Job ID = 2010609 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T14:33:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:33:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 11,778,177 reads read : 23,556,354 reads written : 11,778,177 reads 0-length : 11,778,177 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:39 11778177 reads; of these: 11778177 (100.00%) were unpaired; of these: 4304703 (36.55%) aligned 0 times 6701248 (56.90%) aligned exactly 1 time 772226 (6.56%) aligned >1 times 63.45% overall alignment rate Time searching: 00:01:39 Overall time: 00:01:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1933423 / 7473474 = 0.2587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 23:41:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:41:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:41:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:41:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:41:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:41:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:41:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 23:41:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 23:41:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 23:41:24: 1000000 INFO @ Fri, 05 Jul 2019 23:41:24: 1000000 INFO @ Fri, 05 Jul 2019 23:41:25: 1000000 INFO @ Fri, 05 Jul 2019 23:41:31: 2000000 INFO @ Fri, 05 Jul 2019 23:41:32: 2000000 INFO @ Fri, 05 Jul 2019 23:41:32: 2000000 INFO @ Fri, 05 Jul 2019 23:41:38: 3000000 INFO @ Fri, 05 Jul 2019 23:41:38: 3000000 INFO @ Fri, 05 Jul 2019 23:41:39: 3000000 INFO @ Fri, 05 Jul 2019 23:41:44: 4000000 INFO @ Fri, 05 Jul 2019 23:41:45: 4000000 INFO @ Fri, 05 Jul 2019 23:41:45: 4000000 INFO @ Fri, 05 Jul 2019 23:41:51: 5000000 INFO @ Fri, 05 Jul 2019 23:41:52: 5000000 INFO @ Fri, 05 Jul 2019 23:41:52: 5000000 INFO @ Fri, 05 Jul 2019 23:41:54: #1 tag size is determined as 33 bps INFO @ Fri, 05 Jul 2019 23:41:54: #1 tag size = 33 INFO @ Fri, 05 Jul 2019 23:41:54: #1 total tags in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:41:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:41:54: #1 tags after filtering in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 23:41:54: #1 finished! INFO @ Fri, 05 Jul 2019 23:41:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:41:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:41:55: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 23:41:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:41:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 23:41:55: #1 tag size is determined as 33 bps INFO @ Fri, 05 Jul 2019 23:41:55: #1 tag size = 33 INFO @ Fri, 05 Jul 2019 23:41:55: #1 total tags in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:55: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:41:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 23:41:55: #1 tags after filtering in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 23:41:55: #1 finished! INFO @ Fri, 05 Jul 2019 23:41:55: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:41:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:41:56: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 23:41:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:41:56: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 23:41:56: #1 tag size is determined as 33 bps INFO @ Fri, 05 Jul 2019 23:41:56: #1 tag size = 33 INFO @ Fri, 05 Jul 2019 23:41:56: #1 total tags in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 23:41:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 23:41:56: #1 tags after filtering in treatment: 5540051 INFO @ Fri, 05 Jul 2019 23:41:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 23:41:56: #1 finished! INFO @ Fri, 05 Jul 2019 23:41:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 23:41:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 23:41:56: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 23:41:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 23:41:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3823260/SRX3823260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。