Job ID = 2640848 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,582,351 reads read : 45,164,702 reads written : 22,582,351 reads 0-length : 22,582,351 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:51 22582351 reads; of these: 22582351 (100.00%) were unpaired; of these: 2381342 (10.55%) aligned 0 times 19044512 (84.33%) aligned exactly 1 time 1156497 (5.12%) aligned >1 times 89.45% overall alignment rate Time searching: 00:03:51 Overall time: 00:03:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10320306 / 20201009 = 0.5109 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:41:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:41:43: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:41:43: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:41:51: 1000000 INFO @ Sat, 24 Aug 2019 19:41:58: 2000000 INFO @ Sat, 24 Aug 2019 19:42:06: 3000000 INFO @ Sat, 24 Aug 2019 19:42:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:42:12: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:42:12: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:42:13: 4000000 INFO @ Sat, 24 Aug 2019 19:42:19: 1000000 INFO @ Sat, 24 Aug 2019 19:42:21: 5000000 INFO @ Sat, 24 Aug 2019 19:42:25: 2000000 INFO @ Sat, 24 Aug 2019 19:42:28: 6000000 INFO @ Sat, 24 Aug 2019 19:42:31: 3000000 INFO @ Sat, 24 Aug 2019 19:42:36: 7000000 INFO @ Sat, 24 Aug 2019 19:42:37: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:42:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:42:42: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:42:42: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:42:43: 5000000 INFO @ Sat, 24 Aug 2019 19:42:43: 8000000 INFO @ Sat, 24 Aug 2019 19:42:49: 6000000 INFO @ Sat, 24 Aug 2019 19:42:50: 1000000 INFO @ Sat, 24 Aug 2019 19:42:51: 9000000 INFO @ Sat, 24 Aug 2019 19:42:55: 7000000 INFO @ Sat, 24 Aug 2019 19:42:57: 2000000 INFO @ Sat, 24 Aug 2019 19:42:58: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:42:58: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:42:58: #1 total tags in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:42:58: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:42:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:42:58: #1 tags after filtering in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:42:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:42:58: #1 finished! INFO @ Sat, 24 Aug 2019 19:42:58: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:42:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:42:59: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:42:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:42:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:43:01: 8000000 INFO @ Sat, 24 Aug 2019 19:43:05: 3000000 INFO @ Sat, 24 Aug 2019 19:43:07: 9000000 INFO @ Sat, 24 Aug 2019 19:43:12: 4000000 INFO @ Sat, 24 Aug 2019 19:43:13: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:43:13: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:43:13: #1 total tags in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:43:13: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:43:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:43:13: #1 tags after filtering in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:43:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:43:13: #1 finished! INFO @ Sat, 24 Aug 2019 19:43:13: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:43:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:43:14: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:43:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:43:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:43:20: 5000000 INFO @ Sat, 24 Aug 2019 19:43:27: 6000000 INFO @ Sat, 24 Aug 2019 19:43:34: 7000000 INFO @ Sat, 24 Aug 2019 19:43:41: 8000000 INFO @ Sat, 24 Aug 2019 19:43:49: 9000000 INFO @ Sat, 24 Aug 2019 19:43:55: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:43:55: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:43:55: #1 total tags in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:43:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:43:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:43:55: #1 tags after filtering in treatment: 9880703 INFO @ Sat, 24 Aug 2019 19:43:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:43:55: #1 finished! INFO @ Sat, 24 Aug 2019 19:43:55: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:43:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:43:56: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:43:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:43:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3944346/SRX3944346.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。