Job ID = 11296935 sra ファイルのダウンロード中... Completed: 140854K bytes transferred in 4 seconds (231439K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 7122745 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3856350/SRR6908167.sra Written 7122745 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3856350/SRR6908167.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 7122745 reads; of these: 7122745 (100.00%) were unpaired; of these: 181816 (2.55%) aligned 0 times 5794602 (81.35%) aligned exactly 1 time 1146327 (16.09%) aligned >1 times 97.45% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1522771 / 6940929 = 0.2194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 18:00:46: # Command line: callpeak -t SRX3856350.bam -f BAM -g 12100000 -n SRX3856350.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3856350.20 # format = BAM # ChIP-seq file = ['SRX3856350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:00:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:00:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:00:46: # Command line: callpeak -t SRX3856350.bam -f BAM -g 12100000 -n SRX3856350.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3856350.10 # format = BAM # ChIP-seq file = ['SRX3856350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:00:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:00:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:00:46: # Command line: callpeak -t SRX3856350.bam -f BAM -g 12100000 -n SRX3856350.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3856350.05 # format = BAM # ChIP-seq file = ['SRX3856350.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 18:00:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 18:00:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 18:00:52: 1000000 INFO @ Mon, 05 Nov 2018 18:00:53: 1000000 INFO @ Mon, 05 Nov 2018 18:00:53: 1000000 INFO @ Mon, 05 Nov 2018 18:00:59: 2000000 INFO @ Mon, 05 Nov 2018 18:00:59: 2000000 INFO @ Mon, 05 Nov 2018 18:00:59: 2000000 INFO @ Mon, 05 Nov 2018 18:01:05: 3000000 INFO @ Mon, 05 Nov 2018 18:01:05: 3000000 INFO @ Mon, 05 Nov 2018 18:01:05: 3000000 INFO @ Mon, 05 Nov 2018 18:01:11: 4000000 INFO @ Mon, 05 Nov 2018 18:01:12: 4000000 INFO @ Mon, 05 Nov 2018 18:01:12: 4000000 INFO @ Mon, 05 Nov 2018 18:01:18: 5000000 INFO @ Mon, 05 Nov 2018 18:01:18: 5000000 INFO @ Mon, 05 Nov 2018 18:01:18: 5000000 INFO @ Mon, 05 Nov 2018 18:01:20: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 18:01:20: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 18:01:20: #1 total tags in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:20: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:01:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:01:20: #1 tags after filtering in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:01:20: #1 finished! INFO @ Mon, 05 Nov 2018 18:01:20: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:01:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:01:21: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 18:01:21: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 18:01:21: #1 total tags in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:21: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:01:21: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 18:01:21: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 18:01:21: #1 total tags in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:21: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 18:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 18:01:21: #1 tags after filtering in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:01:21: #1 finished! INFO @ Mon, 05 Nov 2018 18:01:21: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:01:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:01:21: #1 tags after filtering in treatment: 5418158 INFO @ Mon, 05 Nov 2018 18:01:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 18:01:21: #1 finished! INFO @ Mon, 05 Nov 2018 18:01:21: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 18:01:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 18:01:21: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 18:01:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:01:21: Process for pairing-model is terminated! cat: SRX3856350.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3856350.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 18:01:21: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 18:01:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:01:21: Process for pairing-model is terminated! cat: SRX3856350.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3856350.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 18:01:21: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 18:01:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 18:01:21: Process for pairing-model is terminated! cat: SRX3856350.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3856350.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3856350.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。