Job ID = 2640852 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,309,789 reads read : 44,619,578 reads written : 22,309,789 reads 0-length : 22,309,789 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:53 22309789 reads; of these: 22309789 (100.00%) were unpaired; of these: 4141499 (18.56%) aligned 0 times 16905714 (75.78%) aligned exactly 1 time 1262576 (5.66%) aligned >1 times 81.44% overall alignment rate Time searching: 00:03:53 Overall time: 00:03:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7602378 / 18168290 = 0.4184 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:42:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:42:28: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:42:28: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:42:35: 1000000 INFO @ Sat, 24 Aug 2019 19:42:41: 2000000 INFO @ Sat, 24 Aug 2019 19:42:48: 3000000 INFO @ Sat, 24 Aug 2019 19:42:55: 4000000 INFO @ Sat, 24 Aug 2019 19:42:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:42:57: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:42:57: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:43:02: 5000000 INFO @ Sat, 24 Aug 2019 19:43:06: 1000000 INFO @ Sat, 24 Aug 2019 19:43:09: 6000000 INFO @ Sat, 24 Aug 2019 19:43:15: 2000000 INFO @ Sat, 24 Aug 2019 19:43:16: 7000000 INFO @ Sat, 24 Aug 2019 19:43:22: 8000000 INFO @ Sat, 24 Aug 2019 19:43:24: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:43:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:43:27: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:43:27: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:43:29: 9000000 INFO @ Sat, 24 Aug 2019 19:43:33: 4000000 INFO @ Sat, 24 Aug 2019 19:43:35: 1000000 INFO @ Sat, 24 Aug 2019 19:43:37: 10000000 INFO @ Sat, 24 Aug 2019 19:43:40: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:43:40: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:43:40: #1 total tags in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:43:40: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:43:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:43:41: #1 tags after filtering in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:43:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:43:41: #1 finished! INFO @ Sat, 24 Aug 2019 19:43:41: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:43:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:43:41: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:43:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:43:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:43:44: 2000000 INFO @ Sat, 24 Aug 2019 19:43:44: 5000000 INFO @ Sat, 24 Aug 2019 19:43:51: 3000000 INFO @ Sat, 24 Aug 2019 19:43:53: 6000000 INFO @ Sat, 24 Aug 2019 19:43:59: 4000000 INFO @ Sat, 24 Aug 2019 19:44:02: 7000000 INFO @ Sat, 24 Aug 2019 19:44:06: 5000000 INFO @ Sat, 24 Aug 2019 19:44:11: 8000000 INFO @ Sat, 24 Aug 2019 19:44:13: 6000000 INFO @ Sat, 24 Aug 2019 19:44:19: 9000000 INFO @ Sat, 24 Aug 2019 19:44:21: 7000000 INFO @ Sat, 24 Aug 2019 19:44:28: 10000000 INFO @ Sat, 24 Aug 2019 19:44:28: 8000000 INFO @ Sat, 24 Aug 2019 19:44:32: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:44:32: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:44:32: #1 total tags in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:44:32: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:44:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:44:33: #1 tags after filtering in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:44:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:44:33: #1 finished! INFO @ Sat, 24 Aug 2019 19:44:33: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:44:33: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:44:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:44:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:44:36: 9000000 INFO @ Sat, 24 Aug 2019 19:44:44: 10000000 INFO @ Sat, 24 Aug 2019 19:44:48: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:44:48: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:44:48: #1 total tags in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:44:48: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:44:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:44:48: #1 tags after filtering in treatment: 10565912 INFO @ Sat, 24 Aug 2019 19:44:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:44:48: #1 finished! INFO @ Sat, 24 Aug 2019 19:44:48: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:44:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:44:49: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:44:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:44:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944349/SRX3944349.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。