Job ID = 2640867 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,004,234 reads read : 40,008,468 reads written : 20,004,234 reads 0-length : 20,004,234 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 20004234 reads; of these: 20004234 (100.00%) were unpaired; of these: 3554177 (17.77%) aligned 0 times 14809796 (74.03%) aligned exactly 1 time 1640261 (8.20%) aligned >1 times 82.23% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7054178 / 16450057 = 0.4288 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:46:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:46:44: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:46:44: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:46:53: 1000000 INFO @ Sat, 24 Aug 2019 19:47:01: 2000000 INFO @ Sat, 24 Aug 2019 19:47:09: 3000000 INFO @ Sat, 24 Aug 2019 19:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:47:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:47:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:47:18: 4000000 INFO @ Sat, 24 Aug 2019 19:47:22: 1000000 INFO @ Sat, 24 Aug 2019 19:47:26: 5000000 INFO @ Sat, 24 Aug 2019 19:47:31: 2000000 INFO @ Sat, 24 Aug 2019 19:47:35: 6000000 INFO @ Sat, 24 Aug 2019 19:47:40: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:47:43: 7000000 INFO @ Sat, 24 Aug 2019 19:47:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:47:44: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:47:44: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:47:49: 4000000 INFO @ Sat, 24 Aug 2019 19:47:53: 1000000 INFO @ Sat, 24 Aug 2019 19:47:53: 8000000 INFO @ Sat, 24 Aug 2019 19:47:59: 5000000 INFO @ Sat, 24 Aug 2019 19:48:01: 2000000 INFO @ Sat, 24 Aug 2019 19:48:03: 9000000 INFO @ Sat, 24 Aug 2019 19:48:06: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:48:06: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:48:06: #1 total tags in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:48:06: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:48:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:48:07: #1 tags after filtering in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:48:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:48:07: #1 finished! INFO @ Sat, 24 Aug 2019 19:48:07: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:48:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:48:07: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:48:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:48:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:48:07: 6000000 INFO @ Sat, 24 Aug 2019 19:48:10: 3000000 INFO @ Sat, 24 Aug 2019 19:48:16: 7000000 INFO @ Sat, 24 Aug 2019 19:48:18: 4000000 INFO @ Sat, 24 Aug 2019 19:48:25: 8000000 INFO @ Sat, 24 Aug 2019 19:48:27: 5000000 INFO @ Sat, 24 Aug 2019 19:48:34: 9000000 INFO @ Sat, 24 Aug 2019 19:48:35: 6000000 INFO @ Sat, 24 Aug 2019 19:48:37: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:48:37: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:48:37: #1 total tags in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:48:37: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:48:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:48:37: #1 tags after filtering in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:48:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:48:37: #1 finished! INFO @ Sat, 24 Aug 2019 19:48:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:48:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:48:38: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:48:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:48:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:48:43: 7000000 INFO @ Sat, 24 Aug 2019 19:48:52: 8000000 INFO @ Sat, 24 Aug 2019 19:49:00: 9000000 INFO @ Sat, 24 Aug 2019 19:49:03: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:49:03: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:49:03: #1 total tags in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:49:03: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:49:03: #1 tags after filtering in treatment: 9395879 INFO @ Sat, 24 Aug 2019 19:49:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:49:03: #1 finished! INFO @ Sat, 24 Aug 2019 19:49:03: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:49:04: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:49:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:49:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944362/SRX3944362.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。