Job ID = 2010643 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 1,534,822 reads read : 3,069,644 reads written : 3,069,644 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 1534822 reads; of these: 1534822 (100.00%) were paired; of these: 1059759 (69.05%) aligned concordantly 0 times 387024 (25.22%) aligned concordantly exactly 1 time 88039 (5.74%) aligned concordantly >1 times ---- 1059759 pairs aligned concordantly 0 times; of these: 5698 (0.54%) aligned discordantly 1 time ---- 1054061 pairs aligned 0 times concordantly or discordantly; of these: 2108122 mates make up the pairs; of these: 1992802 (94.53%) aligned 0 times 92279 (4.38%) aligned exactly 1 time 23041 (1.09%) aligned >1 times 35.08% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 230853 / 478496 = 0.4825 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 23:48:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:26: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:26: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:48:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:48:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:48:32: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:48:32: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:48:32: #1 total tags in treatment: 246365 INFO @ Fri, 05 Jul 2019 23:48:32: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:48:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:48:32: #1 tags after filtering in treatment: 199927 INFO @ Fri, 05 Jul 2019 23:48:32: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:48:32: #1 finished! INFO @ Fri, 05 Jul 2019 23:48:32: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:48:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:48:32: #2 number of paired peaks: 159 WARNING @ Fri, 05 Jul 2019 23:48:32: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Fri, 05 Jul 2019 23:48:32: start model_add_line... INFO @ Fri, 05 Jul 2019 23:48:32: start X-correlation... INFO @ Fri, 05 Jul 2019 23:48:32: end of X-cor INFO @ Fri, 05 Jul 2019 23:48:32: #2 finished! INFO @ Fri, 05 Jul 2019 23:48:32: #2 predicted fragment length is 234 bps INFO @ Fri, 05 Jul 2019 23:48:32: #2 alternative fragment length(s) may be 199,234 bps INFO @ Fri, 05 Jul 2019 23:48:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05_model.r INFO @ Fri, 05 Jul 2019 23:48:32: #3 Call peaks... INFO @ Fri, 05 Jul 2019 23:48:32: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 23:48:33: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 23:48:33: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05_peaks.xls INFO @ Fri, 05 Jul 2019 23:48:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 23:48:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.05_summits.bed INFO @ Fri, 05 Jul 2019 23:48:33: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (128 records, 4 fields): 4 millis INFO @ Fri, 05 Jul 2019 23:48:33: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:48:33: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:48:33: #1 total tags in treatment: 246365 INFO @ Fri, 05 Jul 2019 23:48:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:48:33: #1 tags after filtering in treatment: 199927 INFO @ Fri, 05 Jul 2019 23:48:33: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:48:33: #1 finished! INFO @ Fri, 05 Jul 2019 23:48:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:48:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:48:33: #2 number of paired peaks: 159 WARNING @ Fri, 05 Jul 2019 23:48:33: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Fri, 05 Jul 2019 23:48:33: start model_add_line... INFO @ Fri, 05 Jul 2019 23:48:33: start X-correlation... INFO @ Fri, 05 Jul 2019 23:48:33: end of X-cor INFO @ Fri, 05 Jul 2019 23:48:33: #2 finished! INFO @ Fri, 05 Jul 2019 23:48:33: #2 predicted fragment length is 234 bps INFO @ Fri, 05 Jul 2019 23:48:33: #2 alternative fragment length(s) may be 199,234 bps INFO @ Fri, 05 Jul 2019 23:48:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10_model.r INFO @ Fri, 05 Jul 2019 23:48:33: #3 Call peaks... INFO @ Fri, 05 Jul 2019 23:48:33: #3 Pre-compute pvalue-qvalue table... CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 23:48:34: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:48:34: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:48:34: #1 total tags in treatment: 246365 INFO @ Fri, 05 Jul 2019 23:48:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:48:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:48:34: #1 tags after filtering in treatment: 199927 INFO @ Fri, 05 Jul 2019 23:48:34: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:48:34: #1 finished! INFO @ Fri, 05 Jul 2019 23:48:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:48:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:48:34: #2 number of paired peaks: 159 WARNING @ Fri, 05 Jul 2019 23:48:34: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Fri, 05 Jul 2019 23:48:34: start model_add_line... INFO @ Fri, 05 Jul 2019 23:48:34: start X-correlation... INFO @ Fri, 05 Jul 2019 23:48:34: end of X-cor INFO @ Fri, 05 Jul 2019 23:48:34: #2 finished! INFO @ Fri, 05 Jul 2019 23:48:34: #2 predicted fragment length is 234 bps INFO @ Fri, 05 Jul 2019 23:48:34: #2 alternative fragment length(s) may be 199,234 bps INFO @ Fri, 05 Jul 2019 23:48:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20_model.r INFO @ Fri, 05 Jul 2019 23:48:34: #3 Call peaks... INFO @ Fri, 05 Jul 2019 23:48:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 23:48:34: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 23:48:34: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10_peaks.xls INFO @ Fri, 05 Jul 2019 23:48:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 23:48:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.10_summits.bed INFO @ Fri, 05 Jul 2019 23:48:34: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (61 records, 4 fields): 3 millis INFO @ Fri, 05 Jul 2019 23:48:35: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 23:48:35: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20_peaks.xls INFO @ Fri, 05 Jul 2019 23:48:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 23:48:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX386372/SRX386372.20_summits.bed INFO @ Fri, 05 Jul 2019 23:48:35: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (15 records, 4 fields): 2 millis CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。