Job ID = 2640869 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,150,101 reads read : 36,300,202 reads written : 18,150,101 reads 0-length : 18,150,101 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 18150101 reads; of these: 18150101 (100.00%) were unpaired; of these: 3194696 (17.60%) aligned 0 times 13386620 (73.76%) aligned exactly 1 time 1568785 (8.64%) aligned >1 times 82.40% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5599546 / 14955405 = 0.3744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:46:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:46:18: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:46:18: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:46:27: 1000000 INFO @ Sat, 24 Aug 2019 19:46:36: 2000000 INFO @ Sat, 24 Aug 2019 19:46:44: 3000000 INFO @ Sat, 24 Aug 2019 19:46:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:46:48: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:46:48: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:46:53: 4000000 INFO @ Sat, 24 Aug 2019 19:46:58: 1000000 INFO @ Sat, 24 Aug 2019 19:47:02: 5000000 INFO @ Sat, 24 Aug 2019 19:47:09: 2000000 INFO @ Sat, 24 Aug 2019 19:47:11: 6000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:47:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:47:18: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:47:18: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:47:19: 3000000 INFO @ Sat, 24 Aug 2019 19:47:19: 7000000 INFO @ Sat, 24 Aug 2019 19:47:27: 1000000 INFO @ Sat, 24 Aug 2019 19:47:28: 8000000 INFO @ Sat, 24 Aug 2019 19:47:29: 4000000 INFO @ Sat, 24 Aug 2019 19:47:36: 2000000 INFO @ Sat, 24 Aug 2019 19:47:38: 9000000 INFO @ Sat, 24 Aug 2019 19:47:39: 5000000 INFO @ Sat, 24 Aug 2019 19:47:40: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:47:40: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:47:40: #1 total tags in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:47:40: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:47:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:47:41: #1 tags after filtering in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:47:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:47:41: #1 finished! INFO @ Sat, 24 Aug 2019 19:47:41: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:47:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:47:41: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:47:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:47:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:47:45: 3000000 INFO @ Sat, 24 Aug 2019 19:47:50: 6000000 INFO @ Sat, 24 Aug 2019 19:47:54: 4000000 INFO @ Sat, 24 Aug 2019 19:48:00: 7000000 INFO @ Sat, 24 Aug 2019 19:48:03: 5000000 INFO @ Sat, 24 Aug 2019 19:48:10: 8000000 INFO @ Sat, 24 Aug 2019 19:48:12: 6000000 INFO @ Sat, 24 Aug 2019 19:48:20: 9000000 INFO @ Sat, 24 Aug 2019 19:48:20: 7000000 INFO @ Sat, 24 Aug 2019 19:48:23: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:48:23: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:48:23: #1 total tags in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:48:23: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:48:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:48:24: #1 tags after filtering in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:48:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:48:24: #1 finished! INFO @ Sat, 24 Aug 2019 19:48:24: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:48:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:48:24: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:48:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:48:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:48:29: 8000000 INFO @ Sat, 24 Aug 2019 19:48:37: 9000000 INFO @ Sat, 24 Aug 2019 19:48:40: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:48:40: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:48:40: #1 total tags in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:48:40: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:48:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:48:40: #1 tags after filtering in treatment: 9355859 INFO @ Sat, 24 Aug 2019 19:48:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:48:40: #1 finished! INFO @ Sat, 24 Aug 2019 19:48:40: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:48:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:48:41: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:48:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:48:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944364/SRX3944364.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。