Job ID = 2010713 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 7,609,720 reads read : 15,219,440 reads written : 15,219,440 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:57 7609720 reads; of these: 7609720 (100.00%) were paired; of these: 1470912 (19.33%) aligned concordantly 0 times 5320116 (69.91%) aligned concordantly exactly 1 time 818692 (10.76%) aligned concordantly >1 times ---- 1470912 pairs aligned concordantly 0 times; of these: 83772 (5.70%) aligned discordantly 1 time ---- 1387140 pairs aligned 0 times concordantly or discordantly; of these: 2774280 mates make up the pairs; of these: 2425024 (87.41%) aligned 0 times 265884 (9.58%) aligned exactly 1 time 83372 (3.01%) aligned >1 times 84.07% overall alignment rate Time searching: 00:06:57 Overall time: 00:06:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 93805 / 6205560 = 0.0151 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:25:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:25:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:25:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:25:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:25:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:25:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:25:32: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:25:32: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:25:38: 1000000 INFO @ Sat, 06 Jul 2019 00:25:40: 1000000 INFO @ Sat, 06 Jul 2019 00:25:42: 1000000 INFO @ Sat, 06 Jul 2019 00:25:46: 2000000 INFO @ Sat, 06 Jul 2019 00:25:49: 2000000 INFO @ Sat, 06 Jul 2019 00:25:53: 2000000 INFO @ Sat, 06 Jul 2019 00:25:54: 3000000 INFO @ Sat, 06 Jul 2019 00:25:59: 3000000 INFO @ Sat, 06 Jul 2019 00:26:02: 4000000 INFO @ Sat, 06 Jul 2019 00:26:03: 3000000 INFO @ Sat, 06 Jul 2019 00:26:08: 4000000 INFO @ Sat, 06 Jul 2019 00:26:10: 5000000 INFO @ Sat, 06 Jul 2019 00:26:14: 4000000 INFO @ Sat, 06 Jul 2019 00:26:17: 5000000 INFO @ Sat, 06 Jul 2019 00:26:18: 6000000 INFO @ Sat, 06 Jul 2019 00:26:24: 5000000 INFO @ Sat, 06 Jul 2019 00:26:26: 6000000 INFO @ Sat, 06 Jul 2019 00:26:26: 7000000 INFO @ Sat, 06 Jul 2019 00:26:34: 8000000 INFO @ Sat, 06 Jul 2019 00:26:34: 6000000 INFO @ Sat, 06 Jul 2019 00:26:35: 7000000 INFO @ Sat, 06 Jul 2019 00:26:42: 9000000 INFO @ Sat, 06 Jul 2019 00:26:44: 8000000 INFO @ Sat, 06 Jul 2019 00:26:45: 7000000 INFO @ Sat, 06 Jul 2019 00:26:50: 10000000 INFO @ Sat, 06 Jul 2019 00:26:54: 9000000 INFO @ Sat, 06 Jul 2019 00:26:55: 8000000 INFO @ Sat, 06 Jul 2019 00:26:58: 11000000 INFO @ Sat, 06 Jul 2019 00:27:03: 10000000 INFO @ Sat, 06 Jul 2019 00:27:05: 9000000 INFO @ Sat, 06 Jul 2019 00:27:06: 12000000 INFO @ Sat, 06 Jul 2019 00:27:11: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:27:11: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:27:11: #1 total tags in treatment: 6045532 INFO @ Sat, 06 Jul 2019 00:27:11: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:27:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:27:11: #1 tags after filtering in treatment: 4871795 INFO @ Sat, 06 Jul 2019 00:27:11: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:27:11: #1 finished! INFO @ Sat, 06 Jul 2019 00:27:11: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:27:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:27:12: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:27:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Sat, 06 Jul 2019 00:27:12: 11000000 WARNING @ Sat, 06 Jul 2019 00:27:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:27:16: 10000000 INFO @ Sat, 06 Jul 2019 00:27:21: 12000000 INFO @ Sat, 06 Jul 2019 00:27:26: 11000000 INFO @ Sat, 06 Jul 2019 00:27:26: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:27:26: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:27:26: #1 total tags in treatment: 6045532 INFO @ Sat, 06 Jul 2019 00:27:26: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:27:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:27:26: #1 tags after filtering in treatment: 4871795 INFO @ Sat, 06 Jul 2019 00:27:26: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:27:26: #1 finished! INFO @ Sat, 06 Jul 2019 00:27:26: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:27:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:27:27: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:27:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:27:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:27:36: 12000000 INFO @ Sat, 06 Jul 2019 00:27:42: #1 tag size is determined as 75 bps INFO @ Sat, 06 Jul 2019 00:27:42: #1 tag size = 75 INFO @ Sat, 06 Jul 2019 00:27:42: #1 total tags in treatment: 6045532 INFO @ Sat, 06 Jul 2019 00:27:42: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:27:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:27:42: #1 tags after filtering in treatment: 4871795 INFO @ Sat, 06 Jul 2019 00:27:42: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 06 Jul 2019 00:27:42: #1 finished! INFO @ Sat, 06 Jul 2019 00:27:42: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:27:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:27:42: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:27:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:27:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386896/SRX386896.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。