Job ID = 2010762 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 50,212,540 reads read : 50,212,540 reads written : 50,212,540 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:05 50212540 reads; of these: 50212540 (100.00%) were unpaired; of these: 24047410 (47.89%) aligned 0 times 23421063 (46.64%) aligned exactly 1 time 2744067 (5.46%) aligned >1 times 52.11% overall alignment rate Time searching: 00:07:05 Overall time: 00:07:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 22128174 / 26165130 = 0.8457 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:02:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:02:42: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:02:42: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:02:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:02:43: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:02:43: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:02:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:02:44: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:02:44: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:02:50: 1000000 INFO @ Sat, 06 Jul 2019 01:02:52: 1000000 INFO @ Sat, 06 Jul 2019 01:02:52: 1000000 INFO @ Sat, 06 Jul 2019 01:02:58: 2000000 INFO @ Sat, 06 Jul 2019 01:02:59: 2000000 INFO @ Sat, 06 Jul 2019 01:03:00: 2000000 INFO @ Sat, 06 Jul 2019 01:03:06: 3000000 INFO @ Sat, 06 Jul 2019 01:03:06: 3000000 INFO @ Sat, 06 Jul 2019 01:03:08: 3000000 INFO @ Sat, 06 Jul 2019 01:03:13: 4000000 INFO @ Sat, 06 Jul 2019 01:03:14: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:03:14: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:03:14: #1 total tags in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:14: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:03:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:03:14: #1 tags after filtering in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:03:14: #1 finished! INFO @ Sat, 06 Jul 2019 01:03:14: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:03:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:03:14: #2 number of paired peaks: 28 WARNING @ Sat, 06 Jul 2019 01:03:14: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:03:14: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:03:14: 4000000 INFO @ Sat, 06 Jul 2019 01:03:15: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:03:15: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:03:15: #1 total tags in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:15: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:03:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:03:15: #1 tags after filtering in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:03:15: #1 finished! INFO @ Sat, 06 Jul 2019 01:03:15: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:03:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:03:15: #2 number of paired peaks: 28 WARNING @ Sat, 06 Jul 2019 01:03:15: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:03:15: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:03:15: 4000000 INFO @ Sat, 06 Jul 2019 01:03:16: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 01:03:16: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 01:03:16: #1 total tags in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:16: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:03:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:03:16: #1 tags after filtering in treatment: 4036956 INFO @ Sat, 06 Jul 2019 01:03:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:03:16: #1 finished! INFO @ Sat, 06 Jul 2019 01:03:16: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:03:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:03:16: #2 number of paired peaks: 28 WARNING @ Sat, 06 Jul 2019 01:03:16: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:03:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20_peaks.narrowPeakcut: /home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05_peaks.narrowPeak: No such file or directory : No such file or directory pass1 - making usageList (0 chroms)pass1 - making usageList (0 chroms)pass1 - making usageList (0 chroms): 2 millis : 2 millis : 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184)needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05_model.r’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.05_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387220/SRX387220.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。