Job ID = 2640874 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,508,522 reads read : 39,017,044 reads written : 19,508,522 reads 0-length : 19,508,522 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:05 19508522 reads; of these: 19508522 (100.00%) were unpaired; of these: 2165518 (11.10%) aligned 0 times 15879060 (81.40%) aligned exactly 1 time 1463944 (7.50%) aligned >1 times 88.90% overall alignment rate Time searching: 00:03:05 Overall time: 00:03:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6544911 / 17343004 = 0.3774 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:47:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:47:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:47:58: 1000000 INFO @ Sat, 24 Aug 2019 19:48:05: 2000000 INFO @ Sat, 24 Aug 2019 19:48:12: 3000000 INFO @ Sat, 24 Aug 2019 19:48:19: 4000000 INFO @ Sat, 24 Aug 2019 19:48:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:48:21: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:48:21: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:48:27: 5000000 INFO @ Sat, 24 Aug 2019 19:48:28: 1000000 INFO @ Sat, 24 Aug 2019 19:48:34: 6000000 INFO @ Sat, 24 Aug 2019 19:48:35: 2000000 INFO @ Sat, 24 Aug 2019 19:48:41: 7000000 INFO @ Sat, 24 Aug 2019 19:48:42: 3000000 INFO @ Sat, 24 Aug 2019 19:48:48: 8000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:48:50: 4000000 INFO @ Sat, 24 Aug 2019 19:48:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:48:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:48:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:48:56: 9000000 INFO @ Sat, 24 Aug 2019 19:48:57: 5000000 INFO @ Sat, 24 Aug 2019 19:48:58: 1000000 INFO @ Sat, 24 Aug 2019 19:49:03: 10000000 INFO @ Sat, 24 Aug 2019 19:49:04: 6000000 INFO @ Sat, 24 Aug 2019 19:49:06: 2000000 INFO @ Sat, 24 Aug 2019 19:49:09: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:49:09: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:49:09: #1 total tags in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:49:09: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:49:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:49:09: #1 tags after filtering in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:49:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:49:09: #1 finished! INFO @ Sat, 24 Aug 2019 19:49:09: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:49:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:49:10: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:49:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:49:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:49:11: 7000000 INFO @ Sat, 24 Aug 2019 19:49:12: 3000000 INFO @ Sat, 24 Aug 2019 19:49:18: 8000000 INFO @ Sat, 24 Aug 2019 19:49:20: 4000000 INFO @ Sat, 24 Aug 2019 19:49:26: 9000000 INFO @ Sat, 24 Aug 2019 19:49:27: 5000000 INFO @ Sat, 24 Aug 2019 19:49:33: 10000000 INFO @ Sat, 24 Aug 2019 19:49:34: 6000000 INFO @ Sat, 24 Aug 2019 19:49:38: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:49:38: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:49:38: #1 total tags in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:49:38: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:49:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:49:39: #1 tags after filtering in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:49:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:49:39: #1 finished! INFO @ Sat, 24 Aug 2019 19:49:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:49:39: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:49:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:49:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:49:41: 7000000 INFO @ Sat, 24 Aug 2019 19:49:48: 8000000 INFO @ Sat, 24 Aug 2019 19:49:55: 9000000 INFO @ Sat, 24 Aug 2019 19:50:02: 10000000 INFO @ Sat, 24 Aug 2019 19:50:08: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:50:08: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:50:08: #1 total tags in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:50:08: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:50:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:50:08: #1 tags after filtering in treatment: 10798093 INFO @ Sat, 24 Aug 2019 19:50:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:50:08: #1 finished! INFO @ Sat, 24 Aug 2019 19:50:08: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:50:09: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:50:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:50:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944366/SRX3944366.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。