Job ID = 2640878 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,304,301 reads read : 44,608,602 reads written : 22,304,301 reads 0-length : 22,304,301 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:51 22304301 reads; of these: 22304301 (100.00%) were unpaired; of these: 3510882 (15.74%) aligned 0 times 17295131 (77.54%) aligned exactly 1 time 1498288 (6.72%) aligned >1 times 84.26% overall alignment rate Time searching: 00:03:51 Overall time: 00:03:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7887519 / 18793419 = 0.4197 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:50:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:50:15: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:50:15: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:50:23: 1000000 INFO @ Sat, 24 Aug 2019 19:50:30: 2000000 INFO @ Sat, 24 Aug 2019 19:50:37: 3000000 INFO @ Sat, 24 Aug 2019 19:50:44: 4000000 INFO @ Sat, 24 Aug 2019 19:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:50:45: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:50:45: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:50:51: 5000000 INFO @ Sat, 24 Aug 2019 19:50:53: 1000000 INFO @ Sat, 24 Aug 2019 19:50:58: 6000000 INFO @ Sat, 24 Aug 2019 19:51:00: 2000000 INFO @ Sat, 24 Aug 2019 19:51:05: 7000000 INFO @ Sat, 24 Aug 2019 19:51:08: 3000000 INFO @ Sat, 24 Aug 2019 19:51:12: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:51:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:51:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:51:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:51:16: 4000000 INFO @ Sat, 24 Aug 2019 19:51:19: 9000000 INFO @ Sat, 24 Aug 2019 19:51:21: 1000000 INFO @ Sat, 24 Aug 2019 19:51:24: 5000000 INFO @ Sat, 24 Aug 2019 19:51:25: 10000000 INFO @ Sat, 24 Aug 2019 19:51:27: 2000000 INFO @ Sat, 24 Aug 2019 19:51:32: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:51:32: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:51:32: #1 total tags in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:51:32: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:51:32: 6000000 INFO @ Sat, 24 Aug 2019 19:51:32: #1 tags after filtering in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:51:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:51:32: #1 finished! INFO @ Sat, 24 Aug 2019 19:51:32: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:51:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:51:33: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:51:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:51:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:51:34: 3000000 INFO @ Sat, 24 Aug 2019 19:51:40: 7000000 INFO @ Sat, 24 Aug 2019 19:51:40: 4000000 INFO @ Sat, 24 Aug 2019 19:51:46: 5000000 INFO @ Sat, 24 Aug 2019 19:51:47: 8000000 INFO @ Sat, 24 Aug 2019 19:51:53: 6000000 INFO @ Sat, 24 Aug 2019 19:51:55: 9000000 INFO @ Sat, 24 Aug 2019 19:51:59: 7000000 INFO @ Sat, 24 Aug 2019 19:52:03: 10000000 INFO @ Sat, 24 Aug 2019 19:52:05: 8000000 INFO @ Sat, 24 Aug 2019 19:52:09: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:52:09: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:52:09: #1 total tags in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:52:09: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:52:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:52:10: #1 tags after filtering in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:52:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:52:10: #1 finished! INFO @ Sat, 24 Aug 2019 19:52:10: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:52:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:52:11: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:52:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:52:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:52:11: 9000000 INFO @ Sat, 24 Aug 2019 19:52:17: 10000000 INFO @ Sat, 24 Aug 2019 19:52:23: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:52:23: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:52:23: #1 total tags in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:52:23: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:52:23: #1 tags after filtering in treatment: 10905900 INFO @ Sat, 24 Aug 2019 19:52:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:52:23: #1 finished! INFO @ Sat, 24 Aug 2019 19:52:23: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:52:24: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:52:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:52:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944369/SRX3944369.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。