Job ID = 2010530 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 42,192,494 reads read : 84,384,988 reads written : 84,384,988 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:44 42192494 reads; of these: 42192494 (100.00%) were paired; of these: 4991160 (11.83%) aligned concordantly 0 times 25760691 (61.06%) aligned concordantly exactly 1 time 11440643 (27.12%) aligned concordantly >1 times ---- 4991160 pairs aligned concordantly 0 times; of these: 198860 (3.98%) aligned discordantly 1 time ---- 4792300 pairs aligned 0 times concordantly or discordantly; of these: 9584600 mates make up the pairs; of these: 8835801 (92.19%) aligned 0 times 368086 (3.84%) aligned exactly 1 time 380713 (3.97%) aligned >1 times 89.53% overall alignment rate Time searching: 00:39:44 Overall time: 00:39:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12023001 / 37367638 = 0.3217 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:40:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:40:00: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:40:00: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:40:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:40:01: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:40:01: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:40:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:40:02: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:40:02: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:40:06: 1000000 INFO @ Sat, 06 Jul 2019 00:40:09: 1000000 INFO @ Sat, 06 Jul 2019 00:40:10: 1000000 INFO @ Sat, 06 Jul 2019 00:40:13: 2000000 INFO @ Sat, 06 Jul 2019 00:40:17: 2000000 INFO @ Sat, 06 Jul 2019 00:40:18: 2000000 INFO @ Sat, 06 Jul 2019 00:40:19: 3000000 INFO @ Sat, 06 Jul 2019 00:40:25: 3000000 INFO @ Sat, 06 Jul 2019 00:40:25: 4000000 INFO @ Sat, 06 Jul 2019 00:40:27: 3000000 INFO @ Sat, 06 Jul 2019 00:40:31: 5000000 INFO @ Sat, 06 Jul 2019 00:40:32: 4000000 INFO @ Sat, 06 Jul 2019 00:40:35: 4000000 INFO @ Sat, 06 Jul 2019 00:40:37: 6000000 INFO @ Sat, 06 Jul 2019 00:40:40: 5000000 INFO @ Sat, 06 Jul 2019 00:40:43: 7000000 INFO @ Sat, 06 Jul 2019 00:40:44: 5000000 INFO @ Sat, 06 Jul 2019 00:40:48: 6000000 INFO @ Sat, 06 Jul 2019 00:40:50: 8000000 INFO @ Sat, 06 Jul 2019 00:40:52: 6000000 INFO @ Sat, 06 Jul 2019 00:40:55: 7000000 INFO @ Sat, 06 Jul 2019 00:40:56: 9000000 INFO @ Sat, 06 Jul 2019 00:41:01: 7000000 INFO @ Sat, 06 Jul 2019 00:41:02: 10000000 INFO @ Sat, 06 Jul 2019 00:41:03: 8000000 INFO @ Sat, 06 Jul 2019 00:41:08: 11000000 INFO @ Sat, 06 Jul 2019 00:41:10: 8000000 INFO @ Sat, 06 Jul 2019 00:41:12: 9000000 INFO @ Sat, 06 Jul 2019 00:41:14: 12000000 INFO @ Sat, 06 Jul 2019 00:41:18: 9000000 INFO @ Sat, 06 Jul 2019 00:41:20: 10000000 INFO @ Sat, 06 Jul 2019 00:41:20: 13000000 INFO @ Sat, 06 Jul 2019 00:41:26: 14000000 INFO @ Sat, 06 Jul 2019 00:41:27: 10000000 INFO @ Sat, 06 Jul 2019 00:41:27: 11000000 INFO @ Sat, 06 Jul 2019 00:41:32: 15000000 INFO @ Sat, 06 Jul 2019 00:41:35: 11000000 INFO @ Sat, 06 Jul 2019 00:41:35: 12000000 INFO @ Sat, 06 Jul 2019 00:41:38: 16000000 INFO @ Sat, 06 Jul 2019 00:41:43: 12000000 INFO @ Sat, 06 Jul 2019 00:41:43: 13000000 INFO @ Sat, 06 Jul 2019 00:41:45: 17000000 INFO @ Sat, 06 Jul 2019 00:41:51: 14000000 INFO @ Sat, 06 Jul 2019 00:41:51: 18000000 INFO @ Sat, 06 Jul 2019 00:41:52: 13000000 INFO @ Sat, 06 Jul 2019 00:41:57: 19000000 INFO @ Sat, 06 Jul 2019 00:41:59: 15000000 INFO @ Sat, 06 Jul 2019 00:42:00: 14000000 INFO @ Sat, 06 Jul 2019 00:42:03: 20000000 INFO @ Sat, 06 Jul 2019 00:42:06: 15000000 INFO @ Sat, 06 Jul 2019 00:42:07: 16000000 INFO @ Sat, 06 Jul 2019 00:42:09: 21000000 INFO @ Sat, 06 Jul 2019 00:42:12: 16000000 INFO @ Sat, 06 Jul 2019 00:42:13: 17000000 INFO @ Sat, 06 Jul 2019 00:42:15: 22000000 INFO @ Sat, 06 Jul 2019 00:42:19: 17000000 INFO @ Sat, 06 Jul 2019 00:42:21: 23000000 INFO @ Sat, 06 Jul 2019 00:42:21: 18000000 INFO @ Sat, 06 Jul 2019 00:42:26: 18000000 INFO @ Sat, 06 Jul 2019 00:42:27: 24000000 INFO @ Sat, 06 Jul 2019 00:42:29: 19000000 INFO @ Sat, 06 Jul 2019 00:42:32: 19000000 INFO @ Sat, 06 Jul 2019 00:42:33: 25000000 INFO @ Sat, 06 Jul 2019 00:42:36: 20000000 INFO @ Sat, 06 Jul 2019 00:42:38: 20000000 INFO @ Sat, 06 Jul 2019 00:42:39: 26000000 INFO @ Sat, 06 Jul 2019 00:42:44: 21000000 INFO @ Sat, 06 Jul 2019 00:42:44: 21000000 INFO @ Sat, 06 Jul 2019 00:42:45: 27000000 INFO @ Sat, 06 Jul 2019 00:42:50: 22000000 INFO @ Sat, 06 Jul 2019 00:42:51: 28000000 INFO @ Sat, 06 Jul 2019 00:42:52: 22000000 INFO @ Sat, 06 Jul 2019 00:42:56: 29000000 INFO @ Sat, 06 Jul 2019 00:42:57: 23000000 INFO @ Sat, 06 Jul 2019 00:43:00: 23000000 INFO @ Sat, 06 Jul 2019 00:43:02: 30000000 INFO @ Sat, 06 Jul 2019 00:43:03: 24000000 INFO @ Sat, 06 Jul 2019 00:43:07: 24000000 INFO @ Sat, 06 Jul 2019 00:43:08: 31000000 INFO @ Sat, 06 Jul 2019 00:43:09: 25000000 INFO @ Sat, 06 Jul 2019 00:43:14: 32000000 INFO @ Sat, 06 Jul 2019 00:43:15: 25000000 INFO @ Sat, 06 Jul 2019 00:43:15: 26000000 INFO @ Sat, 06 Jul 2019 00:43:20: 33000000 INFO @ Sat, 06 Jul 2019 00:43:21: 27000000 INFO @ Sat, 06 Jul 2019 00:43:22: 26000000 INFO @ Sat, 06 Jul 2019 00:43:25: 34000000 INFO @ Sat, 06 Jul 2019 00:43:26: 28000000 INFO @ Sat, 06 Jul 2019 00:43:29: 27000000 INFO @ Sat, 06 Jul 2019 00:43:31: 35000000 INFO @ Sat, 06 Jul 2019 00:43:32: 29000000 INFO @ Sat, 06 Jul 2019 00:43:37: 28000000 INFO @ Sat, 06 Jul 2019 00:43:37: 36000000 INFO @ Sat, 06 Jul 2019 00:43:38: 30000000 INFO @ Sat, 06 Jul 2019 00:43:43: 37000000 INFO @ Sat, 06 Jul 2019 00:43:44: 31000000 INFO @ Sat, 06 Jul 2019 00:43:44: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:43:49: 38000000 INFO @ Sat, 06 Jul 2019 00:43:50: 32000000 INFO @ Sat, 06 Jul 2019 00:43:51: 30000000 INFO @ Sat, 06 Jul 2019 00:43:55: 39000000 INFO @ Sat, 06 Jul 2019 00:43:55: 33000000 INFO @ Sat, 06 Jul 2019 00:43:58: 31000000 INFO @ Sat, 06 Jul 2019 00:44:01: 40000000 INFO @ Sat, 06 Jul 2019 00:44:01: 34000000 INFO @ Sat, 06 Jul 2019 00:44:06: 32000000 INFO @ Sat, 06 Jul 2019 00:44:07: 41000000 INFO @ Sat, 06 Jul 2019 00:44:07: 35000000 INFO @ Sat, 06 Jul 2019 00:44:13: 36000000 INFO @ Sat, 06 Jul 2019 00:44:13: 42000000 INFO @ Sat, 06 Jul 2019 00:44:14: 33000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 00:44:19: 37000000 INFO @ Sat, 06 Jul 2019 00:44:20: 43000000 INFO @ Sat, 06 Jul 2019 00:44:22: 34000000 INFO @ Sat, 06 Jul 2019 00:44:25: 38000000 INFO @ Sat, 06 Jul 2019 00:44:26: 44000000 INFO @ Sat, 06 Jul 2019 00:44:30: 35000000 INFO @ Sat, 06 Jul 2019 00:44:31: 39000000 INFO @ Sat, 06 Jul 2019 00:44:32: 45000000 INFO @ Sat, 06 Jul 2019 00:44:37: 40000000 INFO @ Sat, 06 Jul 2019 00:44:38: 36000000 INFO @ Sat, 06 Jul 2019 00:44:39: 46000000 INFO @ Sat, 06 Jul 2019 00:44:45: 41000000 INFO @ Sat, 06 Jul 2019 00:44:45: 37000000 INFO @ Sat, 06 Jul 2019 00:44:45: 47000000 INFO @ Sat, 06 Jul 2019 00:44:51: 48000000 INFO @ Sat, 06 Jul 2019 00:44:52: 42000000 INFO @ Sat, 06 Jul 2019 00:44:53: 38000000 INFO @ Sat, 06 Jul 2019 00:44:57: 49000000 INFO @ Sat, 06 Jul 2019 00:45:00: 43000000 INFO @ Sat, 06 Jul 2019 00:45:01: 39000000 INFO @ Sat, 06 Jul 2019 00:45:04: 50000000 INFO @ Sat, 06 Jul 2019 00:45:07: 44000000 INFO @ Sat, 06 Jul 2019 00:45:09: 40000000 INFO @ Sat, 06 Jul 2019 00:45:11: 51000000 INFO @ Sat, 06 Jul 2019 00:45:14: 45000000 INFO @ Sat, 06 Jul 2019 00:45:14: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:45:14: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:45:14: #1 total tags in treatment: 25194236 INFO @ Sat, 06 Jul 2019 00:45:14: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:45:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:45:15: #1 tags after filtering in treatment: 4455548 INFO @ Sat, 06 Jul 2019 00:45:15: #1 Redundant rate of treatment: 0.82 INFO @ Sat, 06 Jul 2019 00:45:15: #1 finished! INFO @ Sat, 06 Jul 2019 00:45:15: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:45:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:45:16: #2 number of paired peaks: 1378 INFO @ Sat, 06 Jul 2019 00:45:16: start model_add_line... INFO @ Sat, 06 Jul 2019 00:45:16: start X-correlation... INFO @ Sat, 06 Jul 2019 00:45:16: end of X-cor INFO @ Sat, 06 Jul 2019 00:45:16: #2 finished! INFO @ Sat, 06 Jul 2019 00:45:16: #2 predicted fragment length is 0 bps INFO @ Sat, 06 Jul 2019 00:45:16: #2 alternative fragment length(s) may be 0,12,26,69,111,128,151,180,203,285,433,444,484,505,523,536,539 bps INFO @ Sat, 06 Jul 2019 00:45:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX381233/SRX381233.05_model.r WARNING @ Sat, 06 Jul 2019 00:45:16: #2 Since the d (0) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 06 Jul 2019 00:45:16: #2 You may need to consider one of the other alternative d(s): 0,12,26,69,111,128,151,180,203,285,433,444,484,505,523,536,539 WARNING @ Sat, 06 Jul 2019 00:45:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 06 Jul 2019 00:45:16: #3 Call peaks... INFO @ Sat, 06 Jul 2019 00:45:16: #3 Pre-compute pvalue-qvalue table... ls: cannot access SRX381233.05.bed: No such file or directory mv: cannot stat ‘SRX381233.05.bed’: No such file or directory /var/spool/uge/at101/job_scripts/2010530: line 335: 6896 Terminated MACS $i /var/spool/uge/at101/job_scripts/2010530: line 335: 6912 Terminated MACS $i /var/spool/uge/at101/job_scripts/2010530: line 335: 6926 Terminated MACS $i mv: cannot stat ‘SRX381233.05.bb’: No such file or directory ls: cannot access SRX381233.10.bed: No such file or directory mv: cannot stat ‘SRX381233.10.bed’: No such file or directory mv: cannot stat ‘SRX381233.10.bb’: No such file or directory ls: cannot access SRX381233.20.bed: No such file or directory mv: cannot stat ‘SRX381233.20.bed’: No such file or directory mv: cannot stat ‘SRX381233.20.bb’: No such file or directory