Job ID = 2010756 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 49,724,412 reads read : 49,724,412 reads written : 49,724,412 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:27 49724412 reads; of these: 49724412 (100.00%) were unpaired; of these: 2375113 (4.78%) aligned 0 times 42937962 (86.35%) aligned exactly 1 time 4411337 (8.87%) aligned >1 times 95.22% overall alignment rate Time searching: 00:08:27 Overall time: 00:08:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 31414887 / 47349299 = 0.6635 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:03:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:22: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:22: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:23: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:23: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:03:24: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:03:24: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:03:31: 1000000 INFO @ Sat, 06 Jul 2019 01:03:32: 1000000 INFO @ Sat, 06 Jul 2019 01:03:33: 1000000 INFO @ Sat, 06 Jul 2019 01:03:39: 2000000 INFO @ Sat, 06 Jul 2019 01:03:41: 2000000 INFO @ Sat, 06 Jul 2019 01:03:42: 2000000 INFO @ Sat, 06 Jul 2019 01:03:48: 3000000 INFO @ Sat, 06 Jul 2019 01:03:49: 3000000 INFO @ Sat, 06 Jul 2019 01:03:50: 3000000 INFO @ Sat, 06 Jul 2019 01:03:56: 4000000 INFO @ Sat, 06 Jul 2019 01:03:57: 4000000 INFO @ Sat, 06 Jul 2019 01:03:58: 4000000 INFO @ Sat, 06 Jul 2019 01:04:04: 5000000 INFO @ Sat, 06 Jul 2019 01:04:05: 5000000 INFO @ Sat, 06 Jul 2019 01:04:07: 5000000 INFO @ Sat, 06 Jul 2019 01:04:12: 6000000 INFO @ Sat, 06 Jul 2019 01:04:13: 6000000 INFO @ Sat, 06 Jul 2019 01:04:15: 6000000 INFO @ Sat, 06 Jul 2019 01:04:21: 7000000 INFO @ Sat, 06 Jul 2019 01:04:22: 7000000 INFO @ Sat, 06 Jul 2019 01:04:24: 7000000 INFO @ Sat, 06 Jul 2019 01:04:29: 8000000 INFO @ Sat, 06 Jul 2019 01:04:31: 8000000 INFO @ Sat, 06 Jul 2019 01:04:34: 8000000 INFO @ Sat, 06 Jul 2019 01:04:37: 9000000 INFO @ Sat, 06 Jul 2019 01:04:41: 9000000 INFO @ Sat, 06 Jul 2019 01:04:44: 9000000 INFO @ Sat, 06 Jul 2019 01:04:45: 10000000 INFO @ Sat, 06 Jul 2019 01:04:50: 10000000 INFO @ Sat, 06 Jul 2019 01:04:52: 11000000 INFO @ Sat, 06 Jul 2019 01:04:53: 10000000 INFO @ Sat, 06 Jul 2019 01:05:00: 11000000 INFO @ Sat, 06 Jul 2019 01:05:00: 12000000 INFO @ Sat, 06 Jul 2019 01:05:03: 11000000 INFO @ Sat, 06 Jul 2019 01:05:08: 13000000 INFO @ Sat, 06 Jul 2019 01:05:10: 12000000 INFO @ Sat, 06 Jul 2019 01:05:13: 12000000 INFO @ Sat, 06 Jul 2019 01:05:16: 14000000 INFO @ Sat, 06 Jul 2019 01:05:20: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:05:23: 13000000 INFO @ Sat, 06 Jul 2019 01:05:23: 15000000 INFO @ Sat, 06 Jul 2019 01:05:29: 14000000 INFO @ Sat, 06 Jul 2019 01:05:30: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:05:30: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:05:30: #1 total tags in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:30: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:31: #1 tags after filtering in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:31: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:31: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:32: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:05:33: 14000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 01:05:39: 15000000 INFO @ Sat, 06 Jul 2019 01:05:42: 15000000 INFO @ Sat, 06 Jul 2019 01:05:48: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:05:48: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:05:48: #1 total tags in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:48: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:48: #1 tags after filtering in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:48: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:48: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:49: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:49: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:05:51: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:05:51: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:05:51: #1 total tags in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:51: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:05:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:05:51: #1 tags after filtering in treatment: 15934412 INFO @ Sat, 06 Jul 2019 01:05:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:05:51: #1 finished! INFO @ Sat, 06 Jul 2019 01:05:51: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:05:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:05:52: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:05:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05_model.r’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20_*.xls’: No such file or directory rm: rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05_*.xls’cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.20_peaks.narrowPeak’: No such file or directory: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX387214/SRX387214.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling