Job ID = 2010545 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T13:56:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:02:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:05:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:14:26 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:16:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:21:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:21:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:22:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:24:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:25:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:26:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:27:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:30:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:32:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 33,701,332 reads read : 67,402,664 reads written : 67,402,664 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:30 33701332 reads; of these: 33701332 (100.00%) were paired; of these: 8563976 (25.41%) aligned concordantly 0 times 18984474 (56.33%) aligned concordantly exactly 1 time 6152882 (18.26%) aligned concordantly >1 times ---- 8563976 pairs aligned concordantly 0 times; of these: 332389 (3.88%) aligned discordantly 1 time ---- 8231587 pairs aligned 0 times concordantly or discordantly; of these: 16463174 mates make up the pairs; of these: 15780273 (95.85%) aligned 0 times 321471 (1.95%) aligned exactly 1 time 361430 (2.20%) aligned >1 times 76.59% overall alignment rate Time searching: 00:27:30 Overall time: 00:27:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 11078892 / 25437882 = 0.4355 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:19:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:19:55: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:19:55: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:19:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:19:56: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:19:56: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:19:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:19:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:19:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:20:02: 1000000 INFO @ Sat, 06 Jul 2019 00:20:03: 1000000 INFO @ Sat, 06 Jul 2019 00:20:05: 1000000 INFO @ Sat, 06 Jul 2019 00:20:08: 2000000 INFO @ Sat, 06 Jul 2019 00:20:11: 2000000 INFO @ Sat, 06 Jul 2019 00:20:12: 2000000 INFO @ Sat, 06 Jul 2019 00:20:15: 3000000 INFO @ Sat, 06 Jul 2019 00:20:19: 3000000 INFO @ Sat, 06 Jul 2019 00:20:20: 3000000 INFO @ Sat, 06 Jul 2019 00:20:21: 4000000 INFO @ Sat, 06 Jul 2019 00:20:27: 4000000 INFO @ Sat, 06 Jul 2019 00:20:28: 4000000 INFO @ Sat, 06 Jul 2019 00:20:28: 5000000 INFO @ Sat, 06 Jul 2019 00:20:34: 5000000 INFO @ Sat, 06 Jul 2019 00:20:34: 6000000 INFO @ Sat, 06 Jul 2019 00:20:35: 5000000 INFO @ Sat, 06 Jul 2019 00:20:41: 7000000 INFO @ Sat, 06 Jul 2019 00:20:42: 6000000 INFO @ Sat, 06 Jul 2019 00:20:43: 6000000 INFO @ Sat, 06 Jul 2019 00:20:47: 8000000 INFO @ Sat, 06 Jul 2019 00:20:50: 7000000 INFO @ Sat, 06 Jul 2019 00:20:51: 7000000 INFO @ Sat, 06 Jul 2019 00:20:53: 9000000 INFO @ Sat, 06 Jul 2019 00:20:57: 8000000 INFO @ Sat, 06 Jul 2019 00:20:58: 8000000 INFO @ Sat, 06 Jul 2019 00:20:59: 10000000 INFO @ Sat, 06 Jul 2019 00:21:05: 9000000 INFO @ Sat, 06 Jul 2019 00:21:05: 11000000 INFO @ Sat, 06 Jul 2019 00:21:05: 9000000 INFO @ Sat, 06 Jul 2019 00:21:12: 12000000 INFO @ Sat, 06 Jul 2019 00:21:12: 10000000 INFO @ Sat, 06 Jul 2019 00:21:13: 10000000 INFO @ Sat, 06 Jul 2019 00:21:18: 13000000 INFO @ Sat, 06 Jul 2019 00:21:20: 11000000 INFO @ Sat, 06 Jul 2019 00:21:21: 11000000 INFO @ Sat, 06 Jul 2019 00:21:24: 14000000 INFO @ Sat, 06 Jul 2019 00:21:27: 12000000 INFO @ Sat, 06 Jul 2019 00:21:28: 12000000 INFO @ Sat, 06 Jul 2019 00:21:31: 15000000 INFO @ Sat, 06 Jul 2019 00:21:34: 13000000 INFO @ Sat, 06 Jul 2019 00:21:35: 13000000 INFO @ Sat, 06 Jul 2019 00:21:37: 16000000 INFO @ Sat, 06 Jul 2019 00:21:42: 14000000 INFO @ Sat, 06 Jul 2019 00:21:43: 14000000 INFO @ Sat, 06 Jul 2019 00:21:43: 17000000 INFO @ Sat, 06 Jul 2019 00:21:49: 15000000 INFO @ Sat, 06 Jul 2019 00:21:50: 18000000 INFO @ Sat, 06 Jul 2019 00:21:50: 15000000 INFO @ Sat, 06 Jul 2019 00:21:56: 19000000 INFO @ Sat, 06 Jul 2019 00:21:56: 16000000 INFO @ Sat, 06 Jul 2019 00:21:57: 16000000 INFO @ Sat, 06 Jul 2019 00:22:02: 20000000 INFO @ Sat, 06 Jul 2019 00:22:03: 17000000 INFO @ Sat, 06 Jul 2019 00:22:04: 17000000 INFO @ Sat, 06 Jul 2019 00:22:08: 21000000 INFO @ Sat, 06 Jul 2019 00:22:10: 18000000 INFO @ Sat, 06 Jul 2019 00:22:11: 18000000 INFO @ Sat, 06 Jul 2019 00:22:14: 22000000 INFO @ Sat, 06 Jul 2019 00:22:17: 19000000 INFO @ Sat, 06 Jul 2019 00:22:18: 19000000 INFO @ Sat, 06 Jul 2019 00:22:20: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:22:25: 20000000 INFO @ Sat, 06 Jul 2019 00:22:25: 20000000 INFO @ Sat, 06 Jul 2019 00:22:26: 24000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 00:22:32: 21000000 INFO @ Sat, 06 Jul 2019 00:22:32: 25000000 INFO @ Sat, 06 Jul 2019 00:22:32: 21000000 INFO @ Sat, 06 Jul 2019 00:22:38: 26000000 INFO @ Sat, 06 Jul 2019 00:22:39: 22000000 INFO @ Sat, 06 Jul 2019 00:22:40: 22000000 INFO @ Sat, 06 Jul 2019 00:22:44: 27000000 INFO @ Sat, 06 Jul 2019 00:22:46: 23000000 INFO @ Sat, 06 Jul 2019 00:22:47: 23000000 INFO @ Sat, 06 Jul 2019 00:22:51: 28000000 INFO @ Sat, 06 Jul 2019 00:22:53: 24000000 INFO @ Sat, 06 Jul 2019 00:22:54: 24000000 INFO @ Sat, 06 Jul 2019 00:22:57: 29000000 INFO @ Sat, 06 Jul 2019 00:23:00: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:23:00: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:23:00: #1 total tags in treatment: 14139562 INFO @ Sat, 06 Jul 2019 00:23:00: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:23:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:23:00: #1 tags after filtering in treatment: 4141027 INFO @ Sat, 06 Jul 2019 00:23:00: #1 Redundant rate of treatment: 0.71 INFO @ Sat, 06 Jul 2019 00:23:00: #1 finished! INFO @ Sat, 06 Jul 2019 00:23:00: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:23:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:23:01: 25000000 INFO @ Sat, 06 Jul 2019 00:23:01: #2 number of paired peaks: 1080 INFO @ Sat, 06 Jul 2019 00:23:01: start model_add_line... INFO @ Sat, 06 Jul 2019 00:23:01: start X-correlation... INFO @ Sat, 06 Jul 2019 00:23:01: end of X-cor INFO @ Sat, 06 Jul 2019 00:23:01: #2 finished! INFO @ Sat, 06 Jul 2019 00:23:01: #2 predicted fragment length is 22 bps INFO @ Sat, 06 Jul 2019 00:23:01: #2 alternative fragment length(s) may be 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 bps INFO @ Sat, 06 Jul 2019 00:23:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05_model.r WARNING @ Sat, 06 Jul 2019 00:23:01: #2 Since the d (22) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 06 Jul 2019 00:23:01: #2 You may need to consider one of the other alternative d(s): 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 WARNING @ Sat, 06 Jul 2019 00:23:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 06 Jul 2019 00:23:01: #3 Call peaks... INFO @ Sat, 06 Jul 2019 00:23:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 06 Jul 2019 00:23:02: 25000000 INFO @ Sat, 06 Jul 2019 00:23:08: 26000000 INFO @ Sat, 06 Jul 2019 00:23:09: #3 Call peaks for each chromosome... INFO @ Sat, 06 Jul 2019 00:23:09: 26000000 INFO @ Sat, 06 Jul 2019 00:23:12: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05_peaks.xls INFO @ Sat, 06 Jul 2019 00:23:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05_peaks.narrowPeak INFO @ Sat, 06 Jul 2019 00:23:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.05_summits.bed INFO @ Sat, 06 Jul 2019 00:23:12: Done! pass1 - making usageList (13 chroms): 0 millis pass2 - checking and writing primary data (61 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:23:15: 27000000 INFO @ Sat, 06 Jul 2019 00:23:16: 27000000 INFO @ Sat, 06 Jul 2019 00:23:22: 28000000 INFO @ Sat, 06 Jul 2019 00:23:23: 28000000 INFO @ Sat, 06 Jul 2019 00:23:29: 29000000 INFO @ Sat, 06 Jul 2019 00:23:30: 29000000 INFO @ Sat, 06 Jul 2019 00:23:33: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:23:33: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:23:33: #1 total tags in treatment: 14139562 INFO @ Sat, 06 Jul 2019 00:23:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:23:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:23:33: #1 tags after filtering in treatment: 4141027 INFO @ Sat, 06 Jul 2019 00:23:33: #1 Redundant rate of treatment: 0.71 INFO @ Sat, 06 Jul 2019 00:23:33: #1 finished! INFO @ Sat, 06 Jul 2019 00:23:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:23:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:23:34: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:23:34: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:23:34: #1 total tags in treatment: 14139562 INFO @ Sat, 06 Jul 2019 00:23:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:23:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:23:34: #2 number of paired peaks: 1080 INFO @ Sat, 06 Jul 2019 00:23:34: start model_add_line... INFO @ Sat, 06 Jul 2019 00:23:34: start X-correlation... INFO @ Sat, 06 Jul 2019 00:23:34: end of X-cor INFO @ Sat, 06 Jul 2019 00:23:34: #2 finished! INFO @ Sat, 06 Jul 2019 00:23:34: #2 predicted fragment length is 22 bps INFO @ Sat, 06 Jul 2019 00:23:34: #2 alternative fragment length(s) may be 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 bps INFO @ Sat, 06 Jul 2019 00:23:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10_model.r WARNING @ Sat, 06 Jul 2019 00:23:34: #2 Since the d (22) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 06 Jul 2019 00:23:34: #2 You may need to consider one of the other alternative d(s): 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 WARNING @ Sat, 06 Jul 2019 00:23:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 06 Jul 2019 00:23:34: #3 Call peaks... INFO @ Sat, 06 Jul 2019 00:23:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 06 Jul 2019 00:23:34: #1 tags after filtering in treatment: 4141027 INFO @ Sat, 06 Jul 2019 00:23:34: #1 Redundant rate of treatment: 0.71 INFO @ Sat, 06 Jul 2019 00:23:34: #1 finished! INFO @ Sat, 06 Jul 2019 00:23:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:23:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:23:34: #2 number of paired peaks: 1080 INFO @ Sat, 06 Jul 2019 00:23:34: start model_add_line... INFO @ Sat, 06 Jul 2019 00:23:34: start X-correlation... INFO @ Sat, 06 Jul 2019 00:23:35: end of X-cor INFO @ Sat, 06 Jul 2019 00:23:35: #2 finished! INFO @ Sat, 06 Jul 2019 00:23:35: #2 predicted fragment length is 22 bps INFO @ Sat, 06 Jul 2019 00:23:35: #2 alternative fragment length(s) may be 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 bps INFO @ Sat, 06 Jul 2019 00:23:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20_model.r WARNING @ Sat, 06 Jul 2019 00:23:35: #2 Since the d (22) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 06 Jul 2019 00:23:35: #2 You may need to consider one of the other alternative d(s): 22,45,73,94,114,143,165,206,227,255,299,313,398,443,469,529,557,578 WARNING @ Sat, 06 Jul 2019 00:23:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 06 Jul 2019 00:23:35: #3 Call peaks... INFO @ Sat, 06 Jul 2019 00:23:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 06 Jul 2019 00:23:41: #3 Call peaks for each chromosome... INFO @ Sat, 06 Jul 2019 00:23:42: #3 Call peaks for each chromosome... INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10_peaks.xls INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10_peaks.narrowPeak INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.10_summits.bed INFO @ Sat, 06 Jul 2019 00:23:45: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20_peaks.xls INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20_peaks.narrowPeak INFO @ Sat, 06 Jul 2019 00:23:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX381247/SRX381247.20_summits.bed INFO @ Sat, 06 Jul 2019 00:23:45: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling