Job ID = 2640854 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,590,551 reads read : 49,181,102 reads written : 24,590,551 reads 0-length : 24,590,551 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:03 24590551 reads; of these: 24590551 (100.00%) were unpaired; of these: 3622921 (14.73%) aligned 0 times 19811219 (80.56%) aligned exactly 1 time 1156411 (4.70%) aligned >1 times 85.27% overall alignment rate Time searching: 00:04:03 Overall time: 00:04:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10208714 / 20967630 = 0.4869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:44:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:44:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:44:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:44:15: 1000000 INFO @ Sat, 24 Aug 2019 19:44:22: 2000000 INFO @ Sat, 24 Aug 2019 19:44:29: 3000000 INFO @ Sat, 24 Aug 2019 19:44:35: 4000000 INFO @ Sat, 24 Aug 2019 19:44:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:44:38: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:44:38: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:44:42: 5000000 INFO @ Sat, 24 Aug 2019 19:44:45: 1000000 INFO @ Sat, 24 Aug 2019 19:44:48: 6000000 INFO @ Sat, 24 Aug 2019 19:44:51: 2000000 INFO @ Sat, 24 Aug 2019 19:44:54: 7000000 INFO @ Sat, 24 Aug 2019 19:44:56: 3000000 INFO @ Sat, 24 Aug 2019 19:45:01: 8000000 INFO @ Sat, 24 Aug 2019 19:45:02: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:45:07: 9000000 INFO @ Sat, 24 Aug 2019 19:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:45:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:45:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:45:08: 5000000 INFO @ Sat, 24 Aug 2019 19:45:14: 10000000 INFO @ Sat, 24 Aug 2019 19:45:14: 6000000 INFO @ Sat, 24 Aug 2019 19:45:15: 1000000 INFO @ Sat, 24 Aug 2019 19:45:18: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:45:18: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:45:18: #1 total tags in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:45:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:45:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:45:19: #1 tags after filtering in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:45:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:45:19: #1 finished! INFO @ Sat, 24 Aug 2019 19:45:19: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:45:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:45:19: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:45:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:45:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:45:20: 7000000 INFO @ Sat, 24 Aug 2019 19:45:22: 2000000 INFO @ Sat, 24 Aug 2019 19:45:26: 8000000 INFO @ Sat, 24 Aug 2019 19:45:29: 3000000 INFO @ Sat, 24 Aug 2019 19:45:32: 9000000 INFO @ Sat, 24 Aug 2019 19:45:36: 4000000 INFO @ Sat, 24 Aug 2019 19:45:38: 10000000 INFO @ Sat, 24 Aug 2019 19:45:42: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:45:42: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:45:42: #1 total tags in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:45:42: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:45:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:45:42: #1 tags after filtering in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:45:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:45:42: #1 finished! INFO @ Sat, 24 Aug 2019 19:45:42: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:45:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:45:43: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:45:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:45:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:45:43: 5000000 INFO @ Sat, 24 Aug 2019 19:45:51: 6000000 INFO @ Sat, 24 Aug 2019 19:45:58: 7000000 INFO @ Sat, 24 Aug 2019 19:46:05: 8000000 INFO @ Sat, 24 Aug 2019 19:46:12: 9000000 INFO @ Sat, 24 Aug 2019 19:46:19: 10000000 INFO @ Sat, 24 Aug 2019 19:46:24: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:46:24: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:46:24: #1 total tags in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:46:24: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:46:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:46:24: #1 tags after filtering in treatment: 10758916 INFO @ Sat, 24 Aug 2019 19:46:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:46:24: #1 finished! INFO @ Sat, 24 Aug 2019 19:46:24: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:46:25: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:46:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:46:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944351/SRX3944351.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。