Job ID = 2640876 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,129,024 reads read : 42,258,048 reads written : 21,129,024 reads 0-length : 21,129,024 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:41 21129024 reads; of these: 21129024 (100.00%) were unpaired; of these: 2512946 (11.89%) aligned 0 times 17004586 (80.48%) aligned exactly 1 time 1611492 (7.63%) aligned >1 times 88.11% overall alignment rate Time searching: 00:03:41 Overall time: 00:03:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7381269 / 18616078 = 0.3965 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:51:10: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:51:10: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:51:17: 1000000 INFO @ Sat, 24 Aug 2019 19:51:23: 2000000 INFO @ Sat, 24 Aug 2019 19:51:29: 3000000 INFO @ Sat, 24 Aug 2019 19:51:35: 4000000 INFO @ Sat, 24 Aug 2019 19:51:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:51:40: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:51:40: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:51:42: 5000000 INFO @ Sat, 24 Aug 2019 19:51:46: 1000000 INFO @ Sat, 24 Aug 2019 19:51:50: 6000000 INFO @ Sat, 24 Aug 2019 19:51:52: 2000000 INFO @ Sat, 24 Aug 2019 19:51:57: 7000000 INFO @ Sat, 24 Aug 2019 19:51:59: 3000000 INFO @ Sat, 24 Aug 2019 19:52:04: 8000000 INFO @ Sat, 24 Aug 2019 19:52:05: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:52:10: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:52:10: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:52:11: 9000000 INFO @ Sat, 24 Aug 2019 19:52:12: 5000000 INFO @ Sat, 24 Aug 2019 19:52:18: 10000000 INFO @ Sat, 24 Aug 2019 19:52:18: 1000000 INFO @ Sat, 24 Aug 2019 19:52:20: 6000000 INFO @ Sat, 24 Aug 2019 19:52:25: 2000000 INFO @ Sat, 24 Aug 2019 19:52:25: 11000000 INFO @ Sat, 24 Aug 2019 19:52:27: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:52:27: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:52:27: #1 total tags in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:52:27: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:52:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:52:27: 7000000 INFO @ Sat, 24 Aug 2019 19:52:27: #1 tags after filtering in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:52:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:52:27: #1 finished! INFO @ Sat, 24 Aug 2019 19:52:27: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:52:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:52:28: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:52:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:52:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:52:33: 3000000 INFO @ Sat, 24 Aug 2019 19:52:34: 8000000 INFO @ Sat, 24 Aug 2019 19:52:40: 4000000 INFO @ Sat, 24 Aug 2019 19:52:41: 9000000 INFO @ Sat, 24 Aug 2019 19:52:47: 5000000 INFO @ Sat, 24 Aug 2019 19:52:48: 10000000 INFO @ Sat, 24 Aug 2019 19:52:54: 6000000 INFO @ Sat, 24 Aug 2019 19:52:55: 11000000 INFO @ Sat, 24 Aug 2019 19:52:57: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:52:57: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:52:57: #1 total tags in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:52:57: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:52:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:52:57: #1 tags after filtering in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:52:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:52:57: #1 finished! INFO @ Sat, 24 Aug 2019 19:52:57: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:52:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:52:58: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:52:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:52:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:53:00: 7000000 INFO @ Sat, 24 Aug 2019 19:53:06: 8000000 INFO @ Sat, 24 Aug 2019 19:53:12: 9000000 INFO @ Sat, 24 Aug 2019 19:53:18: 10000000 INFO @ Sat, 24 Aug 2019 19:53:24: 11000000 INFO @ Sat, 24 Aug 2019 19:53:26: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:53:26: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:53:26: #1 total tags in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:53:26: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:53:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:53:26: #1 tags after filtering in treatment: 11234809 INFO @ Sat, 24 Aug 2019 19:53:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:53:26: #1 finished! INFO @ Sat, 24 Aug 2019 19:53:26: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:53:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:53:27: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:53:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:53:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944367/SRX3944367.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。