Job ID = 2010734 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 7,709,675 reads read : 15,419,350 reads written : 15,419,350 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:59 7709675 reads; of these: 7709675 (100.00%) were paired; of these: 1354636 (17.57%) aligned concordantly 0 times 5620821 (72.91%) aligned concordantly exactly 1 time 734218 (9.52%) aligned concordantly >1 times ---- 1354636 pairs aligned concordantly 0 times; of these: 102303 (7.55%) aligned discordantly 1 time ---- 1252333 pairs aligned 0 times concordantly or discordantly; of these: 2504666 mates make up the pairs; of these: 2267762 (90.54%) aligned 0 times 160395 (6.40%) aligned exactly 1 time 76509 (3.05%) aligned >1 times 85.29% overall alignment rate Time searching: 00:06:59 Overall time: 00:06:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 94624 / 6438231 = 0.0147 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:31:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:45: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:45: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:31:47: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:31:47: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:31:53: 1000000 INFO @ Sat, 06 Jul 2019 00:31:54: 1000000 INFO @ Sat, 06 Jul 2019 00:31:55: 1000000 INFO @ Sat, 06 Jul 2019 00:32:01: 2000000 INFO @ Sat, 06 Jul 2019 00:32:03: 2000000 INFO @ Sat, 06 Jul 2019 00:32:04: 2000000 INFO @ Sat, 06 Jul 2019 00:32:09: 3000000 INFO @ Sat, 06 Jul 2019 00:32:12: 3000000 INFO @ Sat, 06 Jul 2019 00:32:13: 3000000 INFO @ Sat, 06 Jul 2019 00:32:17: 4000000 INFO @ Sat, 06 Jul 2019 00:32:21: 4000000 INFO @ Sat, 06 Jul 2019 00:32:21: 4000000 INFO @ Sat, 06 Jul 2019 00:32:26: 5000000 INFO @ Sat, 06 Jul 2019 00:32:29: 5000000 INFO @ Sat, 06 Jul 2019 00:32:30: 5000000 INFO @ Sat, 06 Jul 2019 00:32:34: 6000000 INFO @ Sat, 06 Jul 2019 00:32:38: 6000000 INFO @ Sat, 06 Jul 2019 00:32:39: 6000000 INFO @ Sat, 06 Jul 2019 00:32:42: 7000000 INFO @ Sat, 06 Jul 2019 00:32:46: 7000000 INFO @ Sat, 06 Jul 2019 00:32:47: 7000000 INFO @ Sat, 06 Jul 2019 00:32:50: 8000000 INFO @ Sat, 06 Jul 2019 00:32:55: 8000000 INFO @ Sat, 06 Jul 2019 00:32:56: 8000000 INFO @ Sat, 06 Jul 2019 00:32:58: 9000000 INFO @ Sat, 06 Jul 2019 00:33:04: 9000000 INFO @ Sat, 06 Jul 2019 00:33:05: 9000000 INFO @ Sat, 06 Jul 2019 00:33:05: 10000000 INFO @ Sat, 06 Jul 2019 00:33:12: 10000000 INFO @ Sat, 06 Jul 2019 00:33:13: 10000000 INFO @ Sat, 06 Jul 2019 00:33:13: 11000000 INFO @ Sat, 06 Jul 2019 00:33:21: 11000000 INFO @ Sat, 06 Jul 2019 00:33:21: 12000000 INFO @ Sat, 06 Jul 2019 00:33:22: 11000000 INFO @ Sat, 06 Jul 2019 00:33:28: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:33:28: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:33:28: #1 total tags in treatment: 6261270 INFO @ Sat, 06 Jul 2019 00:33:28: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:33:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:33:29: #1 tags after filtering in treatment: 5087843 INFO @ Sat, 06 Jul 2019 00:33:29: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:33:29: #1 finished! INFO @ Sat, 06 Jul 2019 00:33:29: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:33:29: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:33:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:33:29: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:33:29: 12000000 INFO @ Sat, 06 Jul 2019 00:33:30: 12000000 INFO @ Sat, 06 Jul 2019 00:33:37: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:33:37: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:33:37: #1 total tags in treatment: 6261270 INFO @ Sat, 06 Jul 2019 00:33:37: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:33:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:33:37: #1 tags after filtering in treatment: 5087843 INFO @ Sat, 06 Jul 2019 00:33:37: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:33:37: #1 finished! INFO @ Sat, 06 Jul 2019 00:33:37: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:33:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:33:38: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:33:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:33:38: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:33:38: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:33:38: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:33:38: #1 total tags in treatment: 6261270 INFO @ Sat, 06 Jul 2019 00:33:38: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:33:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:33:38: #1 tags after filtering in treatment: 5087843 INFO @ Sat, 06 Jul 2019 00:33:38: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:33:38: #1 finished! INFO @ Sat, 06 Jul 2019 00:33:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:33:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:33:38: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:33:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:33:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386915/SRX386915.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。