Job ID = 2640886 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-08-24T10:42:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T10:42:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 18,322,059 reads read : 36,644,118 reads written : 18,322,059 reads 0-length : 18,322,059 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:55 18322059 reads; of these: 18322059 (100.00%) were unpaired; of these: 2575796 (14.06%) aligned 0 times 13647178 (74.48%) aligned exactly 1 time 2099085 (11.46%) aligned >1 times 85.94% overall alignment rate Time searching: 00:02:55 Overall time: 00:02:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6284017 / 15746263 = 0.3991 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:52:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:52:21: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:52:21: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:52:27: 1000000 INFO @ Sat, 24 Aug 2019 19:52:34: 2000000 INFO @ Sat, 24 Aug 2019 19:52:41: 3000000 INFO @ Sat, 24 Aug 2019 19:52:47: 4000000 INFO @ Sat, 24 Aug 2019 19:52:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:52:50: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:52:50: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:52:54: 5000000 INFO @ Sat, 24 Aug 2019 19:53:00: 1000000 INFO @ Sat, 24 Aug 2019 19:53:01: 6000000 INFO @ Sat, 24 Aug 2019 19:53:08: 7000000 INFO @ Sat, 24 Aug 2019 19:53:09: 2000000 INFO @ Sat, 24 Aug 2019 19:53:15: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:53:18: 3000000 INFO @ Sat, 24 Aug 2019 19:53:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:53:20: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:53:20: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:53:22: 9000000 INFO @ Sat, 24 Aug 2019 19:53:25: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:53:25: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:53:25: #1 total tags in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:53:25: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:53:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:53:25: #1 tags after filtering in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:53:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:53:25: #1 finished! INFO @ Sat, 24 Aug 2019 19:53:25: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:53:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:53:26: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:53:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:53:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:53:28: 4000000 INFO @ Sat, 24 Aug 2019 19:53:28: 1000000 INFO @ Sat, 24 Aug 2019 19:53:36: 2000000 INFO @ Sat, 24 Aug 2019 19:53:37: 5000000 INFO @ Sat, 24 Aug 2019 19:53:44: 3000000 INFO @ Sat, 24 Aug 2019 19:53:46: 6000000 INFO @ Sat, 24 Aug 2019 19:53:52: 4000000 INFO @ Sat, 24 Aug 2019 19:53:56: 7000000 INFO @ Sat, 24 Aug 2019 19:54:00: 5000000 INFO @ Sat, 24 Aug 2019 19:54:05: 8000000 INFO @ Sat, 24 Aug 2019 19:54:08: 6000000 INFO @ Sat, 24 Aug 2019 19:54:14: 9000000 INFO @ Sat, 24 Aug 2019 19:54:15: 7000000 INFO @ Sat, 24 Aug 2019 19:54:18: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:54:18: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:54:18: #1 total tags in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:54:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:54:19: #1 tags after filtering in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:54:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:54:19: #1 finished! INFO @ Sat, 24 Aug 2019 19:54:19: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:54:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:54:19: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:54:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:54:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:54:23: 8000000 INFO @ Sat, 24 Aug 2019 19:54:30: 9000000 INFO @ Sat, 24 Aug 2019 19:54:34: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:54:34: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:54:34: #1 total tags in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:54:34: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:54:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:54:34: #1 tags after filtering in treatment: 9462246 INFO @ Sat, 24 Aug 2019 19:54:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:54:34: #1 finished! INFO @ Sat, 24 Aug 2019 19:54:34: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:54:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:54:35: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:54:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:54:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944376/SRX3944376.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。