Job ID = 2010766 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,675,894 reads read : 4,675,894 reads written : 4,675,894 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:47 4675894 reads; of these: 4675894 (100.00%) were unpaired; of these: 462274 (9.89%) aligned 0 times 3959951 (84.69%) aligned exactly 1 time 253669 (5.43%) aligned >1 times 90.11% overall alignment rate Time searching: 00:00:47 Overall time: 00:00:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 783850 / 4213620 = 0.1860 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:31:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:25: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:25: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:25: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:25: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:26: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:26: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:31: 1000000 INFO @ Sat, 06 Jul 2019 00:31:33: 1000000 INFO @ Sat, 06 Jul 2019 00:31:33: 1000000 INFO @ Sat, 06 Jul 2019 00:31:38: 2000000 INFO @ Sat, 06 Jul 2019 00:31:39: 2000000 INFO @ Sat, 06 Jul 2019 00:31:41: 2000000 INFO @ Sat, 06 Jul 2019 00:31:44: 3000000 INFO @ Sat, 06 Jul 2019 00:31:46: 3000000 INFO @ Sat, 06 Jul 2019 00:31:47: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:31:47: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:31:47: #1 total tags in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:47: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:31:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:31:47: #1 tags after filtering in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:31:47: #1 finished! INFO @ Sat, 06 Jul 2019 00:31:47: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:31:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:31:47: #2 number of paired peaks: 18 WARNING @ Sat, 06 Jul 2019 00:31:47: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:31:47: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:31:48: 3000000 INFO @ Sat, 06 Jul 2019 00:31:48: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:31:48: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:31:48: #1 total tags in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:48: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:31:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:31:49: #1 tags after filtering in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:31:49: #1 finished! INFO @ Sat, 06 Jul 2019 00:31:49: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:31:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:31:49: #2 number of paired peaks: 18 WARNING @ Sat, 06 Jul 2019 00:31:49: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:31:49: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:31:52: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:31:52: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:31:52: #1 total tags in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:52: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:31:52: #1 tags after filtering in treatment: 3429770 INFO @ Sat, 06 Jul 2019 00:31:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 00:31:52: #1 finished! INFO @ Sat, 06 Jul 2019 00:31:52: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:31:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:31:52: #2 number of paired peaks: 18 WARNING @ Sat, 06 Jul 2019 00:31:52: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:31:52: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10_peaks.narrowPeak : No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX390613/SRX390613.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。