @ Tue, 09 Oct 2018 23:52:22: 40000000 INFO @ Tue, 09 Oct 2018 23:52:22: 47000000 INFO @ Tue, 09 Oct 2018 23:52:24: 40000000 INFO @ Tue, 09 Oct 2018 23:52:28: 48000000 INFO @ Tue, 09 Oct 2018 23:52:29: 41000000 INFO @ Tue, 09 Oct 2018 23:52:31: 41000000 INFO @ Tue, 09 Oct 2018 23:52:35: 49000000 INFO @ Tue, 09 Oct 2018 23:52:37: 42000000 INFO @ Tue, 09 Oct 2018 23:52:39: 42000000 INFO @ Tue, 09 Oct 2018 23:52:41: 50000000 INFO @ Tue, 09 Oct 2018 23:52:45: 43000000 INFO @ Tue, 09 Oct 2018 23:52:47: 43000000 INFO @ Tue, 09 Oct 2018 23:52:48: 51000000 INFO @ Tue, 09 Oct 2018 23:52:54: 44000000 INFO @ Tue, 09 Oct 2018 23:52:56: 52000000 INFO @ Tue, 09 Oct 2018 23:52:56: 44000000 INFO @ Tue, 09 Oct 2018 23:53:03: 53000000 INFO @ Tue, 09 Oct 2018 23:53:03: 45000000 INFO @ Tue, 09 Oct 2018 23:53:06: 45000000 INFO @ Tue, 09 Oct 2018 23:53:10: 54000000 INFO @ Tue, 09 Oct 2018 23:53:13: 46000000 INFO @ Tue, 09 Oct 2018 23:53:15: 46000000 INFO @ Tue, 09 Oct 2018 23:53:18: 55000000 INFO @ Tue, 09 Oct 2018 23:53:22: 47000000 INFO @ Tue, 09 Oct 2018 23:53:25: 47000000 INFO @ Tue, 09 Oct 2018 23:53:25: 56000000 INFO @ Tue, 09 Oct 2018 23:53:32: 48000000 INFO @ Tue, 09 Oct 2018 23:53:33: 57000000 INFO @ Tue, 09 Oct 2018 23:53:34: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 09 Oct 2018 23:53:40: 58000000 INFO @ Tue, 09 Oct 2018 23:53:41: 49000000 INFO @ Tue, 09 Oct 2018 23:53:43: 49000000 INFO @ Tue, 09 Oct 2018 23:53:48: 59000000 INFO @ Tue, 09 Oct 2018 23:53:51: 50000000 INFO @ Tue, 09 Oct 2018 23:53:53: 50000000 INFO @ Tue, 09 Oct 2018 23:53:55: 60000000 INFO @ Tue, 09 Oct 2018 23:54:00: 51000000 INFO @ Tue, 09 Oct 2018 23:54:02: 51000000 INFO @ Tue, 09 Oct 2018 23:54:02: 61000000 BigWig に変換しました。 INFO @ Tue, 09 Oct 2018 23:54:09: 52000000 INFO @ Tue, 09 Oct 2018 23:54:10: 62000000 INFO @ Tue, 09 Oct 2018 23:54:11: 52000000 INFO @ Tue, 09 Oct 2018 23:54:17: 63000000 INFO @ Tue, 09 Oct 2018 23:54:17: 53000000 INFO @ Tue, 09 Oct 2018 23:54:19: 53000000 INFO @ Tue, 09 Oct 2018 23:54:23: 64000000 INFO @ Tue, 09 Oct 2018 23:54:25: 54000000 INFO @ Tue, 09 Oct 2018 23:54:27: 54000000 INFO @ Tue, 09 Oct 2018 23:54:30: 65000000 INFO @ Tue, 09 Oct 2018 23:54:33: 55000000 INFO @ Tue, 09 Oct 2018 23:54:35: 55000000 INFO @ Tue, 09 Oct 2018 23:54:37: 66000000 INFO @ Tue, 09 Oct 2018 23:54:42: 56000000 INFO @ Tue, 09 Oct 2018 23:54:44: 56000000 INFO @ Tue, 09 Oct 2018 23:54:44: 67000000 INFO @ Tue, 09 Oct 2018 23:54:50: 57000000 INFO @ Tue, 09 Oct 2018 23:54:51: 68000000 INFO @ Tue, 09 Oct 2018 23:54:52: 57000000 INFO @ Tue, 09 Oct 2018 23:54:58: 69000000 INFO @ Tue, 09 Oct 2018 23:54:58: 58000000 INFO @ Tue, 09 Oct 2018 23:55:00: 58000000 INFO @ Tue, 09 Oct 2018 23:55:05: 70000000 INFO @ Tue, 09 Oct 2018 23:55:06: 59000000 INFO @ Tue, 09 Oct 2018 23:55:08: 59000000 INFO @ Tue, 09 Oct 2018 23:55:12: 71000000 INFO @ Tue, 09 Oct 2018 23:55:14: 60000000 INFO @ Tue, 09 Oct 2018 23:55:16: 60000000 INFO @ Tue, 09 Oct 2018 23:55:19: 72000000 INFO @ Tue, 09 Oct 2018 23:55:22: 61000000 INFO @ Tue, 09 Oct 2018 23:55:24: 61000000 INFO @ Tue, 09 Oct 2018 23:55:26: 73000000 INFO @ Tue, 09 Oct 2018 23:55:31: 62000000 INFO @ Tue, 09 Oct 2018 23:55:32: 62000000 INFO @ Tue, 09 Oct 2018 23:55:33: 74000000 INFO @ Tue, 09 Oct 2018 23:55:39: 63000000 INFO @ Tue, 09 Oct 2018 23:55:41: 75000000 INFO @ Tue, 09 Oct 2018 23:55:41: 63000000 INFO @ Tue, 09 Oct 2018 23:55:47: 64000000 INFO @ Tue, 09 Oct 2018 23:55:48: 76000000 INFO @ Tue, 09 Oct 2018 23:55:49: 64000000 INFO @ Tue, 09 Oct 2018 23:55:54: 77000000 INFO @ Tue, 09 Oct 2018 23:55:55: 65000000 INFO @ Tue, 09 Oct 2018 23:55:57: 65000000 INFO @ Tue, 09 Oct 2018 23:56:01: 78000000 INFO @ Tue, 09 Oct 2018 23:56:03: 66000000 INFO @ Tue, 09 Oct 2018 23:56:05: 66000000 INFO @ Tue, 09 Oct 2018 23:56:08: 79000000 INFO @ Tue, 09 Oct 2018 23:56:11: 67000000 INFO @ Tue, 09 Oct 2018 23:56:13: 67000000 INFO @ Tue, 09 Oct 2018 23:56:15: 80000000 INFO @ Tue, 09 Oct 2018 23:56:19: 68000000 INFO @ Tue, 09 Oct 2018 23:56:21: 68000000 INFO @ Tue, 09 Oct 2018 23:56:21: 81000000 INFO @ Tue, 09 Oct 2018 23:56:25: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:56:25: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:56:25: #1 total tags in treatment: 39627285 INFO @ Tue, 09 Oct 2018 23:56:25: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:56:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:56:26: #1 tags after filtering in treatment: 14617765 INFO @ Tue, 09 Oct 2018 23:56:26: #1 Redundant rate of treatment: 0.63 INFO @ Tue, 09 Oct 2018 23:56:26: #1 finished! INFO @ Tue, 09 Oct 2018 23:56:26: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:56:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:56:27: 69000000 INFO @ Tue, 09 Oct 2018 23:56:27: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:56:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:56:27: Process for pairing-model is terminated! cat: SRX4065333.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4065333.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:56:28: 69000000 INFO @ Tue, 09 Oct 2018 23:56:34: 70000000 INFO @ Tue, 09 Oct 2018 23:56:36: 70000000 INFO @ Tue, 09 Oct 2018 23:56:42: 71000000 INFO @ Tue, 09 Oct 2018 23:56:44: 71000000 INFO @ Tue, 09 Oct 2018 23:56:50: 72000000 INFO @ Tue, 09 Oct 2018 23:56:51: 72000000 INFO @ Tue, 09 Oct 2018 23:56:57: 73000000 INFO @ Tue, 09 Oct 2018 23:56:59: 73000000 INFO @ Tue, 09 Oct 2018 23:57:05: 74000000 INFO @ Tue, 09 Oct 2018 23:57:07: 74000000 INFO @ Tue, 09 Oct 2018 23:57:13: 75000000 INFO @ Tue, 09 Oct 2018 23:57:14: 75000000 INFO @ Tue, 09 Oct 2018 23:57:20: 76000000 INFO @ Tue, 09 Oct 2018 23:57:22: 76000000 INFO @ Tue, 09 Oct 2018 23:57:28: 77000000 INFO @ Tue, 09 Oct 2018 23:57:30: 77000000 INFO @ Tue, 09 Oct 2018 23:57:36: 78000000 INFO @ Tue, 09 Oct 2018 23:57:38: 78000000 INFO @ Tue, 09 Oct 2018 23:57:44: 79000000 INFO @ Tue, 09 Oct 2018 23:57:46: 79000000 INFO @ Tue, 09 Oct 2018 23:57:53: 80000000 INFO @ Tue, 09 Oct 2018 23:57:54: 80000000 INFO @ Tue, 09 Oct 2018 23:58:01: 81000000 INFO @ Tue, 09 Oct 2018 23:58:03: 81000000 INFO @ Tue, 09 Oct 2018 23:58:06: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:58:06: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:58:06: #1 total tags in treatment: 39627285 INFO @ Tue, 09 Oct 2018 23:58:06: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:58:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:58:07: #1 tags after filtering in treatment: 14617765 INFO @ Tue, 09 Oct 2018 23:58:07: #1 Redundant rate of treatment: 0.63 INFO @ Tue, 09 Oct 2018 23:58:07: #1 finished! INFO @ Tue, 09 Oct 2018 23:58:07: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:58:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:58:07: #1 tag size is determined as 50 bps INFO @ Tue, 09 Oct 2018 23:58:07: #1 tag size = 50 INFO @ Tue, 09 Oct 2018 23:58:07: #1 total tags in treatment: 39627285 INFO @ Tue, 09 Oct 2018 23:58:07: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:58:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:58:07: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:58:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:58:07: Process for pairing-model is terminated! cat: SRX4065333.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4065333.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:58:08: #1 tags after filtering in treatment: 14617765 INFO @ Tue, 09 Oct 2018 23:58:08: #1 Redundant rate of treatment: 0.63 INFO @ Tue, 09 Oct 2018 23:58:08: #1 finished! INFO @ Tue, 09 Oct 2018 23:58:08: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:58:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:58:09: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:58:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:58:09: Process for pairing-model is terminated! cat: SRX4065333.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4065333.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4065333.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling