Job ID = 2010638 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 2,542,631 reads read : 5,085,262 reads written : 5,085,262 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:23 2542631 reads; of these: 2542631 (100.00%) were paired; of these: 1419415 (55.82%) aligned concordantly 0 times 855344 (33.64%) aligned concordantly exactly 1 time 267872 (10.54%) aligned concordantly >1 times ---- 1419415 pairs aligned concordantly 0 times; of these: 7956 (0.56%) aligned discordantly 1 time ---- 1411459 pairs aligned 0 times concordantly or discordantly; of these: 2822918 mates make up the pairs; of these: 2641935 (93.59%) aligned 0 times 130591 (4.63%) aligned exactly 1 time 50392 (1.79%) aligned >1 times 48.05% overall alignment rate Time searching: 00:01:24 Overall time: 00:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 443519 / 1127112 = 0.3935 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 23:48:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:55: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:55: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:48:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:56: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:56: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:48:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:48:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:48:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:49:04: 1000000 INFO @ Fri, 05 Jul 2019 23:49:06: 1000000 INFO @ Fri, 05 Jul 2019 23:49:06: 1000000 INFO @ Fri, 05 Jul 2019 23:49:08: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:49:08: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:49:08: #1 total tags in treatment: 681792 INFO @ Fri, 05 Jul 2019 23:49:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:49:08: #1 tags after filtering in treatment: 550809 INFO @ Fri, 05 Jul 2019 23:49:08: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:49:08: #1 finished! INFO @ Fri, 05 Jul 2019 23:49:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:49:08: #2 number of paired peaks: 65 WARNING @ Fri, 05 Jul 2019 23:49:08: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:49:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 23:49:11: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:49:11: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:49:11: #1 total tags in treatment: 681792 INFO @ Fri, 05 Jul 2019 23:49:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:49:11: #1 tags after filtering in treatment: 550809 INFO @ Fri, 05 Jul 2019 23:49:11: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:49:11: #1 finished! INFO @ Fri, 05 Jul 2019 23:49:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:49:11: #2 number of paired peaks: 65 WARNING @ Fri, 05 Jul 2019 23:49:11: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:49:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 23:49:11: #1 tag size is determined as 70 bps INFO @ Fri, 05 Jul 2019 23:49:11: #1 tag size = 70 INFO @ Fri, 05 Jul 2019 23:49:11: #1 total tags in treatment: 681792 INFO @ Fri, 05 Jul 2019 23:49:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:49:11: #1 tags after filtering in treatment: 550809 INFO @ Fri, 05 Jul 2019 23:49:11: #1 Redundant rate of treatment: 0.19 INFO @ Fri, 05 Jul 2019 23:49:11: #1 finished! INFO @ Fri, 05 Jul 2019 23:49:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:49:12: #2 number of paired peaks: 65 WARNING @ Fri, 05 Jul 2019 23:49:12: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:49:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX386365/SRX386365.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。